Adherent cells were seeded in 6-well plates and SC in ULA 6-well plates in their respective medium. After 5 days, cells were dissociated into single-cell suspension, stained with Trypan blue and counted with a cell counting device (CEDEX, Roche).
Cedex
Manufactured by Roche
Sourced in Germany
The Cedex is a laboratory instrument manufactured by Roche. It is designed to perform automated cell counting and analysis. The Cedex provides accurate and reliable measurement of various cell parameters, including cell concentration, cell viability, and cell size distribution. The core function of the Cedex is to assist in cell culture monitoring and optimization, supporting researchers and scientists in their work.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using cedex
1
Cell Counting Protocol for Adherent and Suspension Cells
2
Homogeneous Cell Concentration Maintenance
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To print homogeneous cell concentrations for >1 h, a cell-mixing system
was developed together with regenHU Ltd. (Switzerland) to avoid cell
sedimentation in the printing cartridge. This stirring system, developed in
the project frame, is now commercially available at regenHU Ltd. The stirrer
was 3D printed using PA2200 material, a commonly used polyamide, with five
propeller triplets (Fig. 2C D
ethanol resistant, which is important for sterilization and reuse. The
modular stirring system fits into a 3-mL printing cartridge (3-cc cartridge;
regenHU Ltd). Stirring speed is continuously adjustable between 0 and 240
rpm. To analyze stirring effect on cell concentration and viability, cells
were printed/jetted through the jetting valve, harvested at different time
points, and analyzed for viability and cell concentration with the cell
counting device CEDEX (Roche, Innovatis, Basel, Switzerland). Samples of
printed/jetted cells through the jetting valve but without stirring system
served as a control.
was developed together with regenHU Ltd. (Switzerland) to avoid cell
sedimentation in the printing cartridge. This stirring system, developed in
the project frame, is now commercially available at regenHU Ltd. The stirrer
was 3D printed using PA2200 material, a commonly used polyamide, with five
propeller triplets (
ethanol resistant, which is important for sterilization and reuse. The
modular stirring system fits into a 3-mL printing cartridge (3-cc cartridge;
regenHU Ltd). Stirring speed is continuously adjustable between 0 and 240
rpm. To analyze stirring effect on cell concentration and viability, cells
were printed/jetted through the jetting valve, harvested at different time
points, and analyzed for viability and cell concentration with the cell
counting device CEDEX (Roche, Innovatis, Basel, Switzerland). Samples of
printed/jetted cells through the jetting valve but without stirring system
served as a control.
3
Ceranib-2 Cytotoxicity Assay in Colon Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following detachment with 0.25% trypsin+EDTA (Sigma), cultured cells were centrifuged at 1200 rpm and 4 °C for 5 minutes and counted with a CEDEX (Roche; Mannheim, Germany) cell counter, seeded overnight in 96 well plates (approximately 10 4 cells per 0.25 mL). The medium was then removed and replaced with either 250 μL of untreated medium (the control) or with ceranib-2 (1 to 50 μM) for 24 or 48 h. The effects of ceranib-2 on colon cancer cell survival were determined by 3-3-(4,5-D-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test (Mossmann 1983). The optical density read at 550 nm with a microplate reader (BioTek; Winooski, VT) from the treated wells was converted to a percentage of living cells against the control using the following formula:
Absorbance of treated cells in each well x 100 / the mean absorbance of control cells.
Absorbance of treated cells in each well x 100 / the mean absorbance of control cells.
4
Cell Culture and Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T98, LnCaP and 3T3 cells were obtained from the American Type Culture Collection (ATCC, USA) and grown in complete medium containing DMEM supplemented with 10 % fetal calf serum (FCS, Sigma) and 1 % penicillin (10000 unit)-streptomycine (10mg/ml) solution (Sigma) in a humidifi ed atmosphere of 95 % O 2 and 5 % CO 2 in air at 37 °C. After confl uence achieved more than 95 %, the cells were detached with 0.25 % trypsin-EDTA (Sigma), centrifuged at 1200 rpm, 4 °C for 5 min and counted with a cell counter (CEDEX, Roche). The cells were then transferred to microplates for cell survival studies and to fl asks for apoptosis and caspase measurements.
5
Cell Growth Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For assaying the cell growth, equal numbers of cells (20,000-30,000) were seeded in 6-well plates in triplicates and treated with SP or αCGRP each day. The cells of one well were detached each day and counted with a cell counter (Cedex, Roche, Germany) for up to 6 days. The doubling time was calculated using the following formula:
6
Cell culture passaging using Accutase
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell cultures were washed twice with room temperature phosphate buffer solution (PBS) (Lonza, Cologne, Germany). Accutase (Sigma Aldrich, Gillingham, UK) was prewarmed and added to each culture vessel before incubating at 37°C for 7 − 10 min. Accutase was quenched by added fresh mTeSR‐1 (Stem Cell Technologies, Vancouver) and counts were conducted in triplicate using Cedex (Roche Innovatis).
7
Comprehensive Blood Biochemistry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting blood samples were collected from the antecubital vein and used for analyses of biochemical indices in the biochemistry laboratory of the hospital. Total cholesterol (TC) and triglyceride (TG) concentrations were determined using an enzymatic colorimetric test. The selective inhibition method and homogeneous enzymatic colorimetric test (Advia1650 Autoanalyzer, Byer Diagnostics, Leverkusen, Germany) were used to measure the levels of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), respectively. Alanine transaminase (ALT), aspartate transaminase (AST) and γ-glutamyl transpeptidase (GGT) were determined with an automated analyzer (Technicon Sequential Multiple Analyzer, Technicon Instruments Corporation, Tarrytown, NY). Fasting blood glucose (FBG) was measured in venous blood. RBC count, white blood cell count, platelet count, and hemoglobin levels were measured using an automatic hematology analyzer (Roche, Cedex, Gemany).
8
Metabolic Profiling of IgLON5 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IgLON5wt and IgLON5kd cells were cultured in growth medium for 1 or 3 days, respectively. Cultured media were collected and an enzyme colorimetric method was performed to estimate glucose, lactate, and NH3 concentrations using a bioanalyzer (Cedex; Roche Diagnostics, Indianapolis, IN, USA).
9
Cell Growth Assay and Doubling Time Calculation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For assaying the cell growth, equal numbers of cells were seeded in 6-well plates. The cells of one well were detached every day and counted with a cell counter (Cedex, Roche, Germany) for up to 6 days. The doubling time was calculated using the following formula:
10
Automated Process Monitoring and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Online process data, i.e. pH and CO2, were automatically logged using a custom process management system. Routine culture samples were drawn daily over the entire process duration. Additional sampling was performed for the iDoE setups from day 0 to 4. Viability, TCD, and VCD were measured using an automated cell counter (Cedex, Roche, Basel, Switzerland), based on a white/dark image classification following trypan blue exclusion staining. Metabolites (e.g. glucose and lactate) were measured in cell free samples, using photometric assays combined in an automated wet chemical analyzer (Konelelab Prime 60i, Thermo Fisher Scientific, Waltham, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!