U937 cells were suspended at a final concentration of 1×106 cells/mL in media and plated on hEC layers pre-treated with 50 μg/mL histone for 1 h. The co-cultured cells were treated with cytosine D–arabinofuranoside (Ara-C; Hospira Pty Ltd., Mulgrave, Australia) for 24 