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Ros glo h2o2 kit

Manufactured by Promega
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The ROS-Glo H2O2 Kit is a luminescent-based assay for the detection and quantification of hydrogen peroxide (H2O2) in biological samples.

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Lab products found in correlation

Infinite 200 pro nanoquant, by Tecan (2 mentions) Synergy h4 microplate reader, by Agilent Technologies (1 mentions) Dcfda h2dcfda cellular ros assay kit, by Abcam (1 mentions) Clariostar microplate reader, by BMG Labtech (1 mentions)

Close spelling variants of this product

ROS-GloTM H2O2 assay kit ROS-Glo H2O2 ROS-Glo kit ROS-Glo

6 protocols using ros glo h2o2 kit

1

Viral Reactivation and H2O2 Measurement

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iSLK.219 and AGS-EBV cells were transfected with NS and TBRG4 siRNA, and then viral lytic reactivation was induced with Dox and TPA, respectively. The production of H2O2 was measured by using a ROS-Glo H2O2 kit (Promega) according to the manufacturer’s protocol. The assay is based on a luminescent signal generated by a chemical reactivation of H2O2 and its substrate. The luminescence was measured using a microplate reader. The fold change was calculated and normalized to NS-treated cells at 0 h post reactivation.
Zhang H., Wong J.P., Ni G., Cano P., Dittmer D.P, & Damania B. (2022). Mitochondrial protein, TBRG4, modulates KSHV and EBV reactivation from latency. PLOS Pathogens, 18(11), e1010990.
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2

ROS-Glo Assay for HeLa Cells

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5 × 103 HeLa cells/well were seeded in complete medium on 96 well-plates 24 h before the treatment with DBI. HeLa cells were washed (2 times) with live cell imaging solution (Molecular Probes™, Ref. No.: A14291DJ), then treated with DBI (1 μM) or DMSO (0.02% v/v) dissolved in live cell imaging solution and incubated for 90 min at 37°C in 5% CO2. When required, the cells were irradiated with blue light using a LED light cube (Ex: 470/22 nm) operating at 30 mW cm−2 for 4 min. After irradiation, ROS-Glo™ H2O2 kit (Promega) was used according to the manufacturer's instructions and the luminescence was recorded on a Synergy H4 microplate reader (Biotek).
Deiana M., Andrés Castán J.M., Josse P., Kahsay A., Sánchez D.P., Morice K., Gillet N., Ravindranath R., Patel A.K., Sengupta P., Obi I., Rodriguez-Marquez E., Khrouz L., Dumont E., Abad Galán L., Allain M., Walker B., Ahn H.S., Maury O., Blanchard P., Le Bahers T., Öhlund D., von Hofsten J., Monnereau C., Cabanetos C, & Sabouri N. (2023). A new G-quadruplex-specific photosensitizer inducing genome instability in cancer cells by triggering oxidative DNA damage and impeding replication fork progression. Nucleic Acids Research, 51(12), 6264-6285.
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3

ROS Measurement in Treated Cells

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Cells were seeded on 96-well white-walled plates (104 cells/well) and incubated overnight for attachment. The following day, cells were treated as indicated, and after 48 hours ROS were measured using the ROS-Glo H2O2 kit (Promega). Luminescence was measured using a plate-reading luminometer (TECAN, Infinite 200 PRO Nano Quant) and the resulting data were normalized to untreated cells at each time point.
Noronha A., Belugali Nataraj N., Lee J.S., Zhitomirsky B., Oren Y., Oster S., Lindzen M., Mukherjee S., Will R., Ghosh S., Simoni-Nieves A., Verma A., Chatterjee R., Borgoni S., Robinson W., Sinha S., Brandis A., Kerr D.L., Wu W., Sekar A., Giri S., Chung Y., Drago-Garcia D., Danysh B.P., Lauriola M., Fiorentino M., Ardizzoni A., Oren M., Blakely C.M., Ezike J., Wiemann S., Parida L., Bivona T.G., Aqeilan R.I., Brugge J.S., Regev A., Getz G., Ruppin E, & Yarden Y. (2022). AXL and Error-Prone DNA Replication Confer Drug Resistance and Offer Strategies to Treat EGFR-Mutant Lung Cancer. Cancer Discovery, 12(11), 2666-2683.
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4

ROS Measurement in Treated Cells

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Cells were seeded on 96-well white-walled plates (104 cells/well) and incubated overnight for attachment. The following day, cells were treated as indicated and after 48 hours ROS were measured using the ROS-Glo™ H2O2 kit (Promega). Luminescence was measured using a plate-reading luminometer (TECAN, Infinite ® 200 PRO Nano Quant) and the resulting data were normalized to untreated cells at each time point.
Noronha A., Belugali Nataraj N., Lee J.S., Zhitomirsky B., Oren Y., Oster S., Lindzen M., Mukherjee S., Will R., Ghosh S., Simoni-Nieves A., Verma A., Chatterjee R., Borgoni S., Robinson W., Sinha S., Brandis A., Kerr D.L., Wu W., Sekar A., Giri S., Chung Y., Drago-Garcia D., Danysh B.P., Lauriola M., Fiorentino M., Ardizzoni A., Oren M., Blakely C.M., Ezike J., Wiemann S., Parida L., Bivona T.G., Aqeilan R.I., Brugge J.S., Regev A., Getz G., Ruppin E, & Yarden Y. (2022). AXL and Error-Prone DNA Replication Confer Drug Resistance and Offer Strategies to Treat EGFR-Mutant Lung Cancer. Cancer Discovery, 12(11), 2666-2683.
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5

Measuring Oxidative Stress via H2O2 Kit

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Levels of H2O2 were determined via ROS-Glo H2O2 Kit (Promega). Cells were seeded in 96-well plates at 1 × 104 cells/well and cultured overnight until reaching ∼80% confluency. Expression of GFP (control) or Tuna was achieved as described above. Cells were exposed to 50 µM menadione for 2 h to induce oxidative stress as a positive control. Each condition was done in triplicate. Transfected, control, and menadione treated cells were analyzed at 24 h, and all experiments were repeated 3 times.
Simmers M.D., Jima D.D., Tsuji Y, & Cowley M. (2023). LncRNA Tuna is activated in cadmium-induced placental insufficiency and drives the NRF2-mediated oxidative stress response. Frontiers in Cell and Developmental Biology, 11, 1151108.
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6

Cellular Oxidative Stress Assay

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The assay was performed using the DCFDA/H2DCFDA – Cellular ROS Assay Kit (Abcam, #ab113851). Briefly, cells were transfected as described above and treated with a sublethal concentration of TMZ as previously determined, where specified. After 72 or 96 hours cells were stained with DCFDA solution according to the manufacturer's instructions and fluorescence was measured using the BMG Labtech CLARIOstar Microplate Reader at Ex/Em = 485/535 nm. Where indicated, the ROS‐Glo H2O2 kit (Promega, #G8820) was used following the manufacturer's instructions. Relative luminescence units (RLU) were determined using the GloMax Luminometer.
Vella V., Ditsiou A., Chalari A., Eravci M., Wooller S.K., Gagliano T., Bani C., Kerschbamer E., Karakostas C., Xu B., Zhang Y., Pearl F.M., Lopez G., Peng L., Stebbing J., Klinakis A, & Giamas G. (2024). Kinome‐Wide Synthetic Lethal Screen Identifies PANK4 as a Modulator of Temozolomide Resistance in Glioblastoma. Advanced Science, 11(15), 2306027.
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