Aspartate aminotransferase assay kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Aspartate Aminotransferase Assay Kit is a laboratory tool used to measure the activity of the enzyme aspartate aminotransferase (AST) in biological samples. AST is an enzyme involved in amino acid metabolism and is commonly used as a biomarker for various medical conditions.

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Aspartate aminotransferase (64 citations) Aspartate aminotransferase (AST) assay kit (9 citations)

32 protocols using aspartate aminotransferase assay kit

Enzymatic Activity Profiling in Aquatic Organisms

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In this study, the enzymatic activities of pepsin, lipase, and α-amylase in the intestine and stomach were detected using a Pepsin Assay Kit, a Lipase Assay Kit, and an α-Amylase Assay Kit (Nanjing Jiancheng, Bioengineering Institute, China). The enzymatic activities of ACP, AKP, LDH, SOD, CAT, GPT, and GOT in the gill, brain, intestine, stomach, kidney, liver, and plasma were detected using an Acid Phosphatase Assay Kit, an Alkaline Phosphatase Assay Kit, a Lactate Dehydrogenase Assay Kit, a Superoxide Dismutase Assay Kit, a Catalase (CAT) Assay Kit, an Alanine Aminotransferase Assay Kit, and an Aspartate Aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, China). Total protein was determined with a Total Protein Assay Kit (Nanjing Jiancheng Bioengineering Institute, China). The operation steps were carried out according to the operation guide of the reagent kit, and the operation guide of the corresponding reagent kit can be searched for on the website (http://www.njjcbio.com/, accessed on 8 June 2023). In addition, the calculation formulas for these enzyme activities are detailed in the Supplemental Material.

Zhou J., Li Q., Huang Z., Zhang L., Mou C., Zhao Z., Zhao H., Du J., Yang X., Liang X, & Duan Y. (2023). Study on the Adaptive Regulation of Light on the Stress Response of Mandarin Fish (Siniperca chuatsi) with Re-Feeding after Starvation. Animals : an Open Access Journal from MDPI, 13(16), 2610.

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Determination of Liver Enzymes

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After blood collection, serum was separated by centrifugation at 3,200 × g for 20 min at room temperature. The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum from rats were determined using commercially available diagnostic kits (Alanine aminotransferase assay kit; cat no. C009-2; Aspartate aminotransferase assay kit; cat no. C010-2; Nanjing Jiancheng Bio Co., Ltd., Nanjing, China) according to the manufacturer's instructions.

Yang C., Li L., Ma Z., Zhong Y., Pang W., Xiong M., Fang S, & Li Y. (2017). Hepatoprotective effect of methyl ferulic acid against carbon tetrachloride-induced acute liver injury in rats. Experimental and Therapeutic Medicine, 15(3), 2228-2238.

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In Vivo Biocompatibility of TMNPs

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H&E analysis was used to investigate the biocompatibility of TMNPs in vivo. Healthy BALB/c mice were randomly assigned to each group (n = 5). All the mice treated with different preparations (PTX@TF, MMST/LTSLs, TF/LTSLs, and TMNPs) were injected into tail vein (saline was used for the control group) seven times every 3 days. Finally, the mice were euthanized to isolate the major tissues for H&E analysis. The blood was sampled and centrifuged at 4000 RPM for 10 min to separate the serum. The levels of ALT and AST in the serum were measured using an Alanine aminotransferase Assay Kit (Nanjing Jiancheng, Cat: C009-2-1) and an Aspartate aminotransferase Assay Kit (Nanjing Jiancheng, Cat: C010-2-1) according to the manufacturer’s instructions.

Zhang K., Li J., Xin X., Du X., Zhao D., Qin C., Han X., Huo M., Yang L, & Yin L. (2021). Dual Targeting of Cancer Cells and MMPs with Self-Assembly Hybrid Nanoparticles for Combination Therapy in Combating Cancer. Pharmaceutics, 13(12), 1990.

