Trimethylchlorosilane

Manufactured by Merck Group

Sourced in United States, Germany, Spain, France, China

Trimethylchlorosilane is a colorless liquid used as a reagent in organic synthesis. It is a silylating agent that can be used to protect and functionalize a variety of organic compounds.

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Lab products found in correlation

Pyridine, by Merck Group (24 mentions) Methoxyamine hydrochloride, by Merck Group (12 mentions) N methyl n trimethylsilyl trifluoroacetamide, by Merck Group (9 mentions) Methanol, by Merck Group (9 mentions) Ethanol, by Merck Group (8 mentions) Gallic acid, by Merck Group (6 mentions) Hydrochloric acid (hcl), by Merck Group (6 mentions) Tetracosane, by Merck Group (5 mentions) Anhydrous pyridine, by Merck Group (5 mentions) Hexamethyldisilazane, by Merck Group (5 mentions) Diethyl ether, by Merck Group (5 mentions) Hexadecanoic acid, by Merck Group (4 mentions) Dichloromethane, by Merck Group (4 mentions) Acetonitrile, by Merck Group (4 mentions) Stigmasterol, by Merck Group (4 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) Formic acid, by Merck Group (4 mentions) Chloroform, by Merck Group (4 mentions) Potassium hydroxide, by Merck Group (4 mentions) Nonadecan 1 ol, by Merck Group (3 mentions)

Close spelling variants of this product

Trimethylchlorosilane (TMCS) (41 citations) Bis-[trimethylsilyl]-trifluoroacetamide (BSTFA) + 1 % trimethylchlorosilane (TMCS) (4 citations) Trimethylchlorosilane (BSTFA + 1% TMCS), (2 citations) Trimethylchlorosilane reagent (2 citations)

75 protocols using trimethylchlorosilane

Analytical Procedures for Bioactive Compounds

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Nonadecan-1-ol (99% purity), hexadecanoic acid (≥99% purity), β-stigmasterol (95% purity), vanillin (99%), anhydrous pyridine (99.8% purity), dichloromethane (99% purity), N,O-bis(trimethylsilyl)trifluoroacetamide (99% purity), trimethylchlorosilane (99% purity) and tetracosane (99% purity) were obtained from Sigma Chemicals Co. (Madrid, Spain). Ursolic acid (98% purity) was purchased from Aktin Chemicals (Chengdu, China). Dimethyl sulfoxide (DMSO), cell culture grade, was obtained from PanReac Applichem (Gatersleben, Germany). Acetone (≥99% purity) was supplied by VWR (Radnor, OA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was obtained from Calbiochem (San Diego, CA, USA), and Mueller–Hinton agar or broth was purchased from Liofilchem (Roseto degli Abruzzi, Italy). Brucella Broth was purchased from Fluka Analytical, and Resazurin was obtained from Sigma-Aldrich (St. Louis, MI, USA).

Zazouli S., Chigr M., Ramos P.A., Rosa D., Castro M.M., Jouaiti A., Duarte M.F., Santos S.A, & Silvestre A.J. (2022). Chemical Profile of Lipophilic Fractions of Different Parts of Zizyphus lotus L. by GC-MS and Evaluation of Their Antiproliferative and Antibacterial Activities. Molecules, 27(2), 483.

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Metabolite Profiling of Biological Samples

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All solvents were high purity high-performance liquid chromatography (HPLC) grade purchased from J.T. Baker (Philipsburg, NJ, USA). Distilled water was filtered using a Milli-Q Reagent Water System (Millipore, Billerica, MA, USA). Methyloctanoate, succinic acid-d₄, methoxyamine hydrochloride, pyridine, sodium sulfate, and boron trifluoride-methanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). The certified reference material (CRM), a 37 fatty acid methyl ester mixture, and N-methyl-N-(trimethylsilyl)trifluoroacetamide with trimethylchlorosilane as a silylation reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). The standards of benzeneethanol, benzaldehyde, 1H-pyrrole-2-carboxaldehyde, γ-butyrolactone, γ-caprolactone, δ-hexalactone, γ-octalactone, ethylene glycol, l-(−)-arabitol, d-mannitol, scyllo-inositol, d-glyceric acid sodium salt, fumaric acid, d-psicofuranose, d-gluconic acid solution, and succinic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-Octen-2-one, pantolactone, malic acid, and azelaic acid were obtained from Supelco (Bellefonte, PA, USA).

