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Anti cspg4 clone 1e6

Manufactured by Miltenyi Biotec
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The Anti-CSPG4 (Clone 1E6.4) is a monoclonal antibody product designed for research use. It recognizes the Chondroitin Sulfate Proteoglycan 4 (CSPG4) antigen. This antibody can be used for the detection and analysis of CSPG4 expression in various experimental systems.

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Lab products found in correlation

Cd62l, by BD (2 mentions) Anti gd2 clone 14 g2a, by BD (2 mentions) Facscalibur system, by BD (2 mentions) 7 aad, by BD (2 mentions) Annexin 5, by BD (2 mentions) Cellquest software, by BD (2 mentions)

2 protocols using anti cspg4 clone 1e6

1

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed flow cytometry analysis using the following Abs: CD45, CD56, CD8, CD4, CD3, CD45RA, CD45RO, CD62L, 7AAD and Annexin V (all from Becton Dickinson, San Jose, CA) and CCR7 (from E&D) conjugated with FITC, PE, PerCP or APC fluorochromes. Expression of GD2 and CSPG4 antigens on tumor cell lines was assesed with anti-GD2 (clone 14.g2a, BD) and anti-CSPG4 (Clone 1E6.4, Miltenyi-Biotech), respectively. The expression of GD2-specific CAR was detected using a specific anti-idiotype antibody (1A7). Samples were analyzed with a BD FACScalibur system equipped with the filter set for quadruple fluorescence signals and the CellQuest software (BD Biosciences). For each sample we analyzed a minimum of 10,000 events.
Caruana I., Savoldo B., Hoyos V., Weber G., Liu H., Kim E.S., Ittmann M.M., Marchetti D, & Dotti G. (2015). Heparanase promotes tumor infiltration and antitumor activity of CAR-redirected T-lymphocytes. Nature medicine, 21(5), 524-529.
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2

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed flow cytometry analysis using the following Abs: CD45, CD56, CD8, CD4, CD3, CD45RA, CD45RO, CD62L, 7AAD and Annexin V (all from Becton Dickinson, San Jose, CA) and CCR7 (from E&D) conjugated with FITC, PE, PerCP or APC fluorochromes. Expression of GD2 and CSPG4 antigens on tumor cell lines was assesed with anti-GD2 (clone 14.g2a, BD) and anti-CSPG4 (Clone 1E6.4, Miltenyi-Biotech), respectively. The expression of GD2-specific CAR was detected using a specific anti-idiotype antibody (1A7). Samples were analyzed with a BD FACScalibur system equipped with the filter set for quadruple fluorescence signals and the CellQuest software (BD Biosciences). For each sample we analyzed a minimum of 10,000 events.
Caruana I., Savoldo B., Hoyos V., Weber G., Liu H., Kim E.S., Ittmann M.M., Marchetti D, & Dotti G. (2015). Heparanase promotes tumor infiltration and antitumor activity of CAR-redirected T-lymphocytes. Nature medicine, 21(5), 524-529.
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