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Evaluating Organ Damage in Ischemia-Reperfusion

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Lung,⁵² (link) liver,⁵³ (link) and kidney⁵⁴ (link) histopathological damage scores were assessed 6 h after reperfusion as described previously. The lung wet/dry (W/D) weight ratio was measured as previously described.⁵⁵ (link) An alanine aminotransferase (ALT) assay kit (C009-2-1, Nanjing Jiancheng Bioengineering Institute) and aspartate aminotransferase assay kit (C010-2-1, Nanjing Jiancheng Bioengineering Institute) were used to detect ALT and AST levels in plasma to reflect liver damage. A creatinine (Cr) assay kit (C011-2-1, Nanjing Jiancheng Bioengineering Institute) was used to detect Cr levels in plasma to reflect kidney damage. The specific steps of all kits are shown in the kit instructions.

Deng F., Hu J.J., Lin Z.B., Sun Q.S., Min Y., Zhao B.C., Huang Z.B., Zhang W.J., Huang W.K., Liu W.F., Li C, & Liu K.X. (2023). Gut microbe-derived milnacipran enhances tolerance to gut ischemia/reperfusion injury. Cell Reports Medicine, 4(3), 100979.

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Biomarker Analysis of Serum Samples

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The total cholesterol (TC), total triglyceride (TG), glutamic oxaloacetic transaminase (GOT/AST), glutamic pyruvic transaminase (GPT/ALT) and LPS in serums were detected by the corresponding kits. TC and TG in serum were determined by single reagent GPO-PAP method using total cholesterol assay kit (A111-1-1, Nanjing Jiancheng, China) and triglyceride assay kit (A110-1-1, Nanjing Jiancheng, China). GPT and GOT in serum were detected by Reiss method and microplate method, GTA Kit (C009-2-1, Nanjing Jiancheng, China) and aspartate aminotransferase assay kit (C010-2-1, Nanjing Jiancheng, China). The content of LPS in serum was determined by using a lipopolysaccharide assay kit (H255, Nanjing Jiancheng, China).

Wang Y., Zhang Y., Yang J., Li H., Wang J, & Geng W. (2021). Lactobacillus plantarum MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier. Foods, 10(12), 3126.

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Inflammatory Markers in Mouse LPS Model

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Plasma concentration of LPS was determined using a Mouse Lipopolysaccharides ELISA Kit (CUSABIO Biotech Co., Ltd., China). Alanine aminotransferase assay kit, Aspartate aminotransferase assay kit, Superoxide Dismutase (SOD) assay kit (WST-1 method), Nitric Oxide Synthase (NOS) typed assay kit (Colormetric method) and Triglyceride assay kit were from Nanjing Jiancheng Bioengineering Institute, China. Samples of 8–12 mice in each group were randomly selected for the assays.

Xiang J.Y., Chi Y.Y., Han J.X., Shi X., Cai Y., Xiang H, & Xie Q. (2022). Intestinal Microbiota Contributes to the Improvement of Alcoholic Hepatitis in Mice Treated With Schisandra chinensis Extract. Frontiers in Nutrition, 9, 822429.

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Hepatic Function Assessment in Mice

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To assess the hepatic function of the mice, the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ‐glutamyl transferase (GGT), alkaline phosphatase (AKP), total bilirubin (TBil), and direct bilirubin (DBil) levels were measured as per the manufacturer's instruction. Alanine aminotransferase assay kit (C009‐2‐1; Jiancheng, Nanjing, China), aspartate aminotransferase assay kit (C010‐2‐1; Jiancheng), γ‐Glutamyl transferase assay kit (C017‐2‐1; Jiancheng), alkaline phosphatase assay kit (A059‐2; Jiancheng), direct bilirubin kit (C019‐2‐1; Jiancheng) and total bilirubin kit (C019‐1; Jiancheng) were used here.