Kim H., Jung Y., Jeon S.H., Hwang G.S, & Ahn Y.G. (2019). Rapid Characterization and Discovery of Chemical Markers for Discrimination of Xanthii Fructus by Gas Chromatography Coupled to Mass Spectrometry. Molecules, 24(22), 4079.

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Fabricating Microwell Arrays using Soft Photolithography

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The microwell array pattern of the HL-Chip was drawn with AutoCAD software and printed out as glass photomask by Photo Sciences. Mold bearing microwell arrays were fabricated by modified soft photolithography. Briefly, negative photoresists (SU-8 3025, MicroChem) were spin-coated (500 rpm for 10 s and 1,000 rpm–3,000 rpm for 60 s) onto a clean silicon wafer (Silicon Quest International) to form a uniform 20–50 μm film. After baking at 65°C for 3 min and then 95°C for 5 min, the wafer was cooled and exposed to UV light through the glass photomask for 4–10 s. Next, the wafer was baked at 65°C for 3–5 min and then again at 95°C for 8–14 min. The wafer was developed in a SU-8 developer for 4 min and flushed with fresh developer three to four times. The patterned wafer was thoroughly solidified by baking at 135°C for 30 min. Then, the wafer was treated with trimethylchlorosilane (Sigma-Aldrich) for 30 min. A 10:1 (w/w) mixture of PDMS and curing agent (Sylgard 184, Dow Corning) was poured onto the wafer and heated at 70 C for 1 h. Finally, the cured PDMS sheet was cut and peeled off the wafer.

Zhou Y., Shao N., de Castro R.B., Zhang P., Ma Y., Liu X., Huang F., Wang R.F, & Qin L. (2020). Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip. Cell reports, 31(4), 107574.

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Comprehensive Protocol for Microbial Analyses

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Pro and Pep were purchased from the Nutrily Biotechnology, Ltd. (Anyang, Henan, China). Lactobacillus acidophilus JCM 1132 was purchased from the China General Microbiological Culture Collection Center (Beijing, China). Pepsin and trypsin were purchased from the Novozymes (Beijing, China). Bicinchoninic acid (BCA) protein assay kit, acrylamide, glycine, sodium dodecyl sulfate (SDS), Tris, urea and loading buffer were purchased from Solarbio (Beijing, China). Ammonium persulfate and tetramethylethylenediamine (TEMED) were purchased from Amersco (Framingham, USA). Coomassie Brilliant Blue R-250 was purchased from Bio-Rad (Richmond, CA, USA). MRS broth and MRS agar were purchased from Aobox Biotechnology, Ltd. (Beijing, China). Bis trifluoroacetamide (BSTFA), fatty acid methyl ester (FAME), methoxy amination hydrochloride, trimethyl chlorosilane (TMCS), standards of lactic acid, acetic acid, glycine, Gly-Gly-Gly, bacitracin and insulin were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2-Chloro-l-phenylalanine was purchased from Hengbai Biological Technology, Ltd. (Shanghai, China). High performance liquid chromatography (HPLC) grades of acetonitrile, methanol, chloroform, pyridine and trifluoroacetic acid (TFA) were purchased from Fisher Scientific (Ottawa, ON, Canada).

Li W., Zhang Y., Li H., Zhang C., Zhang J., Uddin J, & Liu X. (2020). Effect of soybean oligopeptide on the growth and metabolism of Lactobacillus acidophilus JCM 1132. RSC Advances, 10(28), 16737-16748.

