Alexa fluor 488 555 donkeyanti mouse

Manufactured by Thermo Fisher Scientific

The Alexa Fluor 488/555 donkey anti-mouse is a fluorescently labeled secondary antibody designed for use in immunofluorescence and other fluorescence-based applications. The Alexa Fluor 488 and 555 dyes are conjugated to a donkey anti-mouse antibody, providing a specific detection tool for mouse primary antibodies.

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Lab products found in correlation

Goat anti sox17 antibody, by R&D Systems (2 mentions) Hoechst, by Merck Group (2 mentions) Goat anti oct4 antibody, by Santa Cruz Biotechnology (2 mentions) Rabbitanti foxa2 antibody, by Merck Group (2 mentions) Goatanti foxa2 antibody, by R&D Systems (2 mentions) Mouse anti aat antibody, by Thermo Fisher Scientific (2 mentions) Goat anti pdx1 antibody, by R&D Systems (2 mentions) Rabbit anti nanog antibody, by Abcam (2 mentions) Mouse anti afp antibody, by Merck Group (2 mentions) Mouse anti ck18 antibody, by Abcam (2 mentions) Goat anti albumin antibody, by Fortis Life Sciences (2 mentions) Anti rabbit or anti goat igg, by Thermo Fisher Scientific (2 mentions) Eclipse te2000 u microscope, by Nikon (2 mentions)

Close spelling variants of this product

Alexa Fluor 488/555 Alexa Fluor 555 Alexa Fluor 488 Alexa 555 Alexa 488 Alexa Fluors

2 protocols using alexa fluor 488 555 donkeyanti mouse

Immunostaining Protocol for Lineage Markers

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Standard immunostaining was carried out as previously reported^{13 (link)}. Primary antibodies were goat
anti-SOX17 antibody (R&D, Cat # AF1924) diluted 1: 1,000, rabbit
anti-FOXA2 antibody (Millipore, Cat # AB4125) diluted 1:500, goat
anti-FOXA2 antibody (R&D, Cat # AF2400) diluted 1:1,000, mouse
anti-HNF4α antibody (Perseus Proteomics, Cat # PP-H1415-00)
diluted 1:500, mouse anti-AAT antibody (Thermo Scientific, Cat #
MA190438) diluted 1:500, goat anti-PDX1 antibody (R&D, Cat #
AF2419) diluted 1:1,000, goat anti-OCT4 antibody (Santa Cruz Biotechnology, Cat
# sc-8629) diluted 1:500, rabbit anti-NANOG antibody (Abcam, Cat
# ab80892) diluted 1:500, mouse anti-AFP antibody (Sigma-Aldrich, Cat
# A8452) diluted 1:500, mouse anti-CK18 antibody (Abcam, Cat #
ab82254) diluted 1:500, and goat anti-albumin antibody (Bethyl, Cat #
A80-129A) diluted 1:500. Secondary antibodies were Alexa Fluor 488/555 donkey
anti-mouse or anti-rabbit or anti-goat IgG (1:1,000) (Invitrogen). Nuclei were
visualized by Hoechst (Sigma-Aldrich) staining. Images were captured using a
Nikon Eclipse TE2000-U microscope.

Zhu S., Rezvani M., Harbell J., Mattis A.N., Wolfe A.R., Benet L.Z., Willenbring H, & Ding S. (2014). Mouse liver repopulation with hepatocytes generated from human fibroblasts. Nature, 508(7494), 93-97.

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Immunostaining Protocol for Lineage Markers

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