The largest database of trusted experimental protocols

Micromanipulator mmo 4

Manufactured by Narishige

The Narishige Micromanipulator MMO-4 is a precision instrument designed for accurate positioning and control of fine-tipped tools or probes. It features three-dimensional movement with micrometer control, allowing for precise manipulation of samples or specimens during various laboratory procedures.

Automatically generated - may contain errors

2 protocols using micromanipulator mmo 4

1

Generating Transgenic C. elegans Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate transgenic lines for localization, rescue experiments, and expression patterns, we generated the following transgenic animals via microinjections.
OIK1042 turEx21[arl-13p::wdr-31 (T05A8.5)::GFP::unc-54 3′UTR +rol-6}; T05A8.5(syb1568)II., elmd-1(syb630) II, rpi-2(K08D12.2)(ok1863) IV. (1 ng).
OIK1044 N2;turEx23[elmd-1p::GFP::elmd-1 (C56G7.3)::unc-54 3′UTR +rol-6} (5 ng).
OIK1045 N2;turEx24[arl-13p::GFP::elmd-1 (C56G7.3)::unc-54 3′UTR +rol-6} (5 ng).
OIK1046 N2;turEx25[elmd-1p(C56G7.3)::GFP::unc-54 3′UTR +rol-6} (50 ng).
OIK1047 N2;turEx26[wdr-31 (T05A8.5)p::GFP::unc-54 3′UTR +rol-6} (50 ng).
The rol-6 plasmid (50 ng/μl plasmid pRF4) was co-injected as the co-transformation marker. In brief, the plasmids were delivered by microinjections into the gonads of 1-d adult worms. Worms were initially transferred onto a 2.5% agarose pad before (Halocarbon oil, 9002-83-9; Sigma-Aldrich), followed by microinjection. The microinjection was done using a Zeiss Axio Vert.A1 inverted microscope with DIC optics coupled with a Narishige Micromanipulator MMO-4. We next manually inspected the plates to find successful transgenic animals.
+ Open protocol
+ Expand
2

Generating Transgenic C. elegans Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate transgenic lines for localization, rescue experiments, and expression patterns, we generated the following transgenic animals via microinjections. The rol-6 plasmid (50 ng/ μl plasmid pRF4) was co-injected as the co-transformation marker. In brief, the plasmids were delivered by microinjections into the gonads of 1-day adult worms.
Worms were initially transferred onto a 2.5% agarose pad prior (Halocarbon oil, Sigma: 9002-83-9), followed by microinjection. The microinjection was done using a Zeiss Axio Vert.A1 inverted microscope with DIC optics coupled with a Narishige Micromanipulator MMO-4. We next manually inspected the plates to find successful transgenics animals. Fluorescent transgenes for ciliary proteins: PHX1180, wdr-31(syb1180 [wdr-31(T05A8.5 )::GFP]); PHX1328, wdr-31(syb1328[wdr-31( R261-de) )::GFP]) (Disease mimicking variant); OIK1042 N2;turEx21 [arl-13p::wdr-31 (T05A8.5) ; unc122p::dsRed] . All PHX strains were generated using CRISPR/Cas9 by Sunybiotech. The list of extensive transgenic and mutant strains was provided in Table S2.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!