Cells were counted using the TC10™ automated cell counter from BioRad (BioRad, Hercules, CA) and equal number of viable cells was plated in 96 well plates. To measure cell proliferation, the MTS-based cell proliferation assay kit was used (Promega, Madison, WI). The assay was performed every 24 hrs and incubation time was kept constant. The intensity of the chromogenic substrate was measured at 490 nm.
Mts based cell proliferation assay kit
Manufactured by Promega
Sourced in United States
The MTS-based cell proliferation assay kit is a product that allows for the colorimetric quantification of cell viability and proliferation. The assay is based on the conversion of the tetrazolium compound MTS into a colored formazan product by metabolically active cells. The amount of formazan produced is directly proportional to the number of living cells in the sample, providing a simple and reliable method for measuring cell proliferation.
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3 protocols using mts based cell proliferation assay kit
1
Cell Proliferation Assay Protocol
2
Cell Proliferation Assay of HTR-8/SVneo cells
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HTR-8/SVneo cells were seeded in 96-well plates at a density of 2000 cells/well. The next day, the attached cells were treated with siRNA. The cell numbers were determined at 24, 48, and 72 h after treatment using a [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS]-based cell proliferation assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions.
3
Cell Proliferation Assay Protocol
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