Wu B., Tian X., Wang W., Zhu J., Lu Y., Du J, & Xiao Y. (2022). Upregulation of cadherin‐11 contributes to cholestatic liver fibrosis. Pediatric Investigation, 6(2), 100-110.

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Histological and Biochemical Liver Assessment

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The left lobe of mouse liver was placed in 10% formaldehyde, dehydrated and then embedded in paraffin blocks. It was sectioned to 4 µm thickness for staining using Masson's Trichrome Stain Kit and Picrosirius Red from Solarbio Technology Co., Ltd. Moreover, we also used the paraffin section for dewaxing to water and antigen repair. After the liver slices were incubated with primary antibody, we used UltraSensitive™ SP (Mouse/Rabbit) IHC Kit from Maixin Biotech. Co., Ltd for staining. We used the TUNEL staining method by using the Colorimetric TUNEL Apoptosis Assay Kit (Beyotime Biotechnology) to observe the apoptosis of liver tissue. We randomly selected the field of view to take pictures using upright transmission fluorescence 162 microscope (Olympus) and analysed the photos using Image‐Pro Plus Version 6.0 (Media 163 Cybernetics, Inc American). Apart from these, we used the alanine aminotransferase assay kit and aspartate aminotransferase assay kit from Nanjing Jiancheng Bioengineering Institute (Nanjing, China) for ALT and AST content determination, respectively.

Li X., Shao S., Li H., Bi Z., Zhang S., Wei Y., Bai J., Zhang R., Ma X., Ma B., Zhang L., Xie C., Ning W., Zhou H, & Yang C. (2020). Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice. Journal of Cellular and Molecular Medicine, 24(15), 8623-8635.

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Mouse Serum Biomarker Quantification

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Mouse blood samples were collected by orbital venous plexus bleeding at the indicated time, mouse blood samples were left to clot, centrifuged (8000xg, 20 min) and then the serum was collected and stored at −80 °C. Serum levels of mouse ALT and AST were measured by alanine aminotransferase assay kit (Cat #: C009-2-1, Nanjing Jiancheng Bioengineering Institute) and aspartate aminotransferase assay kit (Cat #: C010-2-1, Nanjing Jiancheng Bioengineering Institute), and human cytokines were detected by ELISA using the BD™ CBA Human Th1/Th2 Cytokine Kit (Cat #: 550749, BD Biosciences).

Liao Q., Liu Z., Zhu C., He H., Feng M., Jiang L., Ding X., Sun R., Zhang X, & Xu J. (2022). Rapid generation of a mouse model for evaluating on-target normal tissue toxicity of human CAR-T cells using replication-defective recombinant adenovirus. Journal of Advanced Research, 47, 163-171.

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Antioxidant and Hepatic Enzyme Assays

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Powdered Act V and Act D (purity 98%, obtained from Dr. Xie at Shandong University) and dimethylsulfoxide (DMSO) were prepared for a 1 μmol/L and 10 μmol/L stock solution, diluted with the cell culture medium in the samples. MTT was acquired from Sigma-Aldrich Corp. (St. Louis, MO, USA). Reactive Oxygen Species Assay Kit (S0033S), Lipid Peroxidation MDA Assay Kit (S0131S), Total Glutathione Peroxidase Assay Kit (S0058), Total Superoxide Dismutase Assay Kit with NBT (nitroblue tetrazolium) (S0109), and Hematoxylin and Eosin Staining Kit (C0105) were obtained from the Beyotime Institute of Biotechnology (Shanghai, China). Alanine aminotransferase Assay Kit (C009-2-1), Aspartate aminotransferase Assay Kit (C010-2-1), and Creatinine (Cr) Assay Kit (sarcosine oxidase) (C011-2-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China).

Jia F.J., Han Z., Ma J.H., Jiang S.Q., Zhao X.M., Ruan H., Xie W.D, & Li X. (2020). Involvement of Reactive Oxygen Species in the Hepatorenal Toxicity of Actinomycin V In Vitro and In Vivo. Marine Drugs, 18(8), 428.

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