Cmv cre

All animal procedures were approved by the Institutional Animal Care and Use Committees of The Scripps Research Institute (TSRI). All animals were housed in the same vivarium conditions at a constant temperature of 20^oC, with a 12 hour light/dark cycle.
Constitutive, RBC-specific, and macrophage specific GOF Piezo1 mice were generated by breeding mice with conditional GOF Piezo1 allele with Cmv-cre (The Jackson Laboratory stock# 006054), EpoR-cre (a gift from Dr. Klingmuller group at Max-Planck-Institute für Immunbiologie, Freiburg, Germany), and LysM-cre (The Jackson Laboratory stock# 004781). All GOF Piezo1 mice were generated at Taconic, Hudson, NY, and maintained on C57BL/6 background (Ma et al., 2018 ). Heterozygous GOF mice were used for the experiments, unless mentioned otherwise. Macrophage-specific LOF Piezo1 mice were generated by breeding mice with conditional LOF Piezo1 allele (The Jackson Laboratory stock# 029213) with the same LysM-cre. All cre driver mice were on C57BL/6 background or were backcrossed at least 10 generations to C57BL/6. Littermates with a mix of equal number of male and female mice were used for experiments. Aging mice (12months to 18months old) born from same breeders were used in the experiments.

WT C57BL/6J (stock no. 000664), CMV-cre (stock no. 006054), Itgax-cre (stock no. 008068) and Vav-cre (stock no. 008610) mice were obtained from the Jackson Laboratory. B6-Ly5.1/Cr mice (strain code 564) were obtained from Charles River. Irf8 +32^−/− mice (stock no. 032744; The Jackson Laboratory) were generated in house and described previously^{6 (link)}. Mice harbouring floxed alleles of Nfil3 (Nfil3^fl/fl mice) and Cebpb (Cebpb^f/fl mice) were described previously^{46 (link),47 (link)}. Nfil3^−/− mice were provided by A. Look and T. Mak^{48 (link)}. All mice were maintained on the C57BL/6J background in our specific-pathogen free facility following institutional guidelines and with protocols approved by the AAALAC-accredited Animal Studies Committee at Washington University in St Louis. All animals were maintained on 12-h light cycles and housed at 21 °C and 50% humidity. Experiments were performed with mice at 6–12 weeks of age, with sex-matched littermates whenever possible.

The C57BL/6J and ob/ob mice were purchased from The Jackson Laboratory, Bar Harbor, ME, all were male aged at 6–8 weeks.
miR-128-1 KO mice: experimental manipulation of miR-128-1 in vivo was achieved by crossing mice carrying miR-128-1 floxed alleles (Tan et al., 2013 (link)) to transgenic Cre-recombinase mouse lines. For germline excision, we used the ubiquitously expressed cytomegalovirus-Cre mouse line (CMV-Cre; Jackson Labs #006054). Genotyping was performed as described in Wark et al., (2017) (link). Lines were maintained on the C57BL/6J background on a 12-hour light-dark cycle with constant access to food and water. Male mice with age from 6 weeks to 16 weeks were used in different experiments. All experimental procedures were conducted in accordance with IACUC regulations and were approved by Massachusetts General Hospital’s or Yale University School of Medicine’s IACUC.