Pretreatment-derived supernatants were re-centrifuged at 2465g and 200 μl of the supernatant was filtered using a syringe filter (0.2 µm, Whatman International Ltd, Maidstone, UK), and injected into vials. The concentrations of the fermentation inhibitors 2-furfuraldehyde (2-FA), 5-hydroxymethylfurfural (5-HMF), and the organic acids (formic and acetic acid) were analysed by an HPLC using a Flexar LC instrument (PerkinElmer, Seer Green, Bucks., UK) equipped with refractive index and photo diode array detectors (outputting chromatograms at 210, 280, and 325 nm wavelengths) in series. The analyses were carried out using an Aminex HPX-87H organic acid analysis column (Bio-Rad Laboratories Ltd, Hemel Hempstead, UK) operating at 65 °C with 0.004 mol/l H2SO4 (Sigma-Aldrich) as the mobile phase at a flow rate of 0.6 ml/min.
Flexar lc instrument
Manufactured by PerkinElmer
Sourced in United Kingdom
The Flexar LC instrument is a liquid chromatography system designed for analytical and preparative separation and purification of chemical compounds. It provides high-performance liquid chromatography (HPLC) functionality for a range of applications.
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Lab products found in correlation
3 protocols using flexar lc instrument
1
HPLC Analysis of Fermentation Inhibitors
2
Quantification of Fermentation Inhibitors
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Pretreatment-derived supernatants were recentrifuged (2465×g) and 200 μL of the supernatant syringe filtered into vials (0.2 µm, Whatman International Ltd, Maidstone, UK). The concentration of the fermentation inhibitors 2-furfuraldehyde (2-FA), 5-Hydroxymethylfurfural (5-HMF) and the organic acids (formic and acetic acid) were analysed by HPLC using a Flexar LC instrument (PerkinElmer, Seer Green, Bucks., UK) equipped with refractive index and photo diode array detectors (reading at 210 nm wavelength) in series. The analyses were carried out using an Aminex HPX-87H carbohydrate analysis column (Bio-Rad Laboratories Ltd, Hemel Hempstead, UK) operating at 65 °C with 0.005 mol/L H2SO4 (Sigma-Aldrich) as mobile phase at a flow rate of 0.6 mL/min.
3
HPLC Analysis of Fermentation Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2-furfuraldehyde (2-FA), 5-Hydroxymethylfurfural (5-HMF) and organic acids (formic and acetic acid) were assessed to help understand their impact on ethanol yields. Steam-exploded slurry (2 mL) and SSF products were centrifuged at 2465×g and 200 μL of the supernatant was filtered using a syringe filter (0.2 μm, Whatman International Ltd, Maidstone, UK), and injected into vials. The concentration of inhibitors was analysed by HPLC using a Flexar LC instrument (PerkinElmer, Seer Green, Bucks., UK) equipped with refractive index and photo diode array detectors (reading at 210 nm wavelength) in series. The analyses were carried out using an Aminex HPX-87H carbohydrate analysis column (Bio-Rad Laboratories Ltd, Hemel Hempstead, UK) operating at 65 °C with 0.005 mol L−1 H2SO4 (Sigma–Aldrich, 2009 ) as mobile phase at a flow rate of 0.6 mL min−1.
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