Dmp900

Manufactured by R&D Systems

Sourced in United States

The DMP900 is a digital pressure meter designed for measuring pressure in a variety of applications. It features a high-resolution display and provides accurate and reliable pressure readings. The device is equipped with various connectivity options for data transfer and integration with other systems.

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Lab products found in correlation

20 protocols using dmp900

Biomarker Assays and Doxycycline Measurements

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Doxycycline and biomarker assays are performed on specimens frozen in cryovials labeled with numbers that cannot be linked to PID/Letcode except through the DCC and to individuals in the clinical sites only. We use an ultra high performance liquid chromatography (UPLC) tandem mass spectrometry (MS/MS) method at the University of Maryland School of Pharmacy to measure serum doxycycline levels.
Analysis of hs-CRP will be performed using an immunoturbidimetric latex agglutination method (K-Assay [KAI-060], Kamiya Biomedical Co., Seattle, WA). Serum cotinine will be measured with an ELISA (Calbiotech, Spring Valley, CA). Plasma MMP-9 concentrations will be measured by an ELISA, two-site sandwich method that is commercially available (R & D Systems, Quantikine, DMP900). Interferon-gamma will be measured by a sandwich-type ELISA also (R&D Systems, Quantikine, DMP900).

Baxter B.T., Matsumura J., Curci J., McBride R., Blackwelder W.C., Liu X., Larson L, & Terrin M.L. (2016). Non-Invasive Treatment of Abdominal Aortic Aneurysm Clinical Trial (N-TA3CT): Design of a Phase IIb, Placebo-Controlled, Double-Blind, Randomized Clinical Trial of Doxycycline for the Reduction of Growth of Small Abdominal Aortic Aneurysm. Contemporary clinical trials, 48, 91-98.

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Quantifying MMP-2 and MMP-9 in OSCC Cell Lines

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According to a previous study (19 (link)), the OSCC Cal-27 and SCC-9 cell culture supernatant was collected after treatment. Using ELISA kits (cat. nos. MMP200 and DMP900; R&D Systems, Inc.) the concentrations of MMP-2 and MMP-9 were measured, according to the manufacturer's protocol.

Kang Y., Zhang Y, & Sun Y. (2021). MicroRNA-198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4. International Journal of Oncology, 59(1), 39.

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Comprehensive Cytokine Profiling in Viral Infection

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Cytokine analysis was performed on plasma, CSF, and brain samples using Biolegend LEGENDplex 13-plex kit (Cat. No. 740389). Lethal brain samples were diluted 1:2 in assay buffer, plasma was run at 1:4 dilution, and convalescent brain or uninfected animals as well as all cerebral spinal fluid were run undiluted. Samples were prepared following LEGENDplex protocol for v-bottom kit. Samples were then run on FACSAria flow cytometer immediately following staining. Results were then analyzed using LEGENDplex Data Analysis Software. Analytes measured were interleukin (IL)-6, IL-10, IP-10, IL-β, IL-12p40, IL-17A, IFN-β, IL-23, TNF-α, IFN-γ, GM-CSF, IL-8 and MCP-1. An ELISA assay was used to measure MMP9 levels in plasma (R&D Systems; DMP900).

Albe J.R., Ma H., Gilliland T.H., McMillen C.M., Gardner C.L., Boyles D.A., Cottle E.L., Dunn M.D., Lundy J.D., O’Malley K.J., Salama N., Walters A.W., Pandrea I., Teichert T., Klimstra W.B., Reed D.S, & Hartman A.L. (2021). Physiological and immunological changes in the brain associated with lethal eastern equine encephalitis virus in macaques. PLoS Pathogens, 17(2), e1009308.

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Quantification of MMP-9 and TIMP

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Human and murine MMP-9 and TIMP concentration were quantified using an established ELISA assay (#DMP900; human MMP-9, #MMPT90; mouse MMP-9, #DTM 100; human TIMP, #MTM100; mouse TIMP; R&D Systems) following the manufacturer’s protocol. MMP-9 and TIMP concentrations (in pg) were adjusted for the amount of protein (in mg; Catalog #5000112; BioRad) that was present in the sample (reported as pg/mg).

