Clone sx53g8

Manufactured by Agilent Technologies

The Clone SX53G8 is a laboratory instrument designed for molecular biology applications. It serves as a versatile tool for amplifying and analyzing DNA samples. The device utilizes polymerase chain reaction (PCR) technology to selectively replicate targeted DNA sequences. Its core function is to facilitate the accurate and efficient duplication of genetic material for further analysis and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

Mayer s hematoxylin, by Leica (1 mentions) Bond max, by Leica (1 mentions) Bond 3, by Leica (1 mentions) Anti p53 clone do7, by Agilent Technologies (1 mentions) Envision kit, by Agilent Technologies (1 mentions)

2 protocols using clone sx53g8

Immunohistochemical Protein Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

IHC was performed using the Bond Max and Bond III autostainers (Leica Microsystems, Wezlar, Germany). For p21 protein expression, the mouse monoclonal antibody (Clone SX118, Dako, DK; 1:60 dilution, 30' incubation) was used. Proteins p27 and p63 were assessed using mouse monoclonal antibodies (clone SX53G8, Dako; at dilution 1:150, 30' incubation and clone 7JUL at 1:50 dilution, 1h incubation at room temperature respectively). p53 protein expression was assessed with anti-p53 clone DO7 (DAKO) at 1:100 dilution, upon antigen retrieval in citric acid for 20 min. Cyclin D1 protein expression was assessed using the rabbit monoclonal antibody (clone SP4, Spring Bioscience, USA; 1:200 dilution, 1h incubation at room temperature), cyclin E1 protein using the rabbit monoclonal antibody (clone EP435E, Novus Biologicals, Littleton, CO; 1:80 dilution, 30' incubation) and CD117 with the polyclonal rabbit antibody (code A4502, Dako). The antigen–antibody complex was visualized using DAB as a chromogen. Slides were counterstained with Mayer’s hematoxylin for 10 min (Leica), washed in water, dehydrated and mounted.

Zagouri F., Kotoula V., Kouvatseas G., Sotiropoulou M., Koletsa T., Gavressea T., Valavanis C., Trihia H., Bobos M., Lazaridis G., Koutras A., Pentheroudakis G., Skarlos P., Bafaloukos D., Arnogiannaki N., Chrisafi S., Christodoulou C., Papakostas P., Aravantinos G., Kosmidis P., Karanikiotis C., Zografos G., Papadimitriou C, & Fountzilas G. (2017). Protein expression patterns of cell cycle regulators in operable breast cancer. PLoS ONE, 12(8), e0180489.

+ Open protocol

+ Expand

Quantifying p27 Expression in Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed using a monoclonal antibody against p27 (clone SX53G8; Dako, Carpinteria, CA). Staining was performed on 4-μm tissue sections cut from formalin-fixed, paraffin-embedded tissues. Each section was deparaffinized, rehydrated, and subjected to heat-induced epitope retrieval in citrate buffer (pH 6). Endogenous peroxidase activity was blocked with 3% hydrogen peroxide. The sections were incubated with the primary antibody at a dilution of 1:500 for 1 hour at room temperature, and detected with the Dako Envision Kit. Sections from normal pancreas served as the positive control. Citrate buffer was substituted for the primary antibody in the negative control.
Each sample was examined by a gastrointestinal pathologist and assigned an H-score to quantify the level of expression of p27. The H-score is calculated as the product of the extent (% of cells) and intensity (0, none; 1+ faint; 2+ moderate; 3+ strong) of staining. If multiple sections of the same tumor were stained, the average H-score was reported. The nuclear and cytoplasmic expression of p27 was determined for every sample.

Maxwell J.E., Sherman S.K., Li G., Choi A.B., Bellizzi A.M., O’Dorisio T.M, & Howe J.R. (2015). Somatic alterations of CDKN1B are associated with small bowel neuroendocrine tumors. Cancer genetics.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!