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Axio 10

Manufactured by Zeiss
Sourced in Germany

The AXIO-10 is a high-performance laboratory microscope designed for various scientific applications. It features a sturdy, ergonomic construction and delivers precise optical performance. The AXIO-10 is capable of providing clear, high-resolution images, making it a reliable tool for researchers and scientists.

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5 protocols using axio 10

1

Optical Characterization of Nanostructures

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An optical microscope (Carl-Zeiss Axio-10) equipped with ×5 (0.13 NA), ×10 (0.25 NA), ×20 (0.4 NA), ×50 (0.75 NA), and ×100 (0.85 NA) objective lens is used to obtain the reflection and transmission optical micrographs with different magnifications. The transmittance spectra under normal incidence were collected by an Olympus microscope (BX-51) with a spectrometer through a ×100 objective (MPlan-FLN, 0.9 NA). The extinction spectra were measured in transmission mode using a 2030 PV ultraviolet–visible–near-infrared range microspectrophotometer (CRAIC Technology Inc.) equipped with a xenon light source (80 W) and an optical objective (ZEISS Ultrafluar, ×10, 0.2 NA). Both the incident and collected light were normal to the quartz substrate, thus providing linearly polarized light excitation in plane with the surface of the nanostructures.
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2

Characterizing Metallic Patterns by SEM and Microscopy

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The micrographs of the metallic patterns were obtained by a field-emission SEM (Carl-Zeiss sigma HD) at an acceleration voltage of 10 kV and a working distance of 6 mm. The optical characterization was acquired by a Carl-Zeiss microscope (AXIO-10) equipped with ×5 (0.13 numerical aperture (NA)), ×10 (0.25 NA), ×20 (0.4 NA), ×50 (0.75 NA), and ×100 (0.85 NA) objective lens.
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3

Histological Analysis of Testis and Epididymis

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The fixed tissues were thoroughly washed in 0.01 phosphate buffer (pH 7.4), dehydrated in graded ethanol, toluene-cleared, and embedded in paraffin. Paraffin sections of testis and cauda epididymis were cut at 5 μm, mounted on slides, and stained with hematoxylin and eosin and observed under a light microscope (Nikon 80i; Nikon, Japan).6 (link) The in situ Cell Death Detection horseradish peroxidase Kit (Roche, Mannheim, Germany) was used to carry out the terminal 2′-deoxyuridine 5′-triphosphate nick end-labeling (TUNEL) assay. Briefly, paraffin blocks of testis material were cut into 4-μm thick sections and treated with 20 mg/mL proteinase K solution for 20 minutes at 37°C. The TUNEL reaction was then performed in TdT buffer in the presence of dUTP-biotin for 60 minutes at 37°C and then incubated with the secondary antifluorescein-POD-conjugate for 30 minutes. The signal was visualized using diaminobenzidine. Sections were then counterstained with hematoxylin, dehydrated, cleaned, mounted, and observed under a light microscope (Axio10, Zeiss, Germany).
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4

Optical Characterization of 3D Metasurface Microprints

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For the optical characterisation of the microprints of the 3D-integrated metasurfaces, we used an optical microscope (Carl-Zeiss AXIO-10) with ×5 (0.13NA), ×10 (0.25NA), ×50 (0.75NA), and ×100 (0.85NA) objective lenses. The different magnifications of the devices at each process step were captured with an incoherent white-light source under transmission mode. The microprint display performance was optimised by modifying the exposure time and contrast of the CCD. Then, the fabricated integrated devices were characterised for hologram projection by the experimental setup shown in Fig. S7. The three laser diodes emitting at 450 nm (Thorlabs CPS450), 532 nm (Thorlabs CPS532), and 633 nm (Thorlabs CPS633S) were exploited to generate R, G, and B channels. The polarizers were used to manipulate the source power to achieve a suitable RGB component ratio due to the polarisation-independent property of the proposed metasurfaces. Then, two dichroic lenses (DMLP567T and DMLP490T) were used to combine the three lasers. The hologram images were projected on a screen and captured by an SLR camera. To characterise the diffraction efficiency for each wavelength, we put an optical power metre in position 1 and position 2 as Fig. S7b shows to measure the transmission power and the diffraction power of the hologram image.
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5

Optical Characterization of Microscale Materials

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The transmittance spectra under normal incidence were acquired by Olympus microscope (BX-51) equipped with a spectrometer through a 100 × objective (MPlan-FLN, NA = 0.9). An optical microscope (Carl-Zeiss AXIO-10) with ×5 (0.13 NA), ×10 (0.25 NA), ×20 (0.4 NA), ×50 (0.75 NA), and ×100 (0.85 NA) objective lens is used to obtain the transmission optical micrographs with different magnifications.
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