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Okara Metabolite Extraction and Analysis

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All chemicals including nutrient broth, m ethanol, ribitol, methoxamine hydrochloride, N‐methyl‐N‐(trimethylsilyl) trifluoroacetamide (MSTFA), trimethylchlorosilane (TMCS), Folin Ciocalteu's reagent, 20% sodium carbonate, 1‐1,‐diphenyl‐2‐picryl‐hydrazil (DPPH), ethanol, 6‐hydroxy‐2,5,7,8‐tetramethylchroman‐2‐carboxylic acid (Trolox), and gallic acid were purchase from Sigma‐Aldrich. Fresh okara samples were kindly provided by Vitasoy International Singapore Pte Ltd, Singapore. Okara were separated into aliquots and sealed in airtight polyethylene bags and stored at −20°C in the dark.

Mok W.K., Tan Y.X., Lyu X.M, & Chen W.N. (2020). Effects of submerged liquid fermentation of Bacillus subtilis WX‐17 using okara as sole nutrient source on the composition of a potential probiotic beverage. Food Science & Nutrition, 8(7), 3119-3127.

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Extraction and Profiling of Phytochemicals

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All reagents used are HPLC grade. Ultrapure deionized water, with resistivity of 18.2 megaohm-cm was obtained from a Milli-Q^® Integral water purification system with Q-pod (Millipore, Bedford, MA, USA). Formic acid was purchased from Carlo Erba (Milan, Italy). Ethanol, acetonitrile and mEthanol from Merck (Darmstadt, Germany). Ammonium acetate, acetone and 2-propanol were purchased from Carlo Erba (Milan, Italy). The SPE cartridges Waters Oasis HLB (200 mg) from Waters S.p.A. (Milan, Italy). Standard solutions of gallic acid, ascorbic acid, vanillic acid, caffeic acid, chlorogenic acid, catechin, epicatechin, vanillic acid, coumaric acid and hydroxybenzoic acid were purchased from Extrasynthese (Genay Cedex, France); quercetin, rutin, apigenin, luteolin, ferulic acid, kaempferol, palmitic acid, stearic acid, oleic acid, linoleic acid, glucose, N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA), trimethylchlorosilane (TMCS) and pyridine were acquired from Sigma-Aldrich S.r.l. (Milan, Italy).

D’Amico R., Trovato Salinaro A., Fusco R., Cordaro M., Impellizzeri D., Scuto M., Ontario M.L., Lo Dico G., Cuzzocrea S., Di Paola R., Siracusa R, & Calabrese V. (2021). Hericium erinaceus and Coriolus versicolor Modulate Molecular and Biochemical Changes after Traumatic Brain Injury. Antioxidants, 10(6), 898.

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Rhodococcus opacus PD630 Cultivation Protocol

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Furfural, amido black, and acetic acid were purchased from Fisher Scientific (Pittsburgh, PA). 5-Hydroxymethylfurfural (5-HMF), vanillin, ethyl acetate and hexane were purchased from Acros Organics (Morris Plains, NJ). Vanillic acid, trans-p-coumaric acid (TPCA) and cellulase were purchased from Tokyo Chemical Industry Co., LTD (Japan). Glyceryl trioleate (TL), N, O-Bis (trimethylsilyl) trifluoro-acetamide (BSTFA) with trimethylchlorosilane (TMCS), pridine, xylose, glucose, arabinose, o-methoxylamine HCl, acetic anhydride were obtained from Sigma–Aldrich (St. Louis, MO). Glucosidase was purchased from MP Biomedicals (Solon, OH). [1,2- ¹³C₂] _D-glucose was purchased from Cambridge Isotope Laboratories, Inc. (MA, USA). Rhodococcus opacus PD630 (DSM 44193, hereafter referred as strain PD630) was purchased from DSMZ, Germany. The strain was streaked on Reasoner’s 2A (R2A) agar plates for short-term (2–3 weeks) preservation.

