For the transwell assay, 7 × 104 HGC27 cells or 5 × 104 AGS cells (without fetal bovine serum) were digested from 6-well plates and then inoculated into the upper chamber of 8 μm chambers (Corning, Corning, NY, USA). Then, 500 μL of medium containing 10% FBS was added to the lower chamber. After 24 h of incubation, the non-migrating cells in the upper chamber were gently wiped, and the migrating cells at the bottom of the chamber were fixed with 4% paraformaldehyde for 10 min, followed by staining with the crystalline violet solution for 8 min. The pictures were obtained and the cells were counted under a microscope.
8 μm chambers
Manufactured by Corning
Sourced in United States
The 8-μm chambers are a specialized lab equipment designed for specific applications. These chambers have an internal diameter of 8 micrometers, providing a precise and controlled environment for various experimental setups. The core function of these chambers is to facilitate the containment and manipulation of small-scale samples or materials, without making any claims about their intended use.
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Lab products found in correlation
9 protocols using 8 μm chambers
1
Transwell Migration Assay for HGC27 and AGS Cells
2
In Vitro Cell Invasion Assay
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Six-hole dishes with 8-μm chambers (Corning, United States) were applied for cellular invasion assessment (with Matrigel). Firstly, implant the transfected 97H and Huh7 cells on 6-hole dishes at 1 × 105 cell concentration. Add serum-free medium 200 μL to the upper chamber and 800 μL medium with 30% FBS to the lower chamber. Around 48 h later, fix the cells for 20 min in paraformaldehyde (4%) and stain for 15 min in crystal violet solution (0.1%). Randomly select 3 fields to calculate invasion cell number and to assess the cell invasion ability.
3
Lung Cancer Cell Migration and Invasion
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A wound healing assay was performed to analyze cell migration. The wound of lung cancer cells was created by a sterile pipette tip and continued incubating in medium for 24 h. The migrated distance of lung cancer cells under a microscope was captured. The relative migrated distance of cells is measured by the distance of cell migration/the distance measured at 0 h.
The Invasion assays was measured with a transwell system using 8 μm chambers (Corning). The transwell chambers were coated with 250 μg/mL BD Matrigel. Simply, 200 μL cells at a density of 2 × 105 cells/mL in FBS-free medium were then seeded into the top chambers, and 600 μL complete medium was added to the bottom chambers. After incubation at 37 °C for 48 h, the upper cells of transwell inserts were scraped and the lower invasive cells were stained with crystal violet and counted under a microscope.
The Invasion assays was measured with a transwell system using 8 μm chambers (Corning). The transwell chambers were coated with 250 μg/mL BD Matrigel. Simply, 200 μL cells at a density of 2 × 105 cells/mL in FBS-free medium were then seeded into the top chambers, and 600 μL complete medium was added to the bottom chambers. After incubation at 37 °C for 48 h, the upper cells of transwell inserts were scraped and the lower invasive cells were stained with crystal violet and counted under a microscope.
4
Transwell Migration Assay Protocol
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Transwell migration experiments were performed according to the manufacturer’s instructions for Corning 8-μm chambers. Briefly, 5 × 103 cells were placed in the upper compartment of a transwell chamber, with both chambers containing medium. Twenty-four hours later, the cells remaining in the upper compartment were removed using cotton swabs, while the cells that crossed the membrane were fixed and stained using crystal violet in methanol.
5
Lung Cancer Migration and Invasion Assays
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For cell migration, a wound healing assay was conducted. The lung cancer cells were wounded by scraping and incubated in the medium for 24 hours. The migrated distance of the lung cancer cells was visualized under a microscope. The relative migrated distance of the cells was measured by the distance of cell migration/the distance measured at hour 0.
The invasion of lung cancer cells was measured using a transwell assay with 8 μm chambers (Corning). The transwell chambers were coated with 100 μL BD Matrigel. A total of 200 μL of cells at a density of 2 × 105 cells/mL in the fetal bovine serum‐free medium was then seeded into the upper chambers, and the lower chambers were filled with 600 μL of the complete medium. After incubation for 48 hours, the invasive cells were stained with crystal violet and counted.
The invasion of lung cancer cells was measured using a transwell assay with 8 μm chambers (Corning). The transwell chambers were coated with 100 μL BD Matrigel. A total of 200 μL of cells at a density of 2 × 105 cells/mL in the fetal bovine serum‐free medium was then seeded into the upper chambers, and the lower chambers were filled with 600 μL of the complete medium. After incubation for 48 hours, the invasive cells were stained with crystal violet and counted.
6
Trans-Well Migration Assay Protocol
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Trans-Well assay was conducted using 8 μm chambers (Corning, Corning, NY, USA). 100 μL HEFs (cell density 2×105/well) with serum-free DMEM were transplanted into the upper chambers in a 24-well plate (WHB) following starvation treatment for 24 h and the lower chambers were respectively added in 500 μL of 10% DMEM with different treatment. After cells were cultured for 24 h, the upper chambers and complete medium in the lower chambers were removed. The unmigrated cells in upper layer were wiped with a cotton swab and 4% PFA was used to fix lower layer for 30 minutes. Then cells staining were conducted using 0.1% crystal violet for 30 minutes. Digital images were collected under a microscope.
7
Invasion Assay with LCL161 Stimulation
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A total of 2 × 104 cells were seeded onto 8-μm chambers (Corning, Wiesbaden, Germany), and the indicated concentration of LCL161 were added to both the lower and upper chambers for 24 h for stimulation. Cells on the upper chambers were scraped off using a cotton swab, and the cells in the lower chamber were fixed in 4% paraformaldehyde and stained with 0.4% crystal violet. The cells were quantified by inverted microscopy.
8
Cellular Migration and Invasion Assay
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Six-well plates with 8-μm chambers (Corning, USA) were used to assess cellular migration (without Matrigel) or invasion (with Matrigel). In brief, transfected HCT116 and SW620 cells were seeded in six-well plates at a concentration of 1 × 105 cells. In total, 200-μL serum-free medium was added to the upper chamber, and 600-μL of medium with 30% FBS was added to the lower chamber, and they were set aside for 48 h. The cells were then fixed with 4% paraformaldehyde for 30 min and stained with 0.1% crystal violet solution for 15 min. Five fields were randomly selected to calculate the area of migrating or invading cells.
9
Cellular Migration and Invasion Assay
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Six-well plates with 8-μm chambers (Corning, United States) were used to assess cellular migration (without Matrigel) or invasion (with Matrigel). Briefly, transfected HCT116 cells were seeded in 6-well plates at a concentration of 1 × 105 cells. Two hundred microliters of serum-free medium was added to the upper chamber, and six hundred microliters of medium with 30% fetal bovine serum was added to the lower chamber for 48 h. Then, the cells were fixed with 4% paraformaldehyde for 30 min and stained with 0.1% crystal violet solution for 15 min. Four fields were randomly selected to calculate the number of migrating or invading cells and to evaluate the ability of cell migration or invasion.
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