Log In Sign up
The largest database of trusted experimental protocols

Superose hr6 column

Manufactured by GE Healthcare
Visit Supplier

The Superose HR6 column is a size exclusion chromatography column designed for the separation and purification of proteins, peptides, and other macromolecules. It features a high-resolution (HR) matrix that provides efficient separation over a wide molecular weight range.

Automatically generated - may contain errors

Lab products found in correlation

5973 mass selective detector, by Hewlett-Packard (1 mentions)

2 protocols using superose hr6 column

1

Lipoprotein Fractionation by Size-Exclusion Chromatography

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma lipoproteins were fractionated by size-exclusion chromatography (SEC, Superose HR6 column, GE Health) as previously described [27 (link)]. Cholesterol and TG concentrations of each fraction were determined enzymatically using commercial kits (Wako Diagnostics) and expressed as mg/dL in each fraction or percent of TG and cholesterol of each fraction in total fractions [27 (link)].
Peng X., Lian Z., Perrard X.Y., Xiao Y., Ni J., O’Brien V., Dong H., Pownall H.J., Ballantyne C.M, & Wu H. (2022). Poloxamer 407 Induces Hypertriglyceridemia but Decreases Atherosclerosis in Ldlr−/− Mice. Cells, 11(11), 1795.
+ Open protocol
+ Expand
2

Fatty Acid Profiling and Lipoprotein Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
For FA profiling by GC-MS, plasma samples from mice were hydrolyzed with 0.5% NaOH for 2 h and then delipidated by organic solvent extraction (CHCl3-CH3OH, 2:1, v/v) to yield total hydrolyzed FAs. In addition, split plasma samples were also analyzed by GC-MS for nonesterified FAs (NEFAs). Both total FAs and NEFAs were derivatized with methanol-acetyl chloride (50:1 (v/v)) to yield methylated FAs. FA methyl esters were analyzed by gas chromatography on a fused silica capillary column (Alltech, Deerfield, IL.; 30 m × 0.25 mm × 0.25 μm film). Analysis was performed on a Hewlett-Packard 6890GC coupled to a 5973 Mass Selective Detector as previous described.18 (link), 19 (link) Dietary FA composition was also examined by the above procedure.
Plasma lipoprotein profiles were determined by size exclusion chromatography (SEC, Superose HR6 column; GE Health) of 200 μL plasma per mouse as previously described.20 (link) Cholesterol and TG concentrations of each fraction were determined enzymatically (Wako Diagnostics).
Lian Z., Dai Perrard X.Y., Peng X., Raya J.L., Hernandez A.A., Johnson C.G., Lagor W.R., Pownall H.J., Hoogeveen R.C., Simon S.I., Sacks F.M., Ballantyne C.M, & Wu H. (2019). Replacing saturated fat with unsaturated fat in western diet reduces foamy monocytes and atherosclerosis in male Ldlr–/– mice. Arteriosclerosis, thrombosis, and vascular biology, 40(1), 72-85.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!