Macrophage colony stimulating factor (M-CSF) and receptor activator of NF-KB ligand (RANKL) factor were obtained from Sigma (St Louis, MO). Titanium particle was supplied by Nonferrous Metals Company (Beijing, China) with a mean diameter of 91 ± 15 μm. Trizol was purchased from Invitrogen (Carlsbad, CA). RANK ELISA kit was obtained from Xitang Biology Sci-tech Co., Ltd (Shanghai, China). The primers for tartrate-resistant acid phosphatase (TRAP), CA II, matrix metalloproteinase 9 (MMP-9), Cathepsin K (CtsK) and GAPDH were supplied by Sangon Biological Engineering Technology & Services Co., Ltd (Shanghai, China). The cDNA Synthesis and SYBR Premix Ex Taq polymerase chain reaction (PCR) Kits were purchased from TaKaRa (Shiga, Japan). The antibodies for TRAP, RANK, CtsK and β-actin were purchased from Cell Signaling Technology (Beverly, MA). GeneAmp PCR System 9600 was purchased from Perkin Elmer. The 7500 Real-Time PCR Systems was supplied by Applied Biosystems. Finally, the TRAP Staining Kit was obtained from Jian Cheng Bioengineering Institute (Nanjing, China).
Trap staining kit
Manufactured by Nanjing Jiancheng
Sourced in China
The TRAP staining kit is a laboratory product used to detect and visualize the presence of Tartrate-Resistant Acid Phosphatase (TRAP) enzyme activity in biological samples. The kit provides the necessary reagents and protocols to perform this specific staining technique.
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Lab products found in correlation
3 protocols using trap staining kit
1
Titanium Particle-Induced Osteoclastogenesis
2
Quantitative Analysis of Osteoclast Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For TRAP staining, cells were rst washed three times with PBS after the culture medium was removed and xed in 4% paraformaldehyde for 15min, followed by stained using a TRAP staining kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturers' instructions. After 30 min, staining solution was washed off and the TRAP activity was analyzed by colorimetry. At the same time, the number and size of multinucleated (more than three nuclei) osteoclasts were analyzed and calculated by the imageJ software. TRAP staining for para n-embedded tissue sections was carried out by Runnerbio biotech. Comp (Shanghai, China) with similar methods. N.Oc/BS (number of osteoclast per bone surface) and ES/BS (eroded surface per bone surface) were used to measure the number and size of osteoclasts in the mouse bone marrow.
3
Quantitative Analysis of Osteoclast Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For TRAP staining, cells were rst washed three times with PBS after the culture medium was removed and xed in 4% paraformaldehyde for 15min, followed by stained using a TRAP staining kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturers' instructions. After 30 min, staining solution was washed off and the TRAP activity was analyzed by colorimetry. At the same time, the number and size of multinucleated (more than three nuclei) osteoclasts were analyzed and calculated by the imageJ software. TRAP staining for para n-embedded tissue sections was carried out by Runnerbio biotech. Comp (Shanghai, China) with similar methods. N.Oc/BS (number of osteoclast per bone surface) and ES/BS (eroded surface per bone surface) were used to measure the number and size of osteoclasts in the mouse bone marrow.
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