Cell migration and invasion were performed by using BioCoat Control Cell Culture Inserts and BioCoat Growth Factor Reduced Matrigel Invasion Chambers (BD Biosciences), respectively. For migration assays, 5 × 104 cells were plated in the upper chamber with the uncoated membrane (24-well insert; pore size, 8 µm). For invasion assays, 1.25 × 105 cells were plated in the upper chamber with Matrigel-coated membrane (24-well insert; pore size, 8 µm). In both assays, cells were plated in medium without serum or growth factors, and complete medium (serum containing) was used as a chemoattractant in the lower chamber. After incubation for 20 h, cells that did not migrate or invade through the pores were removed by a cotton swab. Cells on the lower surface of the membrane were fixed with 4% paraformaldehyde, stained with 0.5% crystal violet, and microscopically counted from three random fields of each membrane. The average cell number per field for triplicate membranes was used to calculate the mean with SD.
Biocoat control cell culture inserts
Manufactured by BD
Sourced in United States
The BD BioCoat Control Cell Culture Inserts are a cell culture product designed for in vitro cell studies. The inserts provide a physical barrier that separates two compartments within a cell culture system.
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Lab products found in correlation
12 protocols using biocoat control cell culture inserts
1
Cell Migration and Invasion Assay
2
Assessing Cell Migration and Invasion
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BioCoat Matrigel invasion chambers, 24-well plates (BD Biosciences) were utilised. For the cell migration assay and the invasion assay, 5 × 103 and 2.5 × 103 cells, respectively, were seeded onto the porous membranes in upper chambers of BioCoat control cell-culture inserts (8.0 μm pore size; BD Biosciences) after treatment for 7 days with eribulin or 5-FU. Top chambers (culture inserts) were filled with serum-free medium, and bottom chambers were filled with medium containing 20% FBS as a chemoattractant. After 16 h incubation for migration or 26 h incubation for invasion, the number of cells that had migrated to bottom surfaces of the membranes was counted after staining with Geimsa solution. Numbers of cells in wells were calculated using means from five randomly selected fields.
3
Cell Migration and Invasion Assay
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The cell migration and invasion assay was analyzed with BD BioCoat Control Cell Culture Inserts and BD BioCoat Matrigel Invasion Chamber (both BD Biosciences), according to the manufacturer’s instructions. After 48 h incubation, cells were wiped off from the upper surface of the insert. Cells on the lower surface were fixed with methanol, stained with hematoxylin-eosin, and counted by microscope.
4
Transwell Assay for Cell Migration and Invasion
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Transwell assays were performed to assess cell migration and invasion using BD BioCoat Control Cell Culture Inserts or the BD BioCoat BD MatrigelTM Invasion Chamber (BD Biosciences, San Jose, CA, USA). In brief, cells were seeded in the upper Boyden chambers of the cell culture inserts. After 24 h of incubation, cells remaining in the upper chamber were carefully removed. Cells adhering to the lower membrane were stained with DAPI in the dark, and then imaged and counted using an inverted microscope equipped with the Zeiss Image digital camera. Three random fields were captured at 200× magnification. The number of cells on the bottom surface was compared between the two groups.
5
Transwell Assays for Cell Migration and Invasion
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Transwell assays were performed to assess cell migration and invasion using BD BioCoat Control Cell Culture Inserts and BD BioCoat BD MatrigelTM Invasion Chambers, respectively [30 (link)]. In brief, cells were seeded in the upper Boyden chambers of the cell culture inserts. After a 24 h incubation, cells that had adhered to the lower membrane were stained with 4’,6-diamidino-2-phenylindolein the dark, imaged and counted. Three random fields were captured at 200× magnification under a microscope. The number of cells on the bottom surface was compared among the groups.
6
Transwell Assay for Cell Migration and Invasion
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Transwell assays were performed to assess cell migratory and invasion capacities using BD BioCoat Control Cell Culture Inserts and BD BioCoat BD MatrigelTM Invasion Chambers, respectively [26 ]. Briefly, cells were seeded in the upper Boyden chambers of the cell culture inserts. After 24 h, the cells that had adhered to the lower membrane were stained with DAPI in the dark and then visualized and counted. Images of three random fields were captured at 200× magnification under a microscope. The number of cells on the bottom surface in each group was compared.
7
Transwell Migration and Invasion Assay
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Transwell assays were performed to assess cell migration and invasion using BD BioCoat Control Cell Culture Inserts or a BD BioCoat BD Matrigel Invasion Chamber, respectively. In brief, cells were seeded in the upper Boyden chambers of the cell culture inserts. After incubating for 24 h (for migration) or 36 h (for invasion), cells remaining in the upper chamber (for migration) or on the upper membrane (for invasion) were carefully removed. Cells adhering to the lower membrane were stained with DAPI in the dark, imaged, and counted using an inverted microscope equipped with a digital camera. Five random fields were captured at 200× magnification under microscope. The number of cells on the bottom surface was compared between groups.
8
Cell Migration and Invasion Assay
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Cell migration and invasion assays were performed using BD BioCoat control cell culture inserts (BD Biosciences, USA) and Matrigel invasion chambers (BD Biosciences, USA), respectively. In brief, cells (5 × 104 cells/well) suspended in FBS-free DMEM/F12 culture medium were added into the upper chamber, and 1 mL of DMEM/F12 containing 10% FBS was added to the lower compartment. After incubation for 24 h, the cells that remained on the upper surface of the filter membrane were wiped off, and the cells that had crossed the filter membrane and adhered on the lower membrane surface were stained with 0.1% crystal violet. Images of the stained lower surface were captured, and the cells were quantified under a microscope in five random fields.
9
Transwell Assays for Cell Migration and Invasion
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Transwell assays were performed to evaluate cell migration and invasion abilities using BD BioCoat Control Cell Culture Inserts or BD BioCoat BD MatrigelTM Invasion Chamber, respectively [15 (link)]. In brief, cells were seeded into the upper Boyden chambers of cell culture inserts. After 24 h of incubation, cells adhering to the lower membrane were stained with DAPI in the dark, imaged and counted using an inverted microscope equipped with a digital camera. Three random fields were captured at 200× magnification.
10
Transwell Assay for Migration and Invasion
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Transwell assays were performed to assess the cell migration and invasion ability using BD BioCoat Control Cell Culture Inserts and BD BioCoat BD Matrigel™ Invasion Chambers, respectively [26 (link)]. Briefly, cells were seeded in the upper Boyden chambers of the cell culture inserts. After 24h of incubation, cells that adhered to the lower membrane were stained with DAPI in the dark, imaged and counted. Three random fields were captured at 200× magnification under a microscope. The number of cells on the lower surface was compared among the groups.
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