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Pisre reporter plasmid

Manufactured by Agilent Technologies
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The PISRE reporter plasmid is a laboratory tool designed for gene expression analysis. It contains a promoter element that drives the expression of a reporter gene, which can be used to monitor and quantify gene expression levels in various experimental settings. The core function of this plasmid is to provide a standardized platform for researchers to study gene regulation and activity.

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Lab products found in correlation

Human ifn β 1a, by PBL Assay Science (2 mentions) Transit lt1, by Mirus Bio (1 mentions)

2 protocols using pisre reporter plasmid

1

Assessing ISGF3 Activation by VP24

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293 cells were seeded in 12-well plates 1 day before transfection for 30–50% confluency at the time of transfection. Transfection was performed using TransIT-LT1 according to the manufacturer's instructions. Plasmids transfected included 250 ng firefly luciferase in a pISRE reporter plasmid (Agilent)47 (link), 50 ng pCAGGS-hrluc for normalization and 1–100 ng (in threefold dilutions) pCAGGS-VP24, with wells receiving <100 ng VP24 balanced by empty vector. Twenty-four hours later, 1,000 IU human IFN-β 1a (PBL Assay Science) were added. Cells were harvested and reporter activity was measured 8 h after IFN application as described for the IFN production assay.
Dunham E.C., Banadyga L., Groseth A., Chiramel A.I., Best S.M., Ebihara H., Feldmann H, & Hoenen T. (2015). Assessing the contribution of interferon antagonism to the virulence of West African Ebola viruses. Nature Communications, 6, 8000.
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2

Evaluating VP24 Modulation of IFN-β Signaling

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293 cells were seeded in 12-well plates one day before transfection for 30–50% confluency at the time of transfection. Transfection was performed using TransIT-LT1 (Mirus) according to the manufacturer’s instructions. Plasmids transfected included 250 ng firefly luciferase in a pISRE reporter plasmid (Agilent) 47 (link), 50 ng pCAGGS-hrluc for normalization, and 1–100 ng (in three-fold dilutions) pCAGGS-VP24, with wells receiving less than 100 ng VP24 balanced by empty vector. Twenty-four hours later, 1000 I.U. human IFN-β 1a (PBL Assay Science) was added. Cells were harvested and reporter activity measured 8 hours after IFN application as described for the IFN production assay.
Dunham E.C., Banadyga L., Groseth A., Chiramel A.I., Best S.M., Ebihara H., Feldmann H, & Hoenen T. (2015). Assessing the contribution of interferon antagonism to the virulence of West African Ebola viruses. Nature Communications, 6, 8000.
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