A deep sclerectomy was performed on the left eye of 16 rabbits under an operating microscope. Briefly, the dorsal bulbar conjunctiva was incised through the conjunctival sac to expose the sclera. A 4 × 4mm square scleral incision was made 3 mm posterior to the limbus. Dissection was continued until reaching the choroid. The defect thus created was filled either by a 4 × 4 mm square of lhUC-AM with epithelial side facing up (n = 12) or by replacing the freshly excised sclera (n = 4). Grafts were sutured using 8 to 10 separated stitches of 9/0 PGA filament (Vicryl®, Ethicon, Johnson and Johnson, Issy les Moulineaux, France). Bulbar conjunctival incision was then closed with continuous suture, using 6-0 PGA polyfilament (Vicryl®, Ethicon, Johnson and Johnson, Issy les Moulineaux, France).
Vicryl
Manufactured by Johnson & Johnson
Sourced in United States, Germany, Brazil, France, Australia, Italy
Vicryl is a sterile, absorbable surgical suture material composed of a copolymer of glycolic acid and lactic acid. It is designed for use in general soft tissue approximation and/or ligation, including use in ophthalmic procedures.
Automatically generated - may contain errors
Lab products found in correlation
Prolene, by Johnson & Johnson (7 mentions)
Monocryl, by Johnson & Johnson (6 mentions)
Ethibond, by Johnson & Johnson (5 mentions)
Ethilon, by Johnson & Johnson (4 mentions)
Vicryl plus, by Johnson & Johnson (4 mentions)
Rompun, by Bayer (3 mentions)
Ethibond excel, by Johnson & Johnson (3 mentions)
Pds 2, by Johnson & Johnson (3 mentions)
Monosyn, by B. Braun (3 mentions)
Formalin, by Merck Group (2 mentions)
Ketamine xylazine, by Merck Group (2 mentions)
Proparacaine hydrochloride ophthalmic solution, by Bausch & Lomb (2 mentions)
Xylazine, by Bayer (2 mentions)
Neutral buffered formalin, by Merck Group (2 mentions)
Zoletil 50, by Virbac (2 mentions)
Ketamine, by Pfizer (2 mentions)
Mersilk, by Johnson & Johnson (2 mentions)
Endobutton, by Smith & Nephew (2 mentions)
Dermabond, by Johnson & Johnson (2 mentions)
Ketalar, by Pfizer (1 mentions)
157 protocols using vicryl
1
Scleral Defect Repair in Rabbits
2
Open Castration Technique in Boars
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An open castration technique was used for castration of the boars and the scrotal incision was closed to reduce interference by fellow animals in the post-operative period. Following preparation of the skin for aseptic surgery, the non-dependent testis was digitally immobilised in the scrotum. A single longitudinal incision, parallel to the median raphe, was made through the scrotal skin, subcutaneous tissue and tunica vaginalis. The testis, epididymis and part of the spermatic cord were identified and isolated. The spermatic cord and vessels were ligated. The spermatic cord was incised and the testis, epididymis and distal part of the spermatic cord were removed. The tunica vaginalis was closed with a continuous suture pattern with absorbable suture material (Vicryl, Ethicon, Inc., Johnson & Johnson Medical Pty. Ltd., North Ryde, Australia). The scrotal skin was closed with a continuous intradermal suture pattern using absorbable suture material (Vicryl, Ethicon, Inc., Johnson & Johnson Medical Pty. Ltd., North Ryde, Australia).
3
Saphenous Nerve Ligation for Neuropathic Pain
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To model focal neuropathic pain, the saphenous nerve of the right hindlimb was ligated ten days prior to imaging14 (link). Mice were anesthetized with isoflurane (5% induction, 2% maintenance in 1 L/min O2) and administered buprenorphine (SC, 0.05 ml, 0.3 mg/ml) for analgesia. Body temperature was maintained at 37 °C with a thermometer feedback controlled heatpad. The anterior aspect of the left hindlimb was shaved and disinfected with betadine and alcohol (3x alternating), and an 8 mm incision was made overlying the saphenous triad. Subcutaneous and connective tissue was blunt dissected to isolate the saphenous nerve and 8-0 braided suture (Vicryl, Ethicon Inc., USA) was tied loosely around the nerve at 1 mm intervals. Sham animals underwent the same incision and dissection surgical procedure but no ligation was performed. Subsequent to ligation, the primary incision was closed (5-0 Vicryl, Ethicon Inc., USA) and mice were recovered and returned to their homecage.
4
Surgical Repair of Achilles Tendon Rupture
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All ATRs were repaired during open procedures via a posteromedial longitudinal incision. Ruptured tendons were apposed using a core Kessler stitch with a No. 2 nonabsorbable braided suture5 (link) supplemented with 3-0 Vicryl (Ethicon) circumferential sutures. The paratenon was closed with 3-0 Vicryl sutures, and then the skin was closed with interrupted fine nylon mattress sutures (Ethicon). The limb was postoperatively immobilized in a full equinus cast.
5
Modified W-shaped Tongue Reduction Surgery
6
Bilateral Ovarian and Uterine Horn Resection in Rats
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Xylazine (Alfazyne, Alfasan, Amsterdam) was given at 10 mg/kg/i.m. for neuromuscular blockage and ketamine (Ketalar Flakon, Pfizer, Istanbul) was given at 50 mg/kg/i.m. for anesthesia in all surgeries. Next, the rat was placed in a supine position. The abdomen was shaved, and the skin was cleaned. A midline laparotomy was performed with a 2–3 cm vertical incision. After the surgical procedures, the abdomen was closed by suturing in layers.
All surgeries were performed by same surgeons (MCS, OLT, RTA, SKA);
All surgeries were performed by same surgeons (MCS, OLT, RTA, SKA);
In the first surgery, bilateral ovarian tissues were ligated with a 3.0 polyglactin 910 suture (Vicryl, Ethicon, USA) from the proximal and distal zones and resected.
In the second surgery, the left uterine horns of all rats (in all groups) were resected by ligation with a 3.0 polyglactin 910 suture (Vicryl, Ethicon, USA) from the proximal zone.
In the third surgery, the right uterine horns of all rats (in all groups) were resected by using the same procedure as that used in the previous surgery. The animals were sacrificed by decapitation.
7
Laparoscopic Myomectomy Technique Comparison
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All operations were performed by the same surgeon (BM). The first entry into the abdomen was made by direct trocar entry from the umbilicus or Lee Huang point according to the size of the fibroid, and a 10-mm laparoscope was placed in this port. The 30-degree optic was used in all cases to provide a wide viewing angle. Then, 5-mm accessory trocars were placed in the right and left lower quadrant and suprapubic area. Rumi II was used as the uterine manipulator. A Harmonic scalpel (Ethicon Endo-Surgery, Cincinnati, OH, USA) was used to create the incision in the uterine serosa. A Vicryl (Ethicon, Somerville, NJ, USA) 0 USP 40-mm ½ taper needle suture was used for the Vicryl group patients. A V-Loc 180 (Covidien, Mansfield, MA, USA) 0 USP 37-mm ½ taper needle suture was used for the barbed suture group patients. One, two, or three layers were closed continuously depending on the depth of the fibroid inside the myometrium. All fibroids were morcellated with a Rotocut G1 electronic morcellator device (Karl Storz, Tuttlingen, Germany) and then removed from the abdomen.
Hemoglobin was measured the day before the operation and at the eighth postoperative hour. A cut-off value of <7 g/dL was determined for blood transfusion. Transfusion was performed in symptomatic patients with higher values.
Hemoglobin was measured the day before the operation and at the eighth postoperative hour. A cut-off value of <7 g/dL was determined for blood transfusion. Transfusion was performed in symptomatic patients with higher values.
8
Tracheal Resection and Anastomosis Technique
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The surgical principles of tracheal resection and anastomosis (TR/A) have been already described (8 (link)). In our experience, after the two 2/0 polyglactin (Vicryl; Ethicon, Inc., Somerville, NJ, USA) traction sutures were placed in the lower tracheal tract and to the larynx, respectively, the posterior anastomosis was carried out first through a 4/0 polydioxanone (PDS; Ethicon, Inc., Somerville, NJ, USA) continuous suture on the membranous pars; soon after, the latero-anterior cartilaginous pars was sutured with interrupted 3/0 Vicryl stitches. The traction sutures were then tied together to release tensions on the anastomosis and pretracheal muscle flaps are used to cover the anastomosis.
9
Sciatic Nerve Compression Injury Model
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All surgical procedures were performed under general anesthesia with 3% isoflurane. Routinely, Buprenorphine (Temgesic ® , 0.3 mg/ml, 0.05 mg/kg; Indivior AG, Baar, Switzerland) was administered before and after the surgery. The left sciatic nerve was approached dorsally using a gentle spreading technique of the gluteus muscle. The sciatic nerve was compressed with a titanium hemostatic clip (Vitalitec small clip; Péters surgical, Bobigny, France) at 10 mm proximally from the sciatic bifurcation point into tibial and peroneal branches. For this, a custom-made instrument that was preset to leave only the maximum lumen size of 250 μm after complete compression was used [Citation22]. The compressing clip was left undisturbed on the sciatic nerve until the end of the experimentation. Muscles and fascia layers were closed with a single resorbable stitch (5/0 Vicryl, Ethicon) and the skin was closed by a continuous running suture (5/0 Vicryl, Ethicon). For analgesia, the animals received subcutaneous injections of Meloxicam (Metacam ® , 2 mg/ml; Boehringer Ingelheim GmbH, Basel, Switzerland) 2 mg/kg bodyweight postoperatively, and then 1 mg/kg bodyweight every 24 h for 2 days. Furthermore, Paracetamol (Becetamol ® , 100 mg/ml; Gebro Pharma AG, Liestal, Switzerland) 50 mg/kg bodyweight/day was added to the drinking water for 7 days.
10
Surgical Simulation in Rats
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