Avance 300 mhz spectrometer

Manufactured by Bruker

Sourced in United States, Germany, Switzerland, France

The Avance 300 MHz spectrometer is a nuclear magnetic resonance (NMR) instrument designed for analytical applications. It operates at a radiofrequency of 300 MHz and is capable of performing various NMR spectroscopy experiments to analyze the structure and properties of chemical compounds.

Automatically generated - may contain errors

Lab products found in correlation

Initiator alstra, by Biotage (2 mentions) Q tof mass spectrometer, by Bruker (2 mentions) 515 hplc pump, by Wyatt Technology (2 mentions) Uv 2600, by Shimadzu (2 mentions) Ftir 8400, by Shimadzu (2 mentions) Nicolet nexus 670 spectrophotometer, by Thermo Fisher Scientific (1 mentions) 60f254 silica gel plate, by Merck Group (1 mentions) Fischer johns block, by Thermo Fisher Scientific (1 mentions) Fv1000 confocal fluorescence microscope, by Olympus (1 mentions) 1290lc 6540 accurate mass q tof, by Agilent Technologies (1 mentions) 1260 series hplc system, by Agilent Technologies (1 mentions) 2 ethyl 2 oxazoline etox, by Merck Group (1 mentions) Acquity uplc instrument, by Waters Corporation (1 mentions) Series 200 hplc, by PerkinElmer (1 mentions) Asap 2020 surface area and porosity analyzer, by Micromeritics (1 mentions) Nova nanosem 450, by Thermo Fisher Scientific (1 mentions) Nanostarinstrument, by Bruker (1 mentions) D2 phaser, by Bruker (1 mentions) V 730 uv vis spectrophotometer, by Jasco (1 mentions) Nano zs90 instrument, by Malvern Panalytical (1 mentions)

52 protocols using avance 300 mhz spectrometer

Characterization of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the chemicals were obtained from Sigma-Aldrich chemical company (USA), Lancaster (USA) and S.D. Fine chem. Limited (Mumbai). All the glassware is of borosilicate grade. Melting points were determined in open capillaries and are uncorrected. The purity of the compounds was ascertained by thin-layer chromatography (TLC) on silica gel-G plate.
Fourier transform infrared (IR) spectra were taken in KBr on a Thermo Nicolet Nexus 670 spectrophotometer. 1 H nuclear magnetic resonance (NMR) spectra were recorded on AVANCE 300 MHz spectrophotometer in CDCl 3 with tetramethylsilane (TMS) as internal standard. 13 C NMR spectra were recorded on BRUKER AVANCE 300MHz spectrometer in CDCl 3 with TMS as an internal standard. The chemical shift values are in δppm. Mass spectra were recorded on Polaris Q apparatus (Thermo Electron) and the fragmentations were obtained by electronic impact (EI). The data are given as mass to charge ratio (m/z) and nominal masses were used for the calculation of molecular weights of the prepared products.

PERKA H., SATYAVATI D., GADE D.R., BOYA V., & VVS R.P. (2020). DESIGN SYNTHESIS OF NOVEL ACRIDINE TAGGED PYRAZOLE DERIVATIVES AS AURORA KINASE INHIBITORS. Asian Journal of Pharmaceutical and Clinical Research.

+ Open protocol

+ Expand

Physicochemical Characterization of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

All commercial reactants and solvents with the highest purity were purchased from either Sigma-Aldrich (St. Louis, MS, USA) or Alfa Aesar (Karlsruhe, Germany) and used without further purification. The melting points were determined on a Fischer-Johns block (Fisher Scientific, Schwerte, Germany) and are uncorrected. Elemental analyses were determined by an AMZ-CHX elemental analyzer (PG, Gdańsk, Poland) and are within ±0.4% of the theoretical values. ¹H-NMR spectra were recorded on an Avance 300 MHz) spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany). Analytical thin layer chromatography was performed with 60F₂₅₄ silica gel plates (Merck, Darmstadt, Germany) and visualized by UV irradiation (254 nm). The physicochemical characterization of compounds 7 and 18 was presented in [43 (link),44 (link)]. The structure of 4 was presented in our previous publication [45 (link)], however no physicochemical characterization data was reported.

Paneth A., Węglińska L., Bekier A., Stefaniszyn E., Wujec M., Trotsko N, & Dzitko K. (2019). Systematic Identification of Thiosemicarbazides for Inhibition of Toxoplasma gondii Growth In Vitro. Molecules, 24(3), 614.

