3 aminopropyl triethoxysilane aptes

Manufactured by Tokyo Chemical Industry

Sourced in United States

(3-Aminopropyl) triethoxysilane (APTES) is a silane coupling agent with the chemical formula C9H23NO3Si. It is a clear, colorless liquid used as a precursor in various applications, including surface modification, adhesion promotion, and chemical functionalization.

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5 protocols using 3 aminopropyl triethoxysilane aptes

Functionalized Nanoparticle Synthesis and Conjugation

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All chemicals
and reagents used were of analytical grade. Poly-l-lysine
(PLL) hydrochloride (M.W. >12 000, cutoff by dialysis) was
purchased from PEPTIDE INSTITUTE. PEGylated, long-chain SPDP cross-linker
(PEG4-SPDP) and Alexa Fluor 430 NHS ester were obtained from Thermo
Fisher Scientific. Citrate-coated colloidal silver nanoparticles (AgNPs)
with a mean diameter of 80 nm were purchased from NanoComposix. Amino-functionalized
polystyrene (PS) nanospheres with a mean diameter of 100 nm were obtained
from Polysciences. Bissulfosuccinimidyl suberate (BS3) was supplied
by Dojindo. Dimethyl sulfoxide (DMSO) was purchased from Fujifilm
Wako Pure Chemical Corp. Tris(2,2′-bipyridyl)ruthenium(II)
chloride hexahydrate, cysteamine, and (3-aminopropyl)triethoxysilane
(APTES) were purchased from Tokyo Chemical Industry Co., Ltd (TCI).

Kato R., Uesugi M., Komatsu Y., Okamoto F., Tanaka T., Kitawaki F, & Yano T.A. (2022). Highly Stable Polymer Coating on Silver Nanoparticles for Efficient Plasmonic Enhancement of Fluorescence. ACS Omega, 7(5), 4286-4292.

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Synthesis and Characterization of Multifunctional Nanoparticles

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Tetraethyl orthosilicate (TEOS), 1H-imidazole-4-carboxylic acid, thionyl chloride (SOCl₂), Traut’s reagent (2-iminothiolane), Triton X-100, acetone, ethanol, glutathione (GSH), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and ammonia (30% in water) were purchased from Fisher Scientific, USA. Hexanol, cyclohexane, and (3-aminopropyl)triethoxysilane (APTES) were bought from Tokyo Chemical Industry Co., Ltd., USA. Triethylamine (TEA) and dimethyl sulfoxide (DMSO) were purchased from Alfa Aesar, USA. Bis[3-(triethoxysilyl)propyl]-disulfide (BTPD) was purchased from Gelest, Inc., USA. Methoxy-poly(ethylene glycol)-silane (mPEG-silane, M_n = 5000), amine-poly(ethylene glycol)-silane (NH₂-PEG-silane, M_n = 5000) and maleimide-poly(ethylene glycol)-silane (Mal-PEG-silane, M_n = 5000) were purchased from Biochempeg Scientific Inc., USA. All-trans-retinoic acid (ATRA) and 4-aminophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside were purchased from Santa Cruz Biotechnology, USA. Nuclear localization signal (NLS)-tagged Streptococcus pyogenes Cas9 nuclease (sNLS-SpCas9-sNLS) was obtained from Aldevron, USA. In vitro transcribed single guide RNAs (sgRNAs) and ssODNs were purchased from Integrated DNA Technologies, Inc., USA.

Wang Y., Shahi P.K., Wang X., Xie R., Zhao Y., Wu M., Roge S., Pattnaik B.R, & Gong S. (2021). In Vivo Targeted Delivery of Nucleic Acids and CRISPR Genome Editors Enabled by GSH-Responsive Silica Nanoparticles. Journal of controlled release : official journal of the Controlled Release Society, 336, 296-309.

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Bioconjugation of GO with IgG

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GO dispersion was purchased from Nanjing XFNANO Materials Tech Co., Ltd. (Jiangsu, China); (3-Aminopropyl) triethoxysilane (APTES) was purchased from Tokyo Chemical Industry Co., Ltd. (Shanghai, China); 1-(3-Dimethylamino-propyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) were purchased from Saan Chemical Technology Co., Ltd. (Shanghai, China); immunoglobulin G (IgG) from rabbit serum, anti-rabbit IgG antibody produced in goat, rabbit serum, and phosphate buffer solution (PBS, pH 7.4) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China); and bovine serum albumin (BSA), glutaraldehyde, and other chemicals were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China). All of the reagents were of analytical grade and could be used without further purification. All proteins were dissolved into PBS before use.

Chen S., Zhang C., Wang J., Li N., Song Y., Wu H, & Liu Y. (2023). A Fiber Bragg Grating Sensor Based on Cladding Mode Resonance for Label-Free Biosensing. Biosensors, 13(1), 97.

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Multimode Fiber-Optic Biofilm Sensor

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Yeast extract was obtained from Becton, Dickinson and Company (USA). Glucose, crystal violet, acetone, and sodium hydroxide were purchased from Wako Pure Chemical Industries (Japan). A biofilm inhibiter was obtained from Gospel Toshin Ltd. (Japan). Poly(allylaminehydrochloride) (PAH) and poly-{1-[4-((3-carboxy-4-hydroxyphenyl) azo)-benzenesulfonamido]-1,2-ethanediyl, sodium salt} (PCBS) were obtained from Sigma-Aldrich (USA). (3-Aminopropyl) triethoxysilane (APTES) was purchased from Tokyo Chemical Industry (Japan). All chemicals were used as received without further purification, and Milli-Q water was used in solution preparation.
A multimode plastic-clad silica fiber (FT200EMT, refractive index: 1.44) with a 200 μm-step index for the core diameter was purchased from ThorLabs (USA). The cladding was removed 8 cm length from the center using acetone, and the exposed fiber core was used as the sensing region.

Orii T., Okazaki T., Hata N., Sugawara K., Rahman F.A, & Kuramitz H. (2017). Development of an Attenuated Total Reflection Based Fiber-Optic Sensor for Real-time Sensing of Biofilm Formation. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 33(8).

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AuNPs-based Thrombin Aptamer Assay

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AuNPs (40 nm in diameter) were obtained from BBI solutions (Cardiff, UK). Thrombin binding aptamer (Th-apt, 5′-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3′) was purchased from Eurofins Genomics (Tokyo, Japan). 22 2-Melcaptethanol (2-ME) and methyl orange were obtained from Wako (Osaka, Japan). 3-Aminopropyltriethoxysilane (APTES) and bovine serum albumin (BSA) were obtained from Tokyo Chemical Industry (Tokyo, Japan) and Bio RAD (Hercules, CA, USA), respectively. Slide glass and cover glass were purchased from Matsunami Glass Industry (Osaka, Japan).

Yano Y., Nisougi M., Yano-Ozawa Y., Ohguni T., Ogawa A., Maeda M., Asahi T, & Zako T. (2019). Detection of Gold Nanoparticles Aggregation Using Light Scattering for Molecular Sensing. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 35(6).

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