Image station 4000mm pro

Manufactured by Kodak

Sourced in United States, China

The Image Station 4000MM PRO is a high-performance lab equipment designed for digital image processing and analysis. It features advanced hardware and software capabilities to facilitate professional-grade image management and manipulation tasks.

Automatically generated - may contain errors

Lab products found in correlation

Ecl plus kit, by GE Healthcare (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Typhoon 9410 workstation, by Cytiva (1 mentions) Ecl kit, by PerkinElmer (1 mentions) Goat anti mouse secondary antibody, by Abcam (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Bicinchoninic acid protein assay kit, by Beyotime (1 mentions) Ripa lysis buffer, by BioTeke (1 mentions) Tissue extraction reagent, by Thermo Fisher Scientific (1 mentions) Anti chop, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti phospho eif2α, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Anti eif2α, by Cell Signaling Technology (1 mentions) Anti atf4, by Abcam (1 mentions) Semi dry trans blot cell, by Bio-Rad (1 mentions) Ndrg1, by Abcam (1 mentions) Lysis buffer, by Bio-Rad (1 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)

30 protocols using image station 4000mm pro

Quantitative Analysis of SDS-PAGE and Western Blots

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the quantification of the bands in SDS-PAGE and Western blotting two methods were used:

Samples from each age were loaded and compared to a standard curve on the same membrane. The fluorescent signal was detected with a Typhoon 9410 Workstation (Typhoon 9410, Amersham Biosciences, Piscataway, NJ, USA) and quantified with a commercially available image analysis program (ImageQuantTM TL, Amersham Biosciences, Piscataway, NJ, USA).

All samples were loaded on the same membrane. The fluorescent signal was detected with a Kodak scanner (Kodak Image Station 4000 MM PRO) and quantified with a commercially available image analysis program (Kodak MI SE, Carestream Health, Inc., Rochester, NY, USA).

Lunde L.K., Camassa L.M., Hoddevik E.H., Khan F.H., Ottersen O.P., Boldt H.B, & Amiry-Moghaddam M. (2014). Postnatal development of the molecular complex underlying astrocyte polarization. Brain Structure & Function, 220(4), 2087-2101.

+ Open protocol

+ Expand

ALA Metabolite Effects on RAW 264.7 and Peritoneal Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

RAW 264.7 cells seeded in 100 mm plates at a density of 5 × 10⁵ cells/well and then after 12 h pre-incubated with different concentrations (1, 50 and 100 μM) of either ALA metabolites or dexamethasone (10 μM) for 3 h. For Immunoblot analysis of primary cells, mouse peritoneal macrophages were isolated and seeded in 60 mm plates. Then pre-treated with lower doses (100 nM and 400 nM) of ALA metabolites for 3 h. Cells were then stimulated with or without LPS (100 ng/ml) for next 24 h. Cells were harvested at the end of time point, pelleted down and stored at −80 °C for further use. Cell pellets were lysed in RIPA buffer containing 1x protease inhibitor at 4 °C. Protein estimation was done by Bradford method using BSA as standard. An equal amount of protein was loaded and separated on 8–15% SDS-PAGE and transferred to nitrocellulose membrane. Then membranes were blocked with 5% (w/v) fat-free dry milk in TBST for 1 h at room temperature followed by washing three times with TBST. Membranes were incubated with primary antibodies (0.5–1.0 μg/ml) for 12 h at 4 °C on a shaker incubator with gentle shaking followed by thrice washing with TBST. The membranes were then incubated with respective secondary antibodies conjugated with HRP. Signals were then detected with western lightning plus ECL kit (PerkinElmer) and captured on Kodak Imaging System (KODAK Image station 4000 mm Pro).

Kumar N., Gupta G., Anilkumar K., Fatima N., Karnati R., Reddy G.V., Giri P.V, & Reddanna P. (2016). 15-Lipoxygenase metabolites of α-linolenic acid, [13-(S)-HPOTrE and 13-(S)-HOTrE], mediate anti-inflammatory effects by inactivating NLRP3 inflammasome. Scientific Reports, 6, 31649.

+ Open protocol

+ Expand

Western Blot Analysis of XIAP in Ovarian Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human ovarian cancer cells were lysed with RIPA lysis buffer (BioTeke, Beijing, China) 48 h after transfection. The protein concentration was determined using the bicinchoninic acid protein assay kit (Beyotime, Shanghai, China). Heat-denatured protein samples (20 μg per lane) were separated and then were transferred to a PVDF membrane (Millipore, Bedford, MD, USA) by10% SDS-polyacrylamide gel electrophoresis. The membrane was incubated for 2 h at room temperature or incubated overnight at 4°C with a primary mouse polyclonal antibody against human XIAP (1 : 10 000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or mouse monoclonal antibody against human β-actin (1 : 3000; Abcam, Cambridge, MA, USA) and then was incubated for 1 h at room temperature with a goat anti-mouse secondary antibody (1 : 5000; Abcam, Cambridge, MA, USA). The bound antibody was assessed using enhanced chemiluminescence detection reagents (Pierce, Rockford, IL, USA), and the band intensities were quantified using Kodak Image Station 4000MM Pro (Kodak, Tokyo, Japan) according to the manufacturer's instructions.

