Earle s salts

The African monkey Vero cells (ATCC^® CCL-81™) were maintained in MEM with Earle’s Salts (Capricorn Scientific, Ebsdorfergrund, Germany) supplemented with 10% fetal calf serum (FCS), L-glutamine and penicillin/streptomycin (10 μg/mL) as well as non-essential amino acids (NEAA). Culture was maintained at 37C with 5% CO₂. The human B cells, Raji (ATCC^® CCL-86™) as well as Raji transfectants stably expressing human DC-SIGN or human langerin created by electroporation (40 (link)) were cultured in RPMI 1640 medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS), penicillin/streptomycin (10 μg/mL). The expression of DC-SIGN and langerin was regularly checked via FACS analysis.

Vero cells (clone E6, CCLV-RIE-929/25) were cultivated in MEM with Earle’s salts (Capricorn Scientific, Ebsdorfergrund, Germany) supplemented with 2 mM stable L-glutamine (Capricorn Scientific), 100 U/mL penicillin, 100 µg/mL streptomycin (Capricorn Scientific), and 10% fetal bovine serum (FBS) (Capricorn Scientific) at 37 °C in 5% CO₂. C6/36 (CCLV-RIE-1299) cells were grown in Schneiders Drosophila media (PAN-Biotech, Aidenbach, Germany) supplemented with 2 mM stable L-glutamine (Capricorn Scientific), 1 mM sodium pyruvate (PAN Biotech), 1X MEM NEAA (PAN Biotech), 100 U/mL penicillin, 100 µg/mL streptomycin (Capricorn Scientific), and 10% FBS (Biowest, Riverside, MO, USA) at 28 °C. The suspension cell line FreeStyle™ CHO-S (Thermo Fisher Scientific, Waltham, MA, USA) was cultivated in FreeStyle^TM CHO medium (Thermo Fisher Scientific) supplemented with 8 mM stable L-glutamine (Capricorn Scientific), 100 U/mL penicillin, 100 µg/mL streptomycin (Capricorn Scientific) at 37 °C, and 5% CO₂ on an orbital shaker.