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Digital sight ds ri2 high definition colour camera

Manufactured by Nikon
Sourced in Japan

The Digital sight DS-Ri2 is a high definition color camera designed for use in various laboratory applications. It features a CMOS image sensor capable of capturing images and videos in high resolution. The camera provides accurate color representation and can be used for a range of imaging tasks in research and analytical settings.

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8 protocols using digital sight ds ri2 high definition colour camera

1

Identification of Dendrostoma Species

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The identification of Dendrostoma spp. was based on morphological features observed on the natural substrates. Cross-sections for ascomata and conidiomata from tree barks were prepared by hand using a double-edged blade under a dissecting microscope. At least 10 conidiomata/ascomata, 10 asci and 50 conidia/ascospores were measured to calculate the mean size and standard deviation. Measurements are reported as maxima and minima in parentheses and the range representing the mean plus and minus the standard deviation of the number of measurements is given in parentheses (Voglmayr et al. 2017 (link)). Microscopy photographs were captured with a Nikon Eclipse 80i compound microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast illumination. Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ). Cultural characteristics were recorded for isolates incubated on PDA in the dark at 25 °C.
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2

Taxonomic Study of Cytospora on Castanea mollissima

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Observation and description of Cytospora species from Castaneamollissima was based on fruiting bodies formed on tree barks. Ascomata and conidiomata from tree barks were sectioned by hand using a double-edged blade and strctures were observed under a dissecting microscope. At least 10 conidiostromata/ascostromata, 10 asci and 50 conidia/ascospores were measured to calculate the mean size and standard deviation. Measurements are reported as maximum and minimum in parentheses and the range representing the mean plus and minus the standard deviation of the number of measurements is given in parentheses (Voglmayr et al. 2017 (link)). Microscopy photographs were captured with a Nikon Eclipse 80i compound microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast illumination. Introduction of the new species, based on molecular data, follow the recommendations of Jeewon and Hyde (2016) (link).
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3

Fungal Species Identification on Rhus chinensis

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Recognition and identification of the fungal species on Rhuschinensis was based on fruiting bodies formed on the bark. Ascomata and conidiomata were sectioned by hand using a double-edged blade, and microscopic structures were observed under a dissecting microscope. At least 10 conidiomata/ascomata, 10 asci, and 50 conidia/ascospores were measured to calculate mean and standard deviation. Measurements are reported as maxima and minima in parentheses and the range representing the mean plus and minus the standard deviation of the number of measurements given in parentheses (Voglmayr et al. 2017 (link)). Microscopy photographs were captured with a Nikon Eclipse 80i compound microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast illumination. Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ).
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4

Morphological Identification of Fungal Species

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Species identification was based on the morphological characters of apothecia and conidiomata produced on natural substrates. Cross-sections were prepared manually using a double-edged blade under a Leica stereomicroscope (M205 FA). Photomicrographs were captured with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high-definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software, NIS-Elements D Package 3.00. Measurements of ascospores and conidia are reported as the maximum and minimum in parentheses and the range representing the mean ± standard deviation of the number of measurements is given in parentheses. Cultural characteristics of isolates incubated on MEA in the dark at 25 °C were recorded.
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5

Fungal Identification and Characterization

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Species identification was based on morphological features of the ascomata or conidiomata produced on infected plant tissues and micromorphology, supplemented by cultural characteristics. Cross-sections were prepared by hand using a double-edge blade under a dissecting microscope. At least 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size and standard deviation (SD). Microscopic photographs were captured with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ). Colony diameters were measured, and the colony colours described after 3 wk according to the colour charts of Rayner (1970) .
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6

Fungal Morphological Characterization

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Descriptions were performed based on morphological features of the ascomata or conidiomata from infected host materials. The macro-morphological photographs were captured using a Leica stereomicroscope (M205 FA) (structure and size of stromata, structure and size of ectostromatic disc and ostioles). Micro-morphological observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. Over 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size/length and respective standard deviations (SD). Colony diameters were measured and the colony features were described using the color charts of Rayner (1970) . Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ).
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7

Morphological Identification of Fungal Species

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Species identification was based on morphological features of the ascomata or conidiomata from infected host materials and micromorphology, supplemented by cultural characteristics. Microscopic photographs (structure and size of stromata; structure and size of ectostromatic disc and ostioles) were captured using a Leica stereomicroscope (M205 FA) (Leica Microsystems, Wetzlar, Germany). Microscopic observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope (Nikon Corporation, Tokyo, Japan), equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination. The Nikon software NIS-Elements D Package v. 3.00, Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. More than 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured by Nikon software NIS-Elements D Package v. 3.00 to calculate the mean size/length and respective standard deviations (SD). Colony diameters were measured and the colony features were described using the colour charts of Rayner (1970) .
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8

Fungal Species Identification Protocol

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Species identification was based on morphological features of the ascomata or conidiomata produced on infected plant tissues and micromorphology, supplemented by cultural characteristics. Microscopic photographs were captured using a Leica stereomicroscope (M205 FA) (Leica Microsystems, Wetzlar, Germany) (structure and size of stromata; structure and size of ectostromatic disc and ostioles). Microscopicl observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope (Nikon Corporation, Tokyo, Japan) equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. More than 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size/length and respective standard deviations (SD). Colony diameters were measured and the colony features were described using the colour charts of Rayner (1970) . Nomenclatural novelties and descriptions were deposited in MycoBank (www.mycobank.org; Crous et al. 2004) .
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