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Plpcx vector dna

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The PLPCX vector DNA is a plasmid DNA designed for gene expression in mammalian cells. It contains elements necessary for DNA replication and selection in bacterial and mammalian cells.

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Lab products found in correlation

Pfu ultra dna polymerase, by Agilent Technologies (2 mentions)

Close spelling variants of this product

PLPCX (17 citations) PLPCX vector (9 citations)

2 protocols using plpcx vector dna

1

Plasmid Cloning and Mutagenesis

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Plasmids pNL43/XmaI, pUC19.2LTR, pNLX.Luc.R- [31 (link)], pCG-VSV-G, pCG-gagpol, pIRES2-eGFP, pIRES2-eGFP-LEDGF-HA [10 (link)], pKBIN6Hthr [48 (link)], and pFT-1-LEDGF [19 (link)] were previously described. Plasmid pLPCX-HA-LEDGF/p75 was built by inserting PCR-amplified HA-LEDGF/p75 into pLPCX vector DNA (Clontech Laboratories, Inc., Mountain View, California). Mutations were introduced by PCR using Pfu Ultra DNA polymerase (Agilent Technologies, Inc., Santa Clara, California), and plasmids were sequenced to verify the presence of mutations and absence of unwanted secondary changes.
Wang H., Shun M.C., Li X., Di Nunzio F., Hare S., Cherepanov P, & Engelman A. (2014). Efficient transduction of LEDGF/p75 mutant cells by complementary gain-of-function HIV-1 integrase mutant viruses. Molecular Therapy. Methods & Clinical Development, 1, 2-.
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2

Plasmid Construction and Mutagenesis

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Plasmids pNL43/XmaI, pUC19.2LTR, pNLX.Luc.R−,31 (link) pCG-VSV-G, pCG-gagpol, pIRES2-eGFP, pIRES2-eGFP-LEDGF-HA,10 (link) pKBIN6Hthr,48 (link) and pFT-1-LEDGF19 (link) were previously described. Plasmid pLPCX-HA-LEDGF/p75 was built by inserting PCR-amplified HA-LEDGF/p75 into pLPCX vector DNA (Clontech Laboratories, Mountain View, CA). Mutations were introduced by PCR using Pfu Ultra DNA polymerase (Agilent Technologies, Santa Clara, CA), and plasmids were sequenced to verify the presence of mutations and absence of unwanted secondary changes.
Wang H., Shun M.C., Li X., Di Nunzio F., Hare S., Cherepanov P, & Engelman A. (2014). Efficient transduction of LEDGF/p75 mutant cells by complementary gain-of-function HIV-1 integrase mutant viruses. Molecular Therapy. Methods & Clinical Development, 1, 2-.
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