Doramectin

Manufactured by MedChemExpress

Sourced in China

Doramectin is a laboratory equipment product manufactured by MedChemExpress. It is a chemical compound used for research purposes.

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Lab products found in correlation

2 protocols using doramectin

ZIKV Infection Assay in Cell Lines

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The human lung carcinoma cell line A549, African green monkey kidney epithelial cell line Vero, and human astrocytoma cell line SNB19 were obtained from the Cell Bank of the Chinese Academy of Sciences (CBCAS), Shanghai, China and cultured in DMEM (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (GIBCO, Carlsbad, USA), 2 mM L-glutamine, 100 μg/mL streptomycin, and 100 units/mL penicillin (Invitrogen, Carlsbad, USA) at 37 °C under 5% CO₂. All cell lines were authenticated by short tandem repeat (STR) fingerprinting at the Medicine Laboratory of Forensic Medicine Department of Sun Yat-Sen University (SYSU) (Guangzhou, China), and were found to be free of mycoplasma contamination. ZIKV ZG-01 strain (China, 2016, GenBank accession number KY379148), doramectin (catalog no.: HY-17035, batch no.: 109519) and ribavirin (catalog no.: HY-B0434, batch no.: 11970) were supplied in powder form by MedChemExpress LLC (Shanghai, China) with quality control documents.

Zhu Y., Liang M., Yu J., Zhang B., Zhu G., Huang Y., He Z, & Yuan J. (2023). Repurposing of Doramectin as a New Anti-Zika Virus Agent. Viruses, 15(5), 1068.

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Screening Spectrum Collection Compounds for OATP1B1 Inhibition

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Compounds from the Spectrum Collection, distributed in 80 wells of 96 well plates (100 nmol per well in 10 μl of DMSO), were screened for their inhibitory effectiveness against transport of E3S into CHO cells expressing OATP1B1; a total of 560 compounds were used. Each compound was diluted to a concentration of 20 μM, pH 7.4, to a final concentration of 2% dimethylsulfoxide (DMSO) using a VIAFLO multichannel electronic pipet (Integra Biosciences, Hudson, NH) ⁶⁵. The following compounds for prospective testing were purchased from MedChemExpress (Monmouth Junction, NJ): abamectin, asiaticoside, baloxavir, berbamine, bremelanotide, bromocriptine, cabazitaxel, doramectin, etoposide, lapatinib, mobocertinib, novobiocin, posaconazole, rifaximin, teniposide, umbralisib, vancomycin, vinblastine and vincristine. Pyronaridine tetraphosphate was purchased from BOC Sciences (Shirley, NY) and tilorone hydrochloride from Caymen Chemical Company (Ann Arbor, MI). These compounds were solubilized in DMSO at 20 mM prior to tested.

Lane T.R., Urbina F., Zhang X., Fye M., Gerlach J., Wright S.H, & Ekins S. (2022). Machine Learning Models Identify New Inhibitors for Human OATP1B1. Molecular pharmaceutics, 19(11), 4320-4332.

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