Uveitis was induced by intracameral injection of 100 ng of E. coli LPS (Sigma #L4391, St. Louis, MO) in 20 μl PBS (Mediatech, Manassas, VA) into the right eye using a 29 gauge needle, while the rabbits were anesthetized with ketamine HCl (25 mg/kg, Ft. Dodge) and xylazine (2 mg/kg, Lloyd) IM. Topical proparacaine (0.5%, Akorn, Lake Forest, IL) was applied to the ocular surface prior to intraocular injections. The ocular surface was prepared for the injection procedure using a dilute solution of 5% Povidone iodine (Aurora Pharmaceutical, Northfield, MN) in 0.9% saline (Phoenix, Burlingame, CA). During anesthesia (preparation and recovery) the cornea was protected from drying by irrigation with Balanced Salt Solution (Akorn) or, following intraocular injection, the application of ocular lubricant (Refresh Tears, Allergan) or Bacitracin-Polymixin B Preservative Free Ophthalmic Ointment (Akorn). The left eye received all the pre- and post- injection treatments, but not the LPS or PBS injection and thus was used as a procedural control for ocular preparation.
Bacitracin polymixin b preservative free ophthalmic ointment
Manufactured by Akorn
Bacitracin-Polymixin B Preservative Free Ophthalmic Ointment is a sterile, preservative-free ophthalmic ointment that contains the active ingredients bacitracin and polymixin B. It is intended for topical application to the eye.
Automatically generated - may contain errors
2 protocols using bacitracin polymixin b preservative free ophthalmic ointment
1
Induction of Uveitis in Rabbits
2
Induction of Uveitis in Rabbits
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Uveitis was induced by intracameral injection of 100 ng of E. coli LPS (Sigma #L4391, St. Louis, MO) in 20 μl PBS (Mediatech, Manassas, VA) into the right eye using a 29 gauge needle, while the rabbits were anesthetized with ketamine HCl (25 mg/kg, Ft. Dodge) and xylazine (2 mg/kg, Lloyd) IM. Topical proparacaine (0.5%, Akorn, Lake Forest, IL) was applied to the ocular surface prior to intraocular injections. The ocular surface was prepared for the injection procedure using a dilute solution of 5% Povidone iodine (Aurora Pharmaceutical, Northfield, MN) in 0.9% saline (Phoenix, Burlingame, CA). During anesthesia (preparation and recovery) the cornea was protected from drying by irrigation with Balanced Salt Solution (Akorn) or, following intraocular injection, the application of ocular lubricant (Refresh Tears, Allergan) or Bacitracin-Polymixin B Preservative Free Ophthalmic Ointment (Akorn). The left eye received all the pre- and post- injection treatments, but not the LPS or PBS injection and thus was used as a procedural control for ocular preparation.
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