Myocardial tissues were grounded and homogenized with lysis solution. After sonication, the lysates were centrifuged, and the proteins were separated using electrophoresis and transformation to polyvinylidene fluoride (PVDF) membranes. After being blocked with 5% skim milk in Tris-buffered saline (TBS) for 2 hours at room temperature, the membrane was incubated with primary antibodies against BDNF rabbit antibody (1/1000 dilution; Abcam, Cambridge, UK), Bax, Bcl-2, caspase-3, cleaved caspase-3 rabbit antibody (1/1000 dilution; Cell signaling Tecnology, USA), and GAPDH mouse antibody (1/1000 dilution; Cell signaling Tecnology, USA) overnight at 4°C, washed three times with TBST, and then incubated with horseradish peroxidase-conjugated secondary antibody for 1 hour at 37°C. The blots were imaged using AlphaView system (Cell Biosciences, Santa Clara, CA, USA) and quantified using the Image J 1.48 software (National Institutes of Health).
Bdnf rabbit antibody
Manufactured by Abcam
Sourced in United Kingdom, United States
The BDNF (Brain-Derived Neurotrophic Factor) rabbit antibody is a tool used in research applications. It specifically recognizes the BDNF protein, which is a member of the neurotrophin family and plays a crucial role in the growth, development, and survival of neurons.
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Lab products found in correlation
2 protocols using bdnf rabbit antibody
1
Myocardial Protein Analysis via Western Blot
2
Immunohistochemical Analysis of Brain Markers
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Mice were deeply anesthetized and perfused with 4% paraformaldehyde. Brain sections (25 µm) were blocked with 3% donkey serum for 2 hours at room temperature prior to incubation with one of the following primary antibodies overnight at 4°C: BDNF rabbit antibody (1:200; Abcam, USA), glial fibrillar acidic protein (GFAP) mouse antibody (1:500; Cell Signaling Technology, USA), the neuronal nuclear protein (NeuN) mouse antibody (1:100; Cell Signaling Technology), and ionized calcium-binding adapter molecule-1 (IBA-1) goat antibody (1:500; Novusbio, USA). After washing with PBS, sections were incubated with an appropriate secondary antibody for 2 hours. The sections were mounted and observed under a Leica SP8 laser confocal microscope.
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