The amount of lactate in dissected guts (n = 20, same number of males and females) was determined using a Lactate Assay Kit (Sangon Biotech, Shanghai, China) following the manufacturer’s instructions and was normalized to the total protein content.
Lactate assay kit
Manufactured by Sangon
Sourced in China
Lactate assay kit is a laboratory equipment used to quantitatively measure lactate levels in various biological samples. It provides a reliable and efficient method for analyzing lactate concentration.
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Lab products found in correlation
4 protocols using lactate assay kit
1
Gut Lactate Quantification Protocol
2
Metabolic Analysis of ESCC Cells
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ESCC cells (1 × 106) were seeded into a 6-well plate and cultured for 24 h until cells adhered. ATP and pyruvate levels were assessed as per the ATP Assay kit and Pyruvate Assay kit instructions (Sangon Biotech, Shangahi China). To measure glucose and lactate concentrations, the culture medium was swapped with phenol-red free DMEM containing 1% FBS for another 24 h, after which the medium was collected. Glucose uptake was evaluated using a kit (Abcam, USA), while the consumed glucose level was determined by subtracting the detected glucose concentration in the medium from the initial one. The lactate production assessment followed the Lactate Assay kit instructions (Sangon Biotech, Shanghai, China). The obtained results were adjusted based on cell numbers to ensure accuracy.
3
Quantifying Cellular Lactate Production
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The lactate production of cells was measured using a lactate assay kit (Sangon Biotech, Shanghai, China). Cells were seeded in the 6-well plate in triplicates. Supernatants were collected after 24 h incubation. The lactate level was normalized to the cell numbers.
4
Lactic Acid Production Assay Protocol
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All procedures of lactic acid production assay were repeated as illustrated previously [25 (link)]. The concentration of lactic acid in the harvested lysates was determined using a lactate assay kit (D799851, Sangon Biotech, Shanghai, China) in accordance with the protocols of the manufacturer. MCF-7 and MDA-MB-231 cells (5 × 105 cells/well) with or without transfection were cultured in six-well plates and resuspended in extraction buffer I, followed by lysis at a power of 300 W for 3 min in total (3 s of ultrasonication, at an interval of 7 s) using an XC-II D ultrasonic cell crusher (Nanjing Ningkai Instrument Co., Ltd, Nanjing, China). The lysates were harvested after centrifugation at 12,000 × g at 4°C for 10 min in a 5810 Centrifuge (Eppendorf, Hamburg, Germany). A volume of 0.8 mL of the supernatant was collected and added with 0.15 mL of extraction buffer II, followed by another centrifugation at 12,000 × g at 4°C for 10 min. The absorbance at 570 nm was monitored by Varioskan™ LUX multimode microplate reader (VLBLATD2; ThermoFisher Scientific, Waltham, MA, USA).
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