Indole 3 carbinol

Manufactured by Merck Group

Sourced in United States, Germany

Indole-3-carbinol is a chemical compound that is isolated from cruciferous vegetables. It is commonly used as a laboratory reagent for various applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Indole (92 citations)

26 protocols using indole 3 carbinol

Modulation of GPR15 Expression by AhR Ligands

Check if the same lab product or an alternative is used in the 5 most similar protocols

A suspension of Indole-3-carbinol (I3C; Sigma-Aldrich, St. Louis, MI) was prepared in corn oil (100 mg/mL). AHR wildtype, Gpr15^gfp/,+ mice were orally gavaged with 600 mg/kg (≈150 μL per 20 g mouse) once daily, five times a week for 3 weeks. In separate studies, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; purchased from AccuStandard, New Haven, CT) was dissolved in DMSO to a stock concentration of 5 μg/mL. Gpr15^gfp/,+ mice were i.p. injected with 1 μg/kg or 10 μg/kg (or 40 μL per 20 g mouse) three times a week (every other day) for 3 weeks. Expression of GPR15 on tissue lymphocytes was assessed by flow cytometry. In separate experiments, GPR15-sufficient Ahr^+/+ (CD45.1) and Ahr^−/− (CD45.2) treated with DMSO or 10 μg/kg of TCDD and expression of GPR15 on tissue lymphocytes was analyzed using GPR15-PE antibody (Biolegend).

Swaminathan G., Nguyen L.P., Namkoong H., Pan J., Haileselassie Y., Patel A., Ji A.R., Mikhail D.M., Dinh T.T., Singh H., Liao B., Vázquez-Montesino L.M., Butcher E.C, & Habtezion A. (2021). The aryl hydrocarbon receptor regulates expression of mucosal trafficking receptor GPR15. Mucosal Immunology, 14(4), 852-861.

+ Open protocol

+ Expand

Dosing of AhR Ligands in Mice

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

2,3,7,8,Tetrachlorodibenzo-p-dioxin (dioxin, TCDD) was obtained from Cambridge Isotopes (Cambridge, MA). A 1 mg/mL stock solution of TCDD in anisole/peanut oil was diluted to yield treatment solutions containing 1 or 10 μg/ mL in peanut oil. 2-(1H-Indol-3-ylcarbonyl)-4-thiazolecar- boxylic acid methyl ester (ITE) was obtained from Tocris (Bio-techne brand, Minneapolis, MN). A 20 mg/mL stock solution of ITE in DMSO was diluted to yield a treatment solution containing 0.8 mg/mL in peanut oil. Indole 3-car- binol (I3C), obtained from Sigma-Aldrich (St. Louis, MO), was suspended in peanut oil (10 mg/mL). The estimated half-life of TCDD is approximately 10 days in mice (Birn-baum 1986 (link); Gasiewicz et al. 1983 (link)), whereas the in vivo absorption, metabolism, distribution, and excretion rates of ITE and I3C in mice remain largely undetermined. There fore, the selected doses and routes of exposure for these AhR ligands were based upon previous reports, thus integrating our results with previous findings in other model systems (Abron et al. 2018 (link); Benson and Shepherd 2011a (link); Boule et al. 2018b (link); Nugent et al. 2013 (link); Quintana et al. 2010b (link); Singh et al. 2016 (link)).

Beamer C.A., Kreitinger J.M., Cole S.L, & Shepherd D.M. (2018). Targeted deletion of the aryl hydrocarbon receptor in dendritic cells prevents thymic atrophy in response to dioxin. Archives of toxicology, 93(2), 355-368.

