Bx41 phase contrast darkfield microscope

Manufactured by Olympus

Sourced in Japan

The BX41 Phase Contrast & Darkfield Microscope is a laboratory equipment designed for optical microscopy. It provides phase contrast and darkfield illumination techniques to enhance the visibility of transparent or low-contrast specimens.

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Lab products found in correlation

Basescope duplex detection reagent kit, by Advanced Cell Diagnostics (2 mentions) Dp25 5mp color firewire camera, by Olympus (2 mentions) Nis elements software, by Nikon (1 mentions) Fisherbrand superfrost plus microscope slide, by Thermo Fisher Scientific (1 mentions) Superfrost plus microscope slide, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

BX41 phase-contrast microscope (12 citations) Phase-contrast microscope (486 citations) BX41 microscope (1002 citations)

4 protocols using bx41 phase contrast darkfield microscope

Histological Assessment of Bone Formation

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Each specimen collected at implant surgical stage was immediately fixed in 10% neutral buffered formalin, then, processed for two staining techniques; Haematoxylin & Eosin (H&E)^{52 (link)} and Masson–Goldner Trichrome^{53 (link)} stains for general examination of newly formed bone and detection of unmineralized bone respectively.
Slides were examined using Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Corporation, Japan), then, sections were captured using Olympus DP20 digital microscope camera (Olympus Corporation, Japan) for examination of histological slides, which was undertaken following a fully blinded approach within each study group.

Abd-Elhaleem Othman M.A., Zaky A.A., Eltayeb E.A, & Khalil N.M. (2024). A radiographic and histological study to compare red (650 nm) versus near infrared (810 nm) diode lasers photobiomodulation for alveolar socket preservation. Scientific Reports, 14, 6871.

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Fluorescence and Brightfield Microscopy

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Immunofluorescence images were captured by Nikon Eclipse 80i fluorescence microscope equipped with a CoolSnap MYO camera using Plan Apo VC 60× Oil DIC N2 objective lens. The images were acquired and processed using Nikon NIS-Elements software. Bright-field images were captured by an Olympus BX41 Phase Contrast & Darkfield Microscope using 100× oil objective lens. The metaphase images were acquired using DP2-BSW software. 5 μM scale bar was included on all the images.

Jiang Q., Foglizzo M., Morozov Y.I., Yang X., Datta A., Tian L., Thada V., Li W., Zeqiraj E, & Greenberg R.A. (2022). Autologous K63 deubiquitylation within the BRCA1-A complex licenses DNA damage recognition. The Journal of Cell Biology, 221(9), e202111050.

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Cryosectioning and in situ mRNA Detection in Exaiptasia

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Adult Exaiptasia were paralyzed in anesthetic solution (15% MgCl₂), rinsed in PBS, then embedded in Tissue-Tek O.C.T. Compound (Sakura Cat #4583) in cryomolds (Sakura Tissue-Tek® Cryomold®, Intermediate, Cat #4566) and flash frozen on dry ice and stored at −80°C. Cryostat sections (18–20μm) were adhered to Fisherbrand™ Superfrost™ Plus Microscope Slides (Fisher Scientific Cat #12–550-15) and flash frozen on dry ice and stored at −80°C until used for BaseScope. The BaseScope Duplex Detection Reagent Kit (Advanced Cell Diagnostics Cat #323800) and the manufacturer’s manual (BaseScope Duplex Detection Reagent User Manual, ACDBio) was followed to hybridize custom probes or positive control (Cat #700101) or negative control probes (Cat #700141) to targets in tissue cryosections and amplify signals. Samples were imaged on an Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Cat #BX41-PH-B) and images were acquired using the Olympus CellSens software and Olympus DP25 5MP Color Firewire Camera.

He L.S., Qi Y., Allard C.A., Valencia-Montoya W.A., Krueger S.P., Weir K., Seminara A, & Bellono N.W. (2023). Molecular tuning of sea anemone stinging. bioRxiv.

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Cryosectioning and BaseScope Analysis of Exaiptasia

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Adult Exaiptasia were paralyzed in anesthetic solution (15% MgCl₂), rinsed in PBS, then embedded in Tissue-Tek O.C.T. Compound (Sakura Cat #4583) in cryomolds (Sakura Tissue-Tek Cryomold, Intermediate, Cat #4566) and flash frozen on dry ice and stored at –80 °C. Cryostat sections (18–20 µm) were adhered to Fisherbrand Superfrost Plus Microscope Slides (Fisher Scientific Cat #12-550-15) and flash frozen on dry ice and stored at –80 °C until used for BaseScope. The BaseScope Duplex Detection Reagent Kit (Advanced Cell Diagnostics Cat #323800) and the manufacturer’s manual (BaseScope Duplex Detection Reagent User Manual, ACDBio) was followed to hybridize custom probes or positive control (Cat #700101) or negative control probes (Cat #700141) to targets in tissue cryosections and amplify signals. Samples were imaged on an Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Cat #BX41-PH-B) and images were acquired using the Olympus CellSens software and Olympus DP25 5MP Color Firewire Camera.

He L.S., Qi Y., Allard C.A., Valencia-Montoya W.A., Krueger S.P., Weir K., Seminara A, & Bellono N.W. (2023). Molecular tuning of sea anemone stinging. eLife, 12, RP88900.

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