1 μg of bacterial RNA was reverse transcribed to generate cDNA using random hexamers and the Superscript III Reverse Transcriptase Synthesis System (Invitrogen 18080051) according to the manufacturer’s recommendations. qPCR was performed with primers annealing to internal regions of the target genes (Supplementary Table S1) using SYBR™ Green PCR Master Mix (Applied Biosystems 4309155) on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Data was obtained through QuantStudio Real-Time PCR Software v1.3. Relative mRNA abundances were
![]()
R a t i o = E f f c i e n c y ( t a r g e t ) C T ( t a r g e t , u n t r e a t e d ) − C T ( t a r g e t , t r e a t e d ) E f f c i e n c y ( r e f ) C T ( r e f , u n t r e a t e d ) − C T ( r e f , t r e a t e d )
Superscript 3 reverse transcriptase synthesis system
Manufactured by Thermo Fisher Scientific
Sourced in Canada
The SuperScript III reverse transcriptase synthesis system is a laboratory instrument designed for the enzymatic conversion of RNA into complementary DNA (cDNA). It provides a reliable and efficient method for the reverse transcription of RNA into single-stranded cDNA, which can then be used for various downstream applications, such as gene expression analysis, RT-PCR, and cDNA library construction.
Automatically generated - may contain errors
Lab products found in correlation
Taqman gene expression assay, by Thermo Fisher Scientific (2 mentions)
Taqman universal pcr master mix, by Thermo Fisher Scientific (2 mentions)
Abi prism 7900 sequence detection system, by Thermo Fisher Scientific (2 mentions)
Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Quantstudio 6 flex real time pcr system, by Thermo Fisher Scientific (1 mentions)
Tri reagent, by Merck Group (1 mentions)
Rneasy kit, by Qiagen (1 mentions)
Tri reagent, by Molecular Research Center (1 mentions)
3 protocols using superscript 3 reverse transcriptase synthesis system
1
Quantifying Bacterial mRNA Expression via qPCR
2
Cardiac mRNA Quantification by RT-qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA was isolated from cardiac extracts using TRI reagent (Sigma). First-strand cDNA was synthesized from total RNA using the SuperScript III reverse transcriptase synthesis system (Invitrogen). Real-time polymerase chain reaction was performed with the ABI Prism 7900 Sequence Detection System using TaqMan Gene Expression Assays and TaqMan Universal PCR Master Mix (Applied Biosystems). Relative levels of mRNA transcripts were quantified using the 2-ΔCt method and normalized to levels of peptidyl-prolyl isomerase A (Ppia-cyclophilin) RNA.
3
First-Strand cDNA Synthesis and qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
First-strand complementary DNA was synthesized according to the manufacturer’s protocol (RNeasy Kit from Qiagen, Mississauga, ON, Canada; or Tri-Reagent from Molecular Research Center, Inc., Cincinnati, OH) using the SuperScript III reverse transcriptase synthesis system (Invitrogen, Carlsbad, CA) and random hexamers. Quantitative PCR was performed with the ABI Prism 7900 Sequence Detection System using the TaqMan Gene Expression Assays and TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!