+ Open protocol

+ Expand

Solid-State NMR Spectroscopy of Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solid-state nuclear magnetic resonance (SSNMR) spectra were acquired on a Bruker Avance 300 MHz spectrometer, equipped with a 7.05 T wide bore magnet. The experiments were performed at room temperature using a standard 4-mm magic angle spinning (MAS) probe with MAS rates of 10 kHz. A rotor-synchronized Hahn-echo sequence was employed with a π/2 pulse of 2 μs and a recycle delay of 5 or 10 s. Chemical shifts are referenced relative to ultrapure Milli-Q water (shift: + 4.7 ppm).

Musumeci V., Goracci G., Sanz Camacho P., Dolado J.S, & Aymonier C. (2021). Correlation between the Dynamics of Nanoconfined Water and the Local Chemical Environment in Calcium Silicate Hydrate Nanominerals. Chemistry (Weinheim an der Bergstrasse, Germany), 27(44).

+ Open protocol

+ Expand

Synthesis and Characterization of Fluorescent Probe for HOCl Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

All solvents and chemicals were purchased from commercial suppliers and were used without further purification unless otherwise stated. Column chromatography was conducted on silica gel (200–300 mesh) and thin layer chromatography (TCL) was performed using silica gel 60 F254 (Qingdao Ocean Chemicals, Qingdao, China). ¹H NMR and ¹³C NMR spectra were recorded on a Bruker Avance 300 MHz spectrometer. High resolution mass spectra (HRMS) were recorded on an Agilent 1290LC-6540 Accurate Mass Q-TOF by using electrospray ionization (ESI). Fluorescent spectra were measured by using TU-1901 (Beijing Purkinje General Instrument Co., Ltd.) and F-280 (Tianjin Gangdong Technology Co., Ltd.). The cytotoxicity assay measures the absorption wavelength at 490 nm by means of an enzyme labeling device (Synergy HT). Fluorescence imaging of HOCl in live HeLa cells was measured under an Olympus FV1000 confocal fluorescence microscope.

Jiao C., Liu Y., Lu W., Zhang P., Ma X, & Wang Y. (2019). A simple sensor based on 1,8-naphthalimide with large Stokes shift for detection of hypochlorous acid in living cells. RSC Advances, 9(54), 31196-31201.

+ Open protocol

+ Expand

Characterization of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sigma, Riedel-de Haën, and Merck, what provide the all reagents used. The 1 H and 13 C NMR spectra were determined on a Bruker Avance 300 MHz Spectrometer (Germany) at 300 MHz and 75 MHz, respectively, in CDCl 3 using tetramethylsilane as the internal calibration standard. IR spectra were saved with a Vertex 80v FT-IR (Germany). Melting point measurements were made by Electrothermal 9100 Melting Point Apparatus (China). Kinetic measurements of all compounds were obtained with a GBC Cintra 20 model Ultraviolet-Visible (UV-VIS) Spectrophotometer (Australia).

Yakan H., Ozturk S., Uyar Tolgay E., Yenigun S., Marah S., Doruk T., Ozen T, & Kutuk H. (2023). Kinetic Studies, Antioxidant Activities, Enzyme Inhibition Properties and Molecular Docking of 1,3-Dihydro-1,3-Dioxoisoindole Derivatives. Acta chimica Slovenica, 70(1).

+ Open protocol

+ Expand

NMR Characterization of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

¹H and ¹³C NMR spectra were recorded at 298 K on an Avance 300 MHz spectrometer (300 and 75 MHz, respectively; Bruker BioSpin). ¹H-¹H correlation spectroscopy (COSY) and ¹H-¹3C heteronuclear single quantum coherence (HSQC) were used to confirm ¹H and ¹³C assignments. Signals are reported as chemical shift (δ in ppm) relative to (CD₃)₂SO (¹H NMR: δ = 2.50 ppm; 13 C NMR: δ = 39.52 ppm). Coupling constants (J) are reported in Hz and can be found in the Supplementary Information.

Shiels R.G., Vidimce J., Pearson A.G., Matthews B., Wagner K.H., Battle A.R., Sakellaris H, & Bulmer A.C. (2019). Unprecedented Microbial Conversion of Biliverdin into Bilirubin-10-sulfonate. Scientific Reports, 9, 2988.

