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3 protocols using poly styrene co maleic anhydride psma

1

Polymer-based Cell Internalization Study

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Poly (styrene‐co‐maleic anhydride) (PSMA, Mn = 1700) and anhydrous tetrahydrofuran (THF, 99.9%) were purchased from Sigma‐Aldrich. Poly [(9,9‐dioctylfluorenyl‐2,7‐diyl)‐co‐(1,4‐benzo‐{2,1,3}‐thiadiazole)] (PFBT, MW = 10 000, polydispersity 1.7) was obtained from ADS Dyes (Quebec, Canada). Octaarginine peptides were purchased from Jie Li Bio. HeLa cell lines were purchased from Cell Bank of Chinese Academy of Sciences (Shanghai). Minimum essential media (MEM), Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were from Gibco, Invitrogen. Chlorpromazine (CPZ), methyl‐β‐cyclodextrin (mβCD) and EIPA were purchased from Sigma‐Aldrich (St. Louis, MO, USA). RFP‐LAMP1 plasmid was acquired from Addgene (plasmid # 1817).
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2

Preparation of Melamine-PFO-PSMA Solution

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Melamine was purchased from Dr. Ehrensorfer (Hamburg, Germany) and dissolved in methanol to prepare a stock solution (1 mM) for further use. Poly (9,9-dioctylfluorenyl-2,7-diyl) (PFO, average MW = 58 200, polydispersity = 3.7), and poly (styrene-co-maleic anhydride) (PSMA, average MW = 1700, styrene content 68%) were purchased from Sigma-Aldrich (St. Louis, USA). Tetrahydrofuran (THF, anhydrous, 99.9%) and other reagents were all analytical grade, and supplied by Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Ultrapure water (18.2 MΩ.cm) was prepared with a Milli-Q system (Millipore, Bedford, MA, USA) and used throughout the study.
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3

Synthesis and Characterization of Fluorescent Polymer Nanoparticles

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Poly (9,9-dioctylfluorene-2,7-diyl) (PFO) and poly (styrene-co-maleic anhydride) (PSMA) were purchased from Sigma-Aldrich. Tetrahydrofuran (THF; anhydrous, ≥99.9%, inhibitor-free), diphenylcarbazide (DPC), K2Cr2O7, NaCl, MgCl2, KCl, CaCl2, FeCl3 6H2O, CuCl2 2H2O, NiCl2, KMnO4, acetic acid, boric acid, phosphoric acid, HCl and NaOH were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shangai, China).
The apparatus used in the work included a transmission electron microscope (Talos F200X G2, Thermo Fisher, Waltam, MA, USA); dynamic light scattering system (DLS, Malvern Zetasizer Nano ZS, Malvern, United Kingdom); U-3900 ultraviolet–visible (UV–vis) spectrophotometer (Hitachi, Japan); F-7000 fluorescence spectrophotometer (Hitachi, Japan) and Laser Scanning Confocal Microscopy (Carl Zeiss, Germany).
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