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96 well shuttle device

Manufactured by Lonza
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The 96-well Shuttle Device is a laboratory equipment designed to efficiently transfer and transport multi-well plates between different workstations. It provides a controlled and consistent handling process for 96-well plates, ensuring reliable sample transfer within a laboratory environment.

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Lab products found in correlation

Xfect transfection reagent, by Takara Bio (2 mentions) Cell line 96 well nucleofector kit sf, by Lonza (2 mentions) P3 primary cell nucleofector solution, by Lonza (1 mentions)

Spelling variants of this product

96-well Shuttle Nucleofector device (4 citations)

3 protocols using 96 well shuttle device

1

Transfection of siRNA and Expression Vectors

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For transfection of small interfering RNA (siRNA), 200 pmol siRNA duplex was transfected using the Cell Line 96‐well Nucleofector Kit SF (Lonza) and program DG150 of the 96‐well Shuttle Device (Lonza). For transfection of expression vectors, expression vectors were transfected using Xfect Transfection Reagent (TaKaRa Clontech), in accordance with the manufacturer's instructions. All siRNA sequences used in this study are listed in Supplementary Table S1.
Takeuchi C., Yokoshi M., Kondo S., Shibuya A., Saito K., Fukaya T., Siomi H, & Iwasaki Y.W. (2022). Mod(mdg4) variants repress telomeric retrotransposon HeT-A by blocking subtelomeric enhancers. Nucleic Acids Research, 50(20), 11580-11599.
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2

Efficient mESC Transfection Protocol

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mESCs were trypsinized and washed with 1× PBS (Nacalai Tesque) once. Cells (2 × 105) were transfected with 40 pmol of siRNA and 20 μl of the P3 Primary Cell Nucleofector Solution (Lonza; supplement 1 added) using program CG-104 in 96-well Shuttle Device (Lonza) according to the manufacturers’ instructions. Transfected cells were then seeded into iMatrix-511 silk–precoated culture dish for culture and further experiments.
Li T.D., Murano K., Kitano T., Guo Y., Negishi L, & Siomi H. (2022). TDP-43 safeguards the embryo genome from L1 retrotransposition. Science Advances, 8(47), eabq3806.
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3

Efficient Transfection of siRNA and Expression Vectors

Check if the same lab product or an alternative is used in the 5 most similar protocols
For transfection of small interfering (si)RNA, 200 pmol siRNA duplex was transfected using the Cell Line 96-well Nucleofector Kit SF (Lonza) and program DG150 of the 96-well Shuttle Device (Lonza). For transfection of expression vectors, expression vectors were transfected using Xfect Transfection Reagent (TaKaRa Clontech), in accordance with the manufacturer's instructions. All siRNA sequences used in this study are listed in Supplementary Table 1.
Takeuchi C., Yokoshi M., Kondo S., Shibuya A., Saito K., Fukaya T., Siomi H., & Watanabe Y. (2022). Mod(mdg4) variants repress telomeric retrotransposon HeT-A by blocking subtelomeric enhancers.
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