+ Open protocol

+ Expand

Neuroprotective Effects of PTEN Inhibition

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nicotine, methyllycaconitine (MLA), a selective α7 nAChR blocker (Gowayed et al., 2019 (link); Papke and Horenstein, 2021 (link)), and a potent PTEN protector (Lee et al., 2019 (link)), indole-3-carbinol (I3C), were purchased from Sigma-Aldrich. The inhibitor of PTEN, SF1670, was purchased from Selleckchem. SF1670 and I3C were dissolved in dimethylsulfoxide (DMSO) first and diluted in saline solution, while nicotine and MLA were dissolved in saline solution directly. Rats had already received intraperitoneal injections of nicotine at 1 mg/kg and MLA at 1 mg/kg once daily for 1 week before surgery and continued receiving intraperitoneal injections of 1 mg/kg nicotine or MLA in sterile saline once daily for 3 week (Teng et al., 2019 (link)), whereas matched control rats received an equal volume of intraperitoneal saline. SF1670 (0.3 mg/kg; Li et al., 2011 (link)) and I3C (2 mg/kg; Lee et al., 2019 (link)) were injected intraperitoneally in nicotine-treated rats simultaneously until the date of surgery to observe the nociceptive effect of PTEN deficiency and analgesic effects for the prevention of PTEN degradation, respectively.

Fang Y., Zhang T., Li L., Chen S., Wang L., Tang J, & Liao Y. (2023). Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models. eNeuro, 10(9), ENEURO.0185-23.2023.

+ Open protocol

+ Expand

Comprehensive Biochemical Protocol Compendium

Check if the same lab product or an alternative is used in the 5 most similar protocols

DMBA (7, 12- dimethylbenz(α)anthracene), Indole 3 carbinol (I3C), 5-5′dithiobisnitrobenzoic acid (DTNB) sodium dithionate and malondialdehyde were obtained from Sigma-aldrich, Banglore, India. Xylenol orange, ammonium ferrous sulfate, cyclohexane, 2,6-dichlorophenol-indophenol (DCPIP), reduced nicotinamide adenine dinucleotide (NADH), reduced nicotinamide adenine dinucleotide phosphate (NADPH), bovine serum albumin (BSA), potassium ferricyanide, 1-chloro-2,4-dinitrobenzene (CDNB), glycylglycine, reduced glutathione (GSH), oxidized glutathione (GSSG), ascorbic acid, guiacol, triton X, nitroblue tetrazolium chloride (NBT), hydroxylamine hydrochloride, pyruvate were obtained from Himedia Laboratories, Mumbai, India. All other reagents used in the present study were of analytical grade.

Kumar R., Kaur R., Singh A.P, & Arora S. (2014). Diminution of Hepatic Response to 7, 12-dimethylbenz(α)anthracene by Ethyl Acetate Fraction of Acacia catechu Willd. through Modulation of Xenobiotic and Anti-Oxidative Enzymes in Rats. PLoS ONE, 9(2), e90083.

+ Open protocol

+ Expand

I3C Solubility and Dosing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Indole-3-carbinol (I3C) was obtained from Sigma-Aldrich (Product Number: 17256, CAS-No.: 700-06-1). For in vitro assays, I3C was dissolved in 100% DMSO and added to the cell culture medium at different concentrations. For in vivo assay, the corresponding dose of I3C suspension is prepared using 10% DMSO, and the solvent was 0.9% sodium chloride solution.

Centofanti F., Alonzi T., Latini A., Spitalieri P., Murdocca M., Chen X., Cui W., Shang Q., Goletti D., Shi Y., Duranti A., Tomino C., Biancolella M., Sangiuolo F., Capobianchi M.R., Jain S., Novelli G, & Pandolfi P.P. (2022). Indole-3-carbinol in vitro antiviral activity against SARS-Cov-2 virus and in vivo toxicity. Cell Death Discovery, 8, 491.

+ Open protocol

+ Expand

Culturing HepG2 Liver Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human liver cancer HepG2 cells were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Beijing, China) and cultured in DMEM (C11995500BT, Gibco, Waltham, MA, USA) containing 10% fetal bovine serum (FBS, 04-001-1A, BI, Herzliya, Israel) and 1% penicillin and streptomycin (P1400, Solarbio, Beijing, China) under 37 °C and humidified 5% CO₂. Indole-3-carbinol (I3C), dissolved in dimethyl sulfoxide (DMSO, D5879, Sigma-Aldrich, St. Louis, MO, USA) was purchased from Sigma-Aldrich (I7256, St. Louis, MO, USA). Pifithrin-α (PFT-α, S1816) was purchased from Beyotime Biotechnology (Shanghai, China).

