The PTX was purchased from Indústria Farmin e Comércio Ltd.a (Guarulhos, São Paulo, Brazil) in the commercial form of Evotaxel®. The PTX was prepared from a concentration of 6 mg/mL to a dose of 2 mg/kg through dilution with solution for injections. The MTDZ (Scheme 1 ) was synthesized and characterized in the Catalysis and Biocatalysis Laboratory at the Federal University of Grande Dourados as previously described by Santos et al. [7 (link)]. The MTDZ’s purity, which stood at 99%, was assessed through column chromatography using exclusively hexane. The 1H and 13C NMR spectra were captured in CDCl3 using a Bruker spectrometer, operating at frequencies of 300 MHz and 75 MHz, respectively. For experimentation, the MTDZ was dissolved in canola oil, while PTX was dissolved in a 5% glucose solution.
The other drugs (naloxone, capsaicin, methylene blue, L−arginine hydrochloryde (L−arginine) and ω−nitro−L−arginine (L−NOARG), ketanserin, pindolol, WAY100635, acetic acid P.A., monosodium glutamate (MSG), and MK−801) were obtained commercially from Sigma−Aldrich (St. Louis, MO, USA). ketanserin, WAY100635, MK−801, L−arginine, L−NOARG, methylene blue, capsaicin, MSG, acetic acid, and naloxone were dissolved in isotonic saline solution; pindolol was dissolved in Tween 80 (10%).
The other drugs (naloxone, capsaicin, methylene blue, L−arginine hydrochloryde (L−arginine) and ω−nitro−L−arginine (L−NOARG), ketanserin, pindolol, WAY100635, acetic acid P.A., monosodium glutamate (MSG), and MK−801) were obtained commercially from Sigma−Aldrich (St. Louis, MO, USA). ketanserin, WAY100635, MK−801, L−arginine, L−NOARG, methylene blue, capsaicin, MSG, acetic acid, and naloxone were dissolved in isotonic saline solution; pindolol was dissolved in Tween 80 (10%).