Emx 10 12

The solubility of purified P. marneffei pigment from FRR2161 and GXMU110608 strains and synthetic DOPA-melanin (2 mg) were tested with distilled water, hot 1.0 M NaOH solution and common organic solvents (methanol, absolute ethanol, propanol, acetone, acetonitrile, iso-amyl alcohol, and chloroform). Reactions with oxidizing agents such as 30% hydrogen peroxide (H₂O₂) were determined. Purified P. marneffei melanin was subjected to various analyses such as UV-visible absorption (Varian Cary50), Fourier transform infrared (FT-IR) spectra (Perkin Elmer Spectrum100) and electron-paramagnetic resonance (EPR) (Bruker EMX-10/12). The UV absorption was performed using 40 μg/ml P. marneffei melanin dissolved in sodium hydroxide scanning at approximately 200–800 nm wavelength. The dried 2 mg P. marneffei melanin was mixed with 200 mg potassium bromide by grinding in an agate mortar for 5 min, and the sample was examined by infrared spectral scanning at approximately 4000–400 cm^-1. The various instrumental parameters of EPR were set at 100 kHZ modulation frequency, 1.0 G modulation amplitude, 5.0 mW microwave power, 9.75 GHZ microwave frequency, 3475.0 G center magnetic field strength, 100.0 G scan width and 83.89 s scan time.

Elemental analyses for C, H, N, and S were performed on Perkin-Elmer 240C analyzer. FT-IR data were recorded on Vector27 Bruker Spectrophotometer with KBr pellets in the 4000–400 cm⁻¹ region. TGA data were obtained on an STA 449C thermal analysis system with a heating rate of 10 °C min⁻¹ under N₂ atmosphere. The PXRD were collected with a scan speed of 0.1 s deg⁻¹ on a Bruker Advance D8 (40 kV, 40 mA) diffractometer with Cu radiation (λ = 1.54056 Å) at room temperature. Calculated PXRD patterns were generated using Mercury 3.0. Magnetic susceptibility measurements were performed using a Quantum Design SQUID VSM magnetometer on microcrystalline samples for all compounds. EPR spectra were obtained by using a Bruker EMX-10/12 variable-temperature apparatus at 110 K. Gas sorption measurements were conducted using a Micrometritics ASAP 2020 system. See Supplementary Methods for details.

The in situ EPR experimental data were obtained over a commercial EPR spectrometer Bruker EMX-10/12 at a X-band (9 GHz). Samples were prepared by mixing the catalyst in a glass tube with 0.5 mL solution of CH₃CN/TEOA (20:1). The sample was degassed by N₂ and then sealed. Then the glass tube was fixed into the EPR resonator. When needed, the CO₂ were introduced into the sample. The experiments were performed under visible-light irradiation.

The crystalline phase pattern of B-nZVI/BC was analyzed with X-ray diffraction using Cu K_α radiation (XRD, X’TRA, Geneva, Swiss). The surface morphology and the elemental composition of the composite were measured by scanning electron microscopy (SEM, Hitachi S-4800, Tokyo, Japan) and transmission electron microscopy (TEM, FEI Tecnai G2 spirit, Eindhoven, Holland) equipped with energy dispersive spectroscopy (EDS, Bruker, QUANTAX 400, Saarland, Germany). The Brunauer-Emmett-Teller specific surface areas (SA_BET) of B-nZVI/BC were observed using the N₂ adsorption method (Micromeritics, ASAP 2020 M + C, Norcross, GA, USA). The surface compositions and the valence states of the composites were determined by X-ray photoelectron spectroscopy (XPS, Shimadzu, AXIS UltraDL, Kyoto, Japan). Reactive free radicals were recorded through an electron paramagnetic resonance (EPR) spectrometer (Bruker, EMX-10/12, Saarland, Germany).

The temperature-dependent molar magnetic susceptibility of crystalline solid samples was measured on a Quantum Design SQUID-VSM magnetometer with an applied field of 5000 G or 2000 G. The magnetic susceptibility data were corrected for the diamagnetic contribution of the capsule and the sample holder. Molar susceptibility data were corrected for diamagnetic contribution of Pascal’s constants^{53 (link)}, The ESR measurements were obtained with a Bruker EMX-10/12 and EPR-plus X-band (9.4 GHz) digital EPR spectrometer with Bruker N₂-temperature controller. Spectral analysis and simulations were performed using the EasySpin program.