+ Open protocol

+ Expand

Synthesis and Characterization of Functional Polymers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Materials. Acetonitrile and triethylamine (Aldrich) were dried in a solvent purification system (J. C. Meyer). 2-Ethyl-2-oxazoline (EtOx; Aldrich) was distilled over barium oxide and stored under argon. Methyl tosylate (MeOTs) was distilled and stored under argon. Piperazine was purified by sublimation and stored under argon. (1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethyl (4-nitrophenyl) carbonate (BCN-PNP) was purchased from SynAffix and stored at -20°C. All other chemicals were purchased from Sigma-Aldrich or Acros Organics and used as received.
Instrumentation. Polymerizations were performed in a capped vial in a microwave reactor (Biotage Initiator Sixty) equipped with an IR temperature sensor. 1 H NMR spectra were recorded in CDCl 3 on a Bruker Avance 300 MHz spectrometer. Size-exclusion chromatography (SEC) was performed on a Agilent 1260-series HPLC system equipped with a 1260 online degasser, a 1260 ISO-pump, a 1260 automatic liquid sampler (ALS), a thermostatted column compartment (TCC) at 50°C equipped with two PLgel 5 µm mixed-D columns and a precolumn in series, a 1260 diode array detector (DAD) and a 1260 refractive index detector (RID) using N,Ndimethylacetamide containing 50 mM of LiCl (flow rate of 0.59 mL min -1 ) as solvent. Molar masses were calculated against polymethylmethacrylate standards.

Glassner M., Palmieri L., Monnery B.D., Verbrugghen T., Deleye S., Stroobants S., Staelens S., Wyffels L, & Hoogenboom R. (2017). The Label Matters: μPET Imaging of the Biodistribution of Low Molar Mass ⁸⁹Zr and ¹⁸F-Labeled Poly(2-ethyl-2-oxazoline). Biomacromolecules, 18(1).

+ Open protocol

+ Expand

NMR Spectroscopy of PGT-Hormone Complexation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nuclear magnetic resonance spectra were obtained using an AVANCE 300 MHz spectrometer (Bruker). The samples (PGT and the IC in a 1:1 molar ratio) were dissolved in deuterated chloroform (CDCl₃) at 25 °C. A chemical shift (δ) of 7.26 ppm for CDCl₃ was used as internal reference.
The variation of chemical shift (Δδ) of the protons in the PGT due to the inclusion of the hormone into the cavity of the CD was calculated applying the following equation:

Δ δ = δ_{I C} - δ_{F r e e}

where δ_IC is the proton shift of the PGT in the IC and δ_Free is the proton shift of the PGT when it is free i.e. not included.

Velázquez N.S., Turino L.N., Luna J.A, & Mengatto L.N. (2019). Progesterone loaded thermosensitive hydrogel for vaginal application: Formulation and in vitro comparison with commercial product. Saudi Pharmaceutical Journal : SPJ, 27(8), 1096-1106.

+ Open protocol

+ Expand

Characterization of Novel Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

General methods. All purchased chemicals were of the highest purity commercially available and used without further purification. Analytical grade solvents were used as received. Unless otherwise noted, all reactions were carried out under a nitrogen atmosphere. They were monitored by TLC on silica gel Alugram® Xtra SIL G/UV254. Column chromatography was performed on Machery-Nagel silica gel or neutral alumina.
NMR, mass and infrared spectra were obtained in the relevant 'Services communs de l'Institut de Chimie de Toulouse, Université de Toulouse III Paul-Sabatier'. 1 H-and 13 C-NMR spectra were recorded on a Bruker Avance 300 MHz spectrometer. Attributions of the signals were made using 2D NMR data (COSY, HSQC and HMBC). Protons and carbon atoms were numbered according to Fig. S25 †. Signals are described as follow: s, singlet; d, doublet; t, triplet; m, multiplet. App = Apparent; * = The multiplicity of the signal is more complex as it is part of an AAXX system. All spectra are given in Fig. were not possible with this apparatus. Fluorescence microscopy was performed with a Leitz Laborlux D fluorescence microscope equipped with an Andor Luca camera.

Poirot A., Vanucci-Bacqué C., Delavaux-Nicot B., Leygue N., Saffon-Merceron N., Alary F., Bedos-Belval F., Benoist E, & Fery-Forgues S. (2021). Phenyl-pyta-tricarbonylrhenium(I) complexes: combining a simplified structure and steric hindrance to modulate the photoluminescence properties. Dalton transactions (Cambridge, England : 2003), 50(39).

+ Open protocol

+ Expand

Characterization of Wax Compounds by 13C-NMR

Check if the same lab product or an alternative is used in the 5 most similar protocols

¹³C-NMR spectra in solution were recorded on a Bruker Avance 300-MHz spectrometer (Rheinstetten, Germany) (75.48 MHz for ¹³C) at 373 K with D1 = 2 s. The samples were prepared by introducing 25 mg of the wax in 0.5 mL of 1,1,2,2-tetrachloro-1,2-dideuterioethane (C₂D₂Cl₄) into a tube (5 mm outer diameter). The chemical shifts refer to the central peak of C₂D₂Cl₄ used as internal reference at ∂ = 74.26 ppm.

Lamparelli D.H., Ricca A., Palma V, & Oliva L. (2018). High Conversion of Styrene, Ethylene, and Hydrogen to Linear Monoalkylbenzenes. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(6), 1260.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!