Qi Y., Zhang C., Wu D., Zhang Y., Zhao Y, & Li W. (2022). Indole-3-Carbinol Stabilizes p53 to Induce miR-34a, Which Targets LDHA to Block Aerobic Glycolysis in Liver Cancer Cells. Pharmaceuticals, 15(10), 1257.

+ Open protocol

+ Expand

Analytical Protocols for Botanical Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Imidazol formate, formic acid, acetonitrile, sulfatase, 2-propanol, 2,2-azinobis-(ethyl-2,3-dihydrobenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,2-benzenedithiol, 3,3′-diindolylmethane (DIM), and indole-3-carbinol (I3C) were obtained from Sigma-Aldrich (Darmstadt, Germany). Glucotropaeolin (GTL) was from AppliChem (Darmstadt, Germany), indole-3-acetonitrile (I3ACN) from Merck (Darmstadt, Germany), and HPLC grade methanol from Chempur (Piekary Śląskie, Poland). Water was purified with a QPLUS185 system from Millipore (Burlington, MA, USA).

Kapusta-Duch J, & Kusznierewicz B. (2021). Young Shoots of White and Red Headed Cabbages Like Novel Sources of Glucosinolates as Well as Antioxidative Substances. Antioxidants, 10(8), 1277.

+ Open protocol

+ Expand

Evaluating Dioxin Receptor Modulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxine), skatole (3-methylindole), indole-3-carbinol (I3C), 1-aminobenzotriazole (ABT) and actinomycin D were from Sigma-Aldrich (Saint-Louis, MO, US). CH-223191 (2-Methyl-2H-pyrazole-3-carboxylic acid-(2-methyl-4-o-tolyl-azophenyl)-amide, an antagonist of TCDD-mediated AhR activation [24 (link), 25 (link)], was from Calbiochem (Merck KGaA, Damstadt, Germany).

Rasmussen M.K., Balaguer P., Ekstrand B., Daujat-Chavanieu M, & Gerbal-Chaloin S. (2016). Skatole (3-Methylindole) Is a Partial Aryl Hydrocarbon Receptor Agonist and Induces CYP1A1/2 and CYP1B1 Expression in Primary Human Hepatocytes. PLoS ONE, 11(5), e0154629.

+ Open protocol

+ Expand

Dissolution and Storage of I3C, DIM, and 5-FU

Check if the same lab product or an alternative is used in the 5 most similar protocols

Indole-3-carbinol (I3C), 3,3-diindolylmethane (DIM), and 5-Fluorouracil (5-FU) were all acquired from Sigma-Aldrich (Saint Louis, Mo, USA); they were dissolved in a mixture of dimethylsulfoxide (DMSO) and corn oil and stored at 4°C in the dark. Immediately before use, I3C, DIM, and 5-FU were dissolved in physiological saline to the required nal concentration used for treatment.

Salem A.Z., Medhat D., Fathy S.A., Mohamed M.R., El-Khayat Z., & El‐Daly S.M. (2021). Indole Glucosinolates Exhibits Anti-Inflammatory Effect on Ehrlich Ascites Carcinoma Cells through Modulation of Inflammatory Markers and miRNAs.

+ Open protocol

+ Expand

Phytochemical Screening and Antioxidant Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following chemicals were used: methanol, acetonitrile, isopropanol (HPLC grade, Merck, Darmstadt, Germany), formic acid (>96.0%), 2,2-azinobis-(ethyl-2,3-dihydrobenzothiazoline-6-sulphonic acid) diammonium salt-ABTS, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid-Trolox, caffeic acid, ferulic acid, sinapic acid, quercetin, kaempferol, indole-3-carbinol, indole-3-acetonitrile, 3,3′-diindolylmethane, N-acetyl-l-cysteine, allyl isothiocyanate, sulforaphane (Sigma Aldrich, St. Louis, MO, USA), and glucotropaeolin (AppliChem, Darmstadt, Germany).

Seljåsen R., Kusznierewicz B., Bartoszek A., Mølmann J, & Vågen I.M. (2022). Effects of Post-Harvest Elicitor Treatments with Ultrasound, UV- and Photosynthetic Active Radiation on Polyphenols, Glucosinolates and Antioxidant Activity in a Waste Fraction of White Cabbage (Brassica oleracea var. capitata). Molecules, 27(16), 5256.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!