Amazon speed ion trap mass spectrometer

All mass spectra were acquired using an ESI ion source Bruker Daltonik amaZon speed ion trap mass spectrometer (Bruker Daltonik, Bremen, Germany). The ion source was operated in positive mode with nitrogen as the drying gas, with a flow rate of 5 L/min at 180 °C, nebulizer pressure of 7.5 psi, capillary voltage of 4.5 kV, and end plate offset of 0.5 kV. Optimum ion transfer was achieved by automatically running the system smart parameter setting in order to optimize the ion transfer for the desired m/z value. Charge control of the ion trap was activated with a target value of 200,000 and a maximum accumulation time of 10 ms. Samples of 1 mM azathioprine in methanole were injected directly into the mass spectrometer at a flow rate of 180 μL h⁻¹. Mass spectra were recorded for m/z values between 50 and 380 at 5200 amu/s in maximum resolution scan mode and ten scans were averaged into one mass spectrum.

The mass spectra were recorded using an ESI ion source Bruker Daltonik amaZon speed ion trap mass spectrometer (Bruker Daltonik, Bremen, Germany). The ion source was operated in positive mode with nitrogen as the drying gas, with a flow rate of 5 L min⁻¹ at 180 °C, a nebulizer pressure of 7.5 psi, a capillary voltage of 4.5 kV, and an end-plate offset of 0.5 kV. Optimum ion transfer was achieved by automatically running the system smart parameter setting in order to optimize the ion transfer for the desired m/z value. Charge control of the ion trap was activated, with a target value of 200,000 and a maximum accumulation time of 10 ms. Samples of 100 µM BTZ in methanol were injected directly into the mass spectrometer at a flow rate of 180 µL h⁻¹. Mass spectra were recorded for m/z values between 50 and 750 at 5200 a.m.u. s⁻¹ in maximum-resolution scan mode, and 10 scans were averaged into one mass spectrum.
The acidic digestion procedure of DNA before and after incubation with BTZ consist in the mechanical removal of the DNA film from the electrode surface. The DNA film, weighing about 350 µg (10 µL drop containing 35 mg mL⁻¹ of dsDNA), was treated with HClO₄ 9 M during 10 min. Then, the digestion was stopped by neutralization with NaOH 9 M. All digested DNA samples were diluted with purified water and methanol and subjected to MS spectrometry.

The EYELA multi-shaker MMS-type and centrifugal evaporators were obtained from Tokyo Rika Kikai (Tokyo, Japan) and Sakuma Seisakusho Co., Ltd. (Tokyo, Japan). A SpectraMax iD5 microplate reader (Molecular Devices, San Jose, CA, USA) was used for the in vitro assays. A Nexera X2 HPLC/UHPLC system (Shimadzu, Kyoto, Japan) was used for quantitative chromatographic analysis. A Bruker amaZon Speed Ion-Trap Mass Spectrometer (Billerica, MA, USA) was used for chemical identification. For the in situ soil analysis, a soil moisture sensor kit SM150T (Delta-T Devices, Cambridge, UK) and a Yamanaka-type soil hardness tester (Fujiwara Seisakusho, Tokyo, Japan) were used. Soil macronutrients were analyzed using an EW-THA1J soil analyzer (Air Water Biodesign, Osaka, Japan).