Hc pl apo 20x 0.75 imm corr cs2

To gain a better insight into the microstructure, some samples of material composition C1 were imaged with an inverted bright field light microscope (Axio Observer D1, Carl Zeiss, Oberkochen, Germany) equipped with an objective having 10x magnification and a numerical aperture of 0.25 (A-PLAN 10x/0.25, Carl Zeiss). Some samples of material composition C4 were also imaged using an inverted confocal laser scanning microscope (TCS SP8, Leica Microsystems, Mannheim, Germany) equipped with a multi-immersion objective with numerical aperture of 0.7 and 20x magnification (HC PL APO, 20x/0.75 IMM CORR CS2, Leica Microsystems). The confocal microscope contains two solid-state lasers with excitation wavelengths of 488 nm and 552 nm to excite two different fluorescent dyes as shown in the example images of Fig. 1b-d. The materials in this image were prepared and imaged as described by Bossler and Koos.8 (link) For the samples C4 imaged using confocal microscopy, small amounts of the fluorescent dye rhodamine B isothiocyanate (Sigma-Aldrich, Steinheim, Germany) were added to the aqueous glycerol before preparing the sample. The dye is excited by the 552 nm laser and the signal emitted in a wavelength interval of 560-660 nm was recorded.