Li X., Chen W., Zeng W., Wan C., Duan S, & Jiang S. (2016). microRNA-137 promotes apoptosis in ovarian cancer cells via the regulation of XIAP. British Journal of Cancer, 116(1), 66-76.

+ Open protocol

+ Expand

Extracting and Quantifying Liver and Spleen Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fish liver and spleen samples were manually dissected and immediately lysed in Tissue Extraction Reagent (Thermofisher, Milan, Italy) in the presence of protease inhibitors and phosphatase Inhibitor Cocktails 2 and 3; the lysates were centrifuged at 13,000×g for 30 min at 4 °C. The supernatant was collected and protein concentration was determined by the Bradford method. Extracted proteins were supplemented with 4x sample buffer, heated at 70°C for 10 min and run onto precast SDS-PAGE. Proteins were transferred on Immobilon-P membranes in 25 mM Tris, 192 mM glycine, 20% methanol (v/v), 0.1% SDS. Membranes were incubated overnight with primary antibodies against vitellogenin, FAM20C, beta actin. Primary antibodies (VTG and FAM20C) were used at 1:1000 or 1:5000 (beta-actin) dilution. HRP-conjugated secondary antibodies were used at a 1:3000 dilution. HRP Chemiluminescence signals were detected by Kodak Image Station 4000MMpro (Eastman Kodak, New Haven, CT) and analyzed with the Kodak Image software.

Cozza G., Moro E., Black M., Marin O., Salvi M., Venerando A., Tagliabracci V.S, & Pinna L.A. (2018). The Golgi “casein kinase” Fam20C is a genuine “phosvitin kinase” and phosphorylates polyserine stretches devoid of the canonical consensus. The FEBS journal, 285(24), 4674-4683.

+ Open protocol

+ Expand

Immunoblot Analysis of ER Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were homogenized in hypotonic Tris buffer (10mM Tris, 0.2 mM MgCl₂, PH 7.4) containing protease and phosphatase inhibitor cocktail using a probe sonicator, followed by centrifugation at 12,000 rpm for 15 min at 4° C. The resultant supernatant was used for the immunoblot analysis. Protein concentration was quantified using protein assay reagent (Solarbio, Beijing, China). Equal amounts of protein were resolved by SDS-PAGE gel and electrotransferred to nitrocellular membranes. After blocking, membranes were blotted overnight at 4° C with primary antibodies. Primary antibodies used include anti phospho-eIF2-α (1:1,000, #3398, Cell Signaling Technology, Beverly, MA, USA), anti-eIF2-α (1:1,000, #5324, Cell Signaling Technology), anti-ATF4 (1:1,000, ab85049, Abcam), anti-CHOP (1:1,000, #2895, Cell Signaling Technology), anti-caspase-3 (1:1000, #9662, Cell Signaling Technology), anti-cleaved-caspase-3 (1:1,000, #9664, Cell Signaling Technology), and anti-GAPDH (1:1,000, #5174, Cell Signaling Technology) antibodies. After incubation with HRP-conjugated secondary antibodies, membranes were developed with enhanced chemiluminescence (Thermo Fisher Scientific) using KODAK Image Station 4000MM PRO. Membranes were reblotted with anti-GAPDH for normalization. Densitometric analysis of immunoblots was performed using Image J software.

Ying Y., Xue R., Yang Y., Zhang S.X., Xiao H., Zhu H., Li J., Chen G., Ye Y., Yu M., Liu X, & Zhong Y. (2021). Activation of ATF4 triggers trabecular meshwork cell dysfunction and apoptosis in POAG. Aging (Albany NY), 13(6), 8628-8642.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein extracts (30 μg) from control, Mix. #8, Mix. #10, and Mix. #12 groups, were loaded on 12% SDS polyacrylamide gel. After SDS-PAGE, proteins from gels were electrotransfered in a Semi-Dry Trans-Blot Cell (Bio-Rad- USA) to a nitrocellulose membrane (Hybind ECL, GE healthcare, UK) using transfer buffer (25 mM Tris, 192 mM glycine, and 20% methanol). After an overnight incubation, in blocking buffer (2.5% skim milk, 2.5% glycerol, and 0.05% tween-20 in TBS) at 4ºC, the membrane was washed three times in TTBS (100 mM Tris–HCl, 0.9% NaCl, and 0.05% Tween-20, pH 7.5) for 10 min. It was then incubated for two hours in blocking buffer containing polyclonal antibodies against vimentin (1:5000, Abcam, UK), ferritin heavy chain (1:1000, Zist Fannavaran Sina, Iran), pyruvate dehydrogenase (1:25,000, GeneTex, UK), and monoclonal antibodies against N-myc downstream-regulated gene 1(NDRG1) (1:1000, Abcam, UK) and β-actin as a control protein (1 µg/ml, Sigma-Aldrich, UK). The membrane was then incubated with peroxidase-conjugated anti-mouse and anti-rabbit IgG as secondary antibodies (1:1000, Razi Biotech, Iran). Subsequently, the immunoreactive bands were detected by ECL plus kit (GE healthcare, USA) using Kodak Image Station 4000MM Pro.

Azarian B., Sajedin S.M., Azimi A., Raigani M., Vaziri B, & Davami F. (2017). Proteomics Profiling of Chimeric-Truncated Tissue Plasminogen activator Producing- Chinese Hamster Ovary Cells Cultivated in a Chemically Defined Medium Supplemented with Protein Hydrolysates. Iranian Biomedical Journal, 21(3), 154-166.

+ Open protocol

+ Expand

Western Blot Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysis buffer (Bio-Rad Laboratories, Inc.) was used to prepare the tissue and cells, and protein concentrations were measured by Bradford's method (18 (link)). A total of 50 µg protein was placed in each lane of 12% SDS-PAGE (Bio-Rad Laboratories, Inc.), and then transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Inc.). Fat-free milk powder (5%) was used to block membranes, which was incubated in primary antibodies overnight at 4°C, and then with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, Inc.). A electrochemiluminescent reagent (Pharmacia Corp., Basking Ridge, NJ, USA) was used to detect signals and the Kodak Image Station 4000MM Pro (Kodak, Rochester, NY, USA) was used for analyzing and recording.

Gai J.W., Wahafu W., Song L., Ping H., Wang M., Yang F., Niu Y., Qing W, & Xing N. (2018). Expression of CD74 in bladder cancer and its suppression in association with cancer proliferation, invasion and angiogenesis in HT-1376 cells. Oncology Letters, 15(5), 7631-7638.

+ Open protocol

+ Expand

Protein Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were either treated with inhibitor or DMSO (vehicle) and lysed in RIPA buffer (Solarbio, Beijing, China) supplementing Halt protease/phosphatase inhibitor cocktail (Pierce Biotechnology, Rockford, USA) for 30 min on ice and centrifuged at 12,000g for 15 min at 4 °C. Protein concentrations were determined using BCA assay (Beyotime, Shanghai, China) according to the manufacturer’s instructions. Lysates (150–250 μg) were electrophoretically resolved by SDS-PAGE, transferred to PVDF membrane and probed with the primary antibodies. Proteins were visualized by the use of Super Signal West Pico Chemiluminescent Substrate (Pierce Biotechnology) and detected on a Kodak Image Station 4000MM Pro (New Haven, CT, USA).

Song T., Zhang H., Zhao Q., Hu Z., Wang Z., Song Y, & Zhang Z. (2024). Small molecule inhibitor targeting the Hsp70-Bim protein–protein interaction in estrogen receptor-positive breast cancer overcomes tamoxifen resistance. Breast Cancer Research : BCR, 26, 33.

+ Open protocol

+ Expand

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein concentration of cell lysates was measured by BCA protein assay (Pierce BCA Protein Assay Kit, 23225 Thermo-Fisher Scientifics, Waltham, MA, USA). An amount of 10 μg of proteins was loaded in each lane of 4–12% Bis-Tris NuPAGE gels (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and separated according to the manufacturer’s instructions. After electrophoresis, proteins were transferred to PVDF membranes (IPVH00010, Immobilon -P (PVDF), Merck Millipore, Merck Life Science, Madrid, Spain) for 2 h. Membranes were blocked in 5%milk for 1 h and then incubated in the appropriate primary antibodies (diluted in Superblock solution, Thermo-Fisher Scientifics, Waltham, MA, USA) overnight. Bands were visualized after incubation with the respective horseradish peroxidase-linked secondary antibodies using Enhanced Chemiluminescence (ECL) in the digital imaging system Kodak Image Station 4000MM PRO. Then, bands were quantified using the ImageJ software (v. 1.52, NIH, https://imagej.nih.gov/ij/index.html accessed on 21 May 2022).

Ruz C., Alcantud J.L., Vives F., Arrebola F., Hardy J., Lewis P.A., Manzoni C, & Duran R. (2022). Seventy-Two-Hour LRRK2 Kinase Activity Inhibition Increases Lysosomal GBA Expression in H4, a Human Neuroglioma Cell Line. International Journal of Molecular Sciences, 23(13), 6935.

+ Open protocol

+ Expand

Western Blot Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described [24 (link)] using primary antibodies indicated in Table 1. HRP-conjugated secondary antibodies (1:2000) were from Cell Signalling Technology. Immunoreactive bands were revealed by enhanced chemiluminescence (SuperSignal™ West Pico Chemiluminescent Substrate kits, Thermo Scientific) and visualized by the KODAK Image Station 4000MM PRO. Band intensities were quantified by scanning densitometry (Gel-Doc2000, Bio-Rad) and analyzed with Quantity One™ (Bio-Rad).

Zhu H., Zhang W., Zhao Y., Shu X., Wang W., Wang D., Yang Y., He Z., Wang X, & Ying Y. (2018). GSK3β-mediated tau hyperphosphorylation triggers diabetic retinal neurodegeneration by disrupting synaptic and mitochondrial functions. Molecular Neurodegeneration, 13, 62.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!