Telomerase activity was assessed by the TRAP assay (TRAPeze kit, Millipore, Temecula, CA, USA) according to the manufacturer’s instructions in samples which were exposed to the IC50 dose of the drugs. Cells were lysed with ice-cold CHAPS lysis buffer, 50–100 ng of protein extracts were subjected to PCR in the presence of TS primer. The PCR products were separated on 12.5% PAGE, stained with Nucleic Acid Gel Stain (Lonza, Basel, Switzerland) and quantified by the Quantity One software in the Versa-Doc device. TA was calculated according to the following formula: TPG = [(X−B)/C]:[(r−B)/Cr × 100], where TPG is the total product generated, X signifies each sample signal, B is the gel background, C represents the 36 bp internal PCR control, r is the TSR8 quantification control.
Nucleic acid gel stain
Manufactured by Lonza
Sourced in Switzerland
Nucleic Acid Gel Stain is a laboratory product used to visualize and detect nucleic acids, such as DNA and RNA, in agarose or polyacrylamide gels after electrophoresis. The stain binds to the nucleic acids and emits fluorescence when exposed to ultraviolet (UV) light, allowing for the identification and analysis of the separated DNA or RNA fragments.
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Lab products found in correlation
2 protocols using nucleic acid gel stain
1
Telomerase Activity Quantification by TRAP
2
Quantification of Telomerase Activity
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Telomerase activity was assessed by the TRAP assay (TRAPeze kit, Millipore) according to the manufacturer’s instructions in samples which were exposed to the IC50 dose of bortezomib. Cells were lysed with ice cold CHAPS lysis buffer, 50-100 ng of protein extracts were subjected to PCR in the presence of TS primer. The PCR products were separated on 12.5% PAGE and were stained with Nucleic Acid Gel Stain (Lonza, Basel Switzerland). Quantification was performed by the Quantity One software in the Versa-Doc device. TA was calculated according to the following formula: TPG = [(X − B)/C]:[(r − B)/Cr⁎100], where TPG is the total product generated, X signifies each sample signal, B is the gel background, C represents the 36 bp internal PCR control, r is the TSR8 quantification control.
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