Monkey ifabp fabp2 and lbp elisa kits

Plasma samples were thawed and cleared using Ultrafree centrifugal filters (Millipore, Billerica, MA, USA). Cytokines, chemokines, and growth factors were quantified in plasma using the Non-Human Primate Cytokine/Chemokine/Growth Factor 37-Plex ProcartaPlex Panel (Invitrogen, Life Technologies, Waltham, MA, USA), following the manufacturer’s instructions. Data were acquired with a Bio-Plex 200 analyzer and analyzed using Bio-Plex Manager software v6.1 (Bio-Rad, Hercules, CA, USA). Commercially available Monkey IFABP/FABP2 and LBP ELISA kits (MyBioSource, San Diego, CA, USA) were used to quantify the intestinal fatty acid binding protein (IFABP) and LPS-binding protein (LBP) in plasma samples. The commercially available ELISA kit for Human sCD14 (R&D Systems, Minneapolis, MN, USA) was used according to the manufacturer’s protocols with 1:200 dilution of plasma samples. All assays were performed in duplicate, and data were analyzed using Gen 5 software (BioTek, Winooski, VT, USA).

Frozen plasma samples were thawed and cleared using Ultrafree Centrifugal Filters (Millipore, Billerica, MA). The filtered plasma samples were used for simultaneous quantification of cytokines, chemokines, and growth factors using the multiplexed-bead assay Non-Human Primate Cytokine & Chemokine & Growth Factor 37-plex ProcartaPlex (Invitrogen, Life Technologies), following manufacturers’ instructions. Data were acquired with a Bio- Plex 200 analyzer (Bio-Rad, Hercules, CA) and analyzed using Bio-Plex Manager software v6.1 (BioRad). For the analysis of markers of leaky gut and microbial translocation, plasma IFABP and LBP were quantified using the commercially available Monkey IFABP/FABP2 and LBP ELISA kits (MyBioSource, San Diego, CA). Commercially available ELISA kit for Human sCD14 (R&D Systems, Minneapolis, MN) was used according to the manufacturer’s protocols with 1:200 diluted plasma samples. All tests were performed according to the manufacturer’s guidelines. The assays were performed in duplicate, and data were analyzed using Gen 5 software (BioTek).