Wang B., Rezenom Y.H., Cho K.C., Tran J.L., Lee D.G., Russell D.H., Gill J.J., Young R, & Chu K.H. (2014). Cultivation of lipid-producing bacteria with lignocellulosic biomass: Effects of inhibitory compounds of lignocellulosic hydrolysates. Bioresource technology, 161, 162-170.

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GC/MS and HPLC Analytical Methods

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The GC/MS analyses were performed using, n-hexane, diethyl ether, and isooctane (HPLC grade; Sigma-Aldrich, U.S.). The derivatization was performed using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) added with 1% trimethyl-chlorosilane (Sigma-Aldrich). The tridecanoic acid and hexadecane solutions were prepared in isooctane (140 µg/g and 142 µg/g respectively) using standards with a 99% purity (Sigma-Aldrich).
The HPLC analyses were performed using n-hexane, iso-propanol, chloroform and methanol (HPLC-MS grade; Fluka, U.S.).

Lucejko J.J., La Nasa J., Porta F., Vanzetti A., Tanda G., Mangiaracina C.F., Corretti A., Colombini M.P, & Ribechini E. (2018). Long-lasting ergot lipids as new biomarkers for assessing the presence of cereals and cereal products in archaeological vessels. Scientific Reports, 8, 3935.

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Dexamethasone Metabolite Analysis Protocol

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Dexamethasone was purchased from Merck Serono, Galaxo SmithKline, UK. Glacial acetic acid, methoxyamine hydrochloride, pyridine, n-methyl-n-(trimethylsilyl) trifluoroacetamide (MSTFA), trimethylchlorosilane, acetonitrile (99.8%) and n-alkane C8-C40 standards were purchased from Sigma-Aldrich (St. Louis, MO, USA). Silver nitrate (AgNO₃) was purchased from Merck (Burlington, VT, USA).

Abdallah H.M., Ammar N.M., Abdelhameed M.F., Gendy A.E., Ragab T.I., Abd-ElGawad A.M., Farag M.A., Alwahibi M.S, & Elshamy A.I. (2020). Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations against Ulcerative Colitis in Rats as Revealed via Biochemical and Metabolomic Assays. Biology, 9(8), 195.

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Methoxyamine Derivatization and GC-MS Analysis

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The derivatization process involved addition of 25 μL solution consisting of 20 mg/mL methoxyamine hydrochloride in pyridine, followed by constant shaking for 60 min at 50 °C and the addition of MSTFA (N-Methyl-N-(trimethylsilyl)trifluoroacetamide, Sigma Aldrich, Steinheim, Germany) + 1% TMCS (Trimethylchlorosilane, Sigma Aldrich, Steinheim, Germany), with incubation at 50 °C for 30 min for complete derivatization. The sample was dissolved in 100 μL pyridine.
The samples were analyzed using an Agilent 8890 GC system coupled with triple quad spectrometer system MS 7000D GC/TQ. The column was HP-5 MS (30m × 0.25 mm × 0.25 μm, Agilent, Santa Clara, CA, USA) with an oven program set on 60 °C maintained for 2 min, then increased to 210 °C at rate of 10 °C/min, ramped to 240 °C at rate of 5 °C/min and then ramped to 315 °C at a rate of 25 °C/min, and then held at 315 °C for 3 min.

Pervan M., Marijan S., Markotić A., Pilkington L.I., Haverkate N.A., Barker D., Reynisson J., Meić L., Radan M, & Čikeš Čulić V. (2022). Novel Thieno [2,3-b]pyridine Anticancer Compound Lowers Cancer Stem Cell Fraction Inducing Shift of Lipid to Glucose Metabolism. International Journal of Molecular Sciences, 23(19), 11457.

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