Mice were placed in individual fear conditioning chambers (Med Associates). During the acquisition phase, mice were habituated to the chamber for 2 min, and then received three trains of a 30-s loud tone (90 dB) followed immediately by a 0.5-mA footshock for 2 s with a 1-min intertrain interval. Mice remained in the chamber for an additional 1 min before being returned to their home cages. For context-dependent fear conditioning, 24 hr later mice were returned to the chambers for 5 min without a tone or shock, and the amount of time spent freezing was determined. Freezing behavior was defined as no movement except for respiration. Cue-dependent fear conditioning was assessed 4 hr later. Mice were habituated in a novel environment scented with vanilla without any tone or shock for 3 min followed by a 3-min tone. The amount of time spent freezing during the 3-min tone period was determined. The following day, the animals’ response to shock was assessed by exposing the mice to increasing footshock intensities (0.05–0.45 mA).
Individual fear conditioning chambers
Manufactured by Med Associates
Individual fear conditioning chambers are specialized laboratory equipment used to study the behavioral and physiological responses of subjects to specific stimuli. These chambers provide a controlled environment for conducting experiments related to fear conditioning and related phenomena.
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2 protocols using individual fear conditioning chambers
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Fear Conditioning in Mice
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Fear Conditioning in Mice
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Mice were placed in individual fear conditioning chambers (Med Associates). During the acquisition phase, mice were habituated to the chamber for 2 min, and then received three trains of a 30-s loud tone (90 dB) followed immediately by a 0.5-mA footshock for 2 s with a 1-min intertrain interval. Mice remained in the chamber for an additional 1 min before being returned to their home cages. For context-dependent fear conditioning, 24 hr later mice were returned to the chambers for 5 min without a tone or shock, and the amount of time spent freezing was determined. Freezing behavior was defined as no movement except for respiration. Cue-dependent fear conditioning was assessed 4 hr later. Mice were habituated in a novel environment scented with vanilla without any tone or shock for 3 min followed by a 3-min tone. The amount of time spent freezing during the 3-min tone period was determined. The following day, the animals’ response to shock was assessed by exposing the mice to increasing footshock intensities (0.05–0.45 mA).
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