Kong M.Y., Whitley R.J., Peng N., Oster R., Schoeb T.R., Sullender W., Ambalavanan N., Clancy J.P., Gaggar A, & Blalock J.E. (2015). Matrix Metalloproteinase-9 Mediates RSV Infection in Vitro and in Vivo. Viruses, 7(8), 4230-4253.

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Urinary MMP9 and MMP3 Quantification

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Urinary MMP9 and MMP3 were measured, using a commercially available ELISA kits provided by R&D Systems, Inc.614 McKinley, United States of America with catalog number DMP900 and from Cusabio with calaloge number of CSB-E04677h, according to manufacturer instructions.
Two microplates of 96 wells each has been pre-coated with monoclonal antibodies specific for MMP-9 and MMP3. Standards and urine samples were pipetted into the wells of each microplate. After washing away unbounded substances, an enzyme-polyclonal antibody specific for each MMp-9 and MMP3 was added to the wells of each plate. Following washing to remove unbound antibody- enzyme reagent, a substrate solution was added to the wells of each plate and a color was developed in proportion to the amount of MMP-9and MMP3 bounded in the initial step. The intensity of the color was measured at specific wave length. Standard curves were plotted using standard concentrations on X axis and their optical densities on Y axis.
The concentrations of MMP-9 and MMP3 in urine samples were determined by comparing the optical densities (OD) of the samples to the standard curves.

El-Sharkawi F., El Sabah M., Hassan Z, & Khaled H. (2014). The biochemical value of urinary metalloproteinases 3 and 9 in diagnosis and prognosis of bladder cancer in Egypt. Journal of Biomedical Science, 21(1), 72.

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Serum MMP3 and MMP9 Quantification

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Serum MMP3 levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (DMP300; R&D Systems, Minneapolis, MN, United States). The detection range of this kit is 0.2–10 ng/ml, the sensitivity is 0.045 ng/mL, and the intra- and interdetection variability ranges are 5.7∼6.4% and 7.0∼8.6%, respectively. Serum MMP9 levels were measured using an ELISA kit (DMP900; R&D Systems, Minneapolis, MN, United States). The detection range of the kit is 0.3–20 ng/mL, the sensitivity is 0.156 ng/mL, and the intra- and interdetection variability ranges are 1.9∼2.9% and 6.9∼7.9%, respectively. Biological replicates were analyzed on the same plate according to the manufacturer’s instructions.

Liu C.Z., Guo D.S., Ma J.J., Dong L.R., Chang Q.Q., Yang H.Q., Liang K.K., Li X.H., Yang D.W., Fan Y.Y., Gu Q., Chen S.Y, & Li D.S. (2022). Correlation of matrix metalloproteinase 3 and matrix metalloproteinase 9 levels with non-motor symptoms in patients with Parkinson’s disease. Frontiers in Aging Neuroscience, 14, 889257.

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Zey Modulates MMP2/9 Expression

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Cells were inoculated in 6-well plates (2.5×10⁴ cell/well) and cultured in an incubator for 24 h. Cells were treated with different concentrations of Zey for 48 h at 10 (Zey1), 20 (Zey2) and 40 (Zey3) µmol/l. Untreated cells served as a control. Subsequently, cells were digested with 0.25% EDTA-trypsin (Beijing Solarbio Science & Technology Co., Ltd.) and centrifuged at 800 × g for 5 min at 4°C. Following centrifugation, cells were resuspended in F-12K medium. The expression of MMP2/9 was detected using ELISA test kits (cat. nos. MMP200 and DMP900, respectively; R&D Systems, Inc., Minneapolis, MN, USA). The standard curve was plotted with standard samples. The assay diluent (50 µl) was added to each well. In total, 50 µl sample in each group was added into each well. The plates was covered with plate sealer and maintained for 2 h at room temperature. Following washing, the conjugate reagent was added into each well and the plate was placed on a shaker at room temperature for 2 h. The substrate solution was added into each well and maintained for 30 min at room temperature. Subsequently, the stop solution was used to terminate the reaction. Finally, the absorbance at 450 nm was read using a FilterMax F3/F5 microplate reader.

Zeng S., Zhu B., Zeng J., Wu W, & Jiang C. (2018). Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/β-catenin pathway. Molecular Medicine Reports, 18(6), 5572-5578.

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Quantifying TGF-β, MMP-2, and MMP-9

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The concentrations of TGF-β (R&D System, DB250), MMP-2 (R&D System, DMP2F0), and MMP-9 (R&D System, DMP900) in the culture supernatant were measured using an enzyme linked immunosorbent assay kit following the manufacture’s protocol.

Kim J.Y., Choi Y.J, & Kim H.J. (2021). Determining the effect of ellagic acid on the proliferation and migration of pancreatic cancer cell lines. Translational Cancer Research, 10(1), 424-433.

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Murine Osteoclast Protein Analysis

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Murine osteoclast-like cells were PBS washed,
homogenized and lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4; 150
mM NaCl; 1% NP-40; 0.5% Na-deoxycholate; 0.1% SDS; 2 mM EDTA; 10 mM
NaF) containing protease inhibitors (Pierce, according to manufacturer’s
specifications) with a 1 mL syringe and a 23-gauge needle. After centrifugation
at 15 000 rpm at 4 °C for 10 min, supernatants were frozen
at −80 °C. Protein concentrations were assayed using a
Pierce BCA Kit, and 30 μg of protein per lane was subjected
on 10% SDS gel. Proteins were electroblotted onto PVDF, and blots
were probed overnight at 4 °C (anti-NFATc1 (1:500; 7A6: sc-7294
mouse monoclonal, Santa Cruz) or anti-Lamin B1 (1:10 000; ab133741
rabbit monoclonal, Abcam). Secondary antibodies were HRP-conjugated
sheep antimouse (1:5000 for Nfatc1; #NA93IV; GE Healthcare, Piscataway,
NJ) or HRP-conjugated goat antirabbit (1:5000 for Lamin B1; # P0448;
Dako, Carpinteria, CA). MMP9 secretion in the human osteoclast supernatant
was determined by a MMP9 ELISA (R&D, DMP900) according to the
manufacturer’s instructions. The supernatant (50 μL/well)
was collected, frozen at −20 °C, and 100-fold diluted
for the assay. Absorbance was measured at 440 nm.

Meier J.C., Tallant C., Fedorov O., Witwicka H., Hwang S.Y., van Stiphout R.G., Lambert J.P., Rogers C., Yapp C., Gerstenberger B.S., Fedele V., Savitsky P., Heidenreich D., Daniels D.L., Owen D.R., Fish P.V., Igoe N.M., Bayle E.D., Haendler B., Oppermann U.C., Buffa F., Brennan P.E., Müller S., Gingras A.C., Odgren P.R., Birnbaum M.J, & Knapp S. (2017). Selective Targeting of Bromodomains of the Bromodomain-PHD Fingers Family Impairs Osteoclast Differentiation. ACS Chemical Biology, 12(10), 2619-2630.

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Measuring Angiogenic Biomarkers in Serum

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Peripheral blood samples were collected from all participants at recruitment as part of standard care and serum was processed by the Townsville University Hospital Pathology Department (Pathology Queensland) prior to storing at − 80 °C for later analysis. Serum angpt-1, matrix-metalloproteinase-9 (MMP-9) and Tie-2 concentrations were measured using commercially available ELISAs according to manufacturer’s directions (DANG 10, DMP900 and DTE200, respectively, R&D Systems, Minneapolis, USA). Serum angiopoietin-2 (angpt-2), and vascular endothelial growth factor (VEGF)-A, -C and -D concentrations were measured using the MilliPLEX MAP Human Angiogenesis/Growth Factor Magnetic Bead Panel—Cancer Multiplex Assay kit (HAGP1MAG-12 K, Merck, Australia), using the MagPIX platform, and analyses were conducted by a researcher blinded to patient diagnosis. Samples in which the assessed biomarker fell outside of the detectable limits of the test were excluded from analysis (detailed in Supplement 1). Biomarker analysis was conducted independently of patient care and had no potential to influence clinical outcome.

Moxon J.V., Kraeuter A.K., Phie J., Juliano S., Anderson G., Standley G., Sealey C., White R.P, & Golledge J. (2022). Serum angiopoietin-1 concentration does not distinguish patients with ischaemic stroke from those presenting to hospital with ischaemic stroke mimics. BMC Cardiovascular Disorders, 22, 462.

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