The B16F10 murine melanoma cell line was purchased from the Korean cell line bank (KCLB, Seoul, Republic of Korea). The B16F10 cells were cultured in a DMEM medium (with 10% fetal bovine serum, 1% antibiotic antimycotic, and 1% penicillin-streptomycin), and the cell culture was maintained in a humidified CO2 cell incubator.
B16f10 murine melanoma cell line
Manufactured by Korean Cell Line Bank
The B16F10 murine melanoma cell line is a commonly used cell line derived from a C57BL/6 mouse melanoma. It is a well-established model for studying melanoma biology and tumor progression.
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3 protocols using b16f10 murine melanoma cell line
1
Culturing B16F10 Murine Melanoma Cells
2
Culturing Murine Melanoma Cells
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The B16F10 murine melanoma cell line was purchased from the Korean Cell Line Bank (KCLB) (Seoul, Korea). The cells were cultured in Dulbecco’s modified Eagle’s medium with 10% FBS and 100 units/ml penicillin plus 100 mg/ml streptomycin at 37 °C with 5% CO2 according to the KCLB guideline. After the cells reached 70–80% confluence, they were subcultured using 0.25% trypsin/EDTA (Thermo Fisher Scientific, Waltham, MA, USA).
3
B16F10 Murine Melanoma Cell Culture
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B16F10 murine melanoma cell line was purchased from the Korean cell line bank (KCLB, Seoul, Republic of Korea). The B16F10 cells were cultured in complete DMEM medium containing 10% FBS, 1% antibiotic antimycotic and 1% penicillin-streptomycin, and the culture was maintained in a humidified incubator (5% CO2) at 37ºC.
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