Micromanipulator mmo 4

Manufactured by Narishige

The Narishige Micromanipulator MMO-4 is a precision instrument designed for accurate positioning and control of fine-tipped tools or probes. It features three-dimensional movement with micrometer control, allowing for precise manipulation of samples or specimens during various laboratory procedures.

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Lab products found in correlation

Halocarbon oil, by Merck Group (2 mentions) Axio vert a1 inverted microscope, by Narishige (2 mentions)

2 protocols using micromanipulator mmo 4

Generating Transgenic C. elegans Strains

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To generate transgenic lines for localization, rescue experiments, and expression patterns, we generated the following transgenic animals via microinjections.
OIK1042 turEx21[arl-13p::wdr-31 (T05A8.5)::GFP::unc-54 3′UTR +rol-6}; T05A8.5(syb1568)II., elmd-1(syb630) II, rpi-2(K08D12.2)(ok1863) IV. (1 ng).
OIK1044 N2;turEx23[elmd-1p::GFP::elmd-1 (C56G7.3)::unc-54 3′UTR +rol-6} (5 ng).
OIK1045 N2;turEx24[arl-13p::GFP::elmd-1 (C56G7.3)::unc-54 3′UTR +rol-6} (5 ng).
OIK1046 N2;turEx25[elmd-1p(C56G7.3)::GFP::unc-54 3′UTR +rol-6} (50 ng).
OIK1047 N2;turEx26[wdr-31 (T05A8.5)p::GFP::unc-54 3′UTR +rol-6} (50 ng).
The rol-6 plasmid (50 ng/μl plasmid pRF4) was co-injected as the co-transformation marker. In brief, the plasmids were delivered by microinjections into the gonads of 1-d adult worms. Worms were initially transferred onto a 2.5% agarose pad before (Halocarbon oil, 9002-83-9; Sigma-Aldrich), followed by microinjection. The microinjection was done using a Zeiss Axio Vert.A1 inverted microscope with DIC optics coupled with a Narishige Micromanipulator MMO-4. We next manually inspected the plates to find successful transgenic animals.

Cevik S., Peng X., Beyer T., Pir M.S., Yenisert F., Woerz F., Hoffmann F., Altunkaynak B., Pir B., Boldt K., Karaman A., Cakiroglu M., Oner S.S., Cao Y., Ueffing M, & Kaplan O.I. (2023). WDR31 displays functional redundancy with GTPase-activating proteins (GAPs) ELMOD and RP2 in regulating IFT complex and recruiting the BBSome to cilium. Life Science Alliance, 6(8), e202201844.

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Generating Transgenic C. elegans Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

To generate transgenic lines for localization, rescue experiments, and expression patterns, we generated the following transgenic animals via microinjections. The rol-6 plasmid (50 ng/ μl plasmid pRF4) was co-injected as the co-transformation marker. In brief, the plasmids were delivered by microinjections into the gonads of 1-day adult worms.
Worms were initially transferred onto a 2.5% agarose pad prior (Halocarbon oil, Sigma: 9002-83-9), followed by microinjection. The microinjection was done using a Zeiss Axio Vert.A1 inverted microscope with DIC optics coupled with a Narishige Micromanipulator MMO-4. We next manually inspected the plates to find successful transgenics animals. Fluorescent transgenes for ciliary proteins: PHX1180, wdr-31(syb1180 [wdr-31(T05A8.5 )::GFP]); PHX1328, wdr-31(syb1328[wdr-31( R261-de) )::GFP]) (Disease mimicking variant); OIK1042 N2;turEx21 [arl-13p::wdr-31 (T05A8.5) ; unc122p::dsRed] . All PHX strains were generated using CRISPR/Cas9 by Sunybiotech. The list of extensive transgenic and mutant strains was provided in Table S2.

Cevik S., AlAbdi L., Peng X., Beyer T., Zorluer A., Pir M.S., Yenisert F., Shaheen R., Woerz F., Hoffmann F., Altunkaynak B., Pir B., Boldt K., Karaman A., Çakıroğlu M., Oner S.S., Cao Y., Ueffing M., Alkuraya F.S., & Kaplan O.I. (2021). WDR31 is a novel ciliopathy protein displaying functional redundancy with GTPase-activating proteins ELMOD and RP2 in recruiting BBSome to cilium.

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