Egr 1

Manufactured by Merck Group

EGR-1 is a laboratory equipment product manufactured by Merck Group. It is designed for the detection and analysis of the Egr-1 protein, which is a transcription factor involved in various cellular processes. EGR-1 provides a reliable and efficient tool for researchers working in the field of cell biology, gene expression, and signal transduction.

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Lab products found in correlation

2 protocols using egr 1

Chrysin and Sodium Selenite Cytotoxicity Study

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Chrysin (97% pure) was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO), sodium selenite (Na₂SeO₃) was obtained from LOBA Chemie (Mumbai, India), and Dulbecco’s modified Eagle’s medium (DMEM) and the antibiotics streptomycin and penicillin were obtained from HiMedia (Mumbai, India). Antibodies for m-calpain, Lp82, EGR-1, COX-1, caspase-3, caspase-8, caspase-9, and β-actin were purchased from Sigma-Aldrich Chemical Co. and Cell Signaling Technologies (CST; Danvers, MA). Anti-rabbit immunoglobulin (IgG) secondary antibody was obtained from Genei (Bengaluru, India). All other chemicals and reagents were of analytical grade. Milli-Q water (Millipore, Bengaluru, India) was used throughout the experiments.

Sundararajan M., Thomas P.A., Teresa P.A., Anbukkarasi M, & Geraldine P. (2016). Regulatory effect of chrysin on expression of lenticular calcium transporters, calpains, and apoptotic-cascade components in selenite-induced cataract. Molecular Vision, 22, 401-423.

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Antibody-based Protein Detection Protocol

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Antibodies directed against the following proteins are listed in Supplementary Table 2: SRSF1, SM-α-actin, PCNA, p21, EGR1, GFP, Myc, p53, β-actin, Eif-5, KLF5, Bcl-xL.Bcl-xL Angiotensin II (Ang II), and PDGF-BB were from Sigma. Unless indicated otherwise, all other chemicals were from Sigma-Aldrich.

Xie N., Chen M., Dai R., Zhang Y., Zhao H., Song Z., Zhang L., Li Z., Feng Y., Gao H., Wang L., Zhang T., Xiao R.P., Wu J, & Cao C.M. (2017). SRSF1 promotes vascular smooth muscle cell proliferation through a Δ133p53/EGR1/KLF5 pathway. Nature Communications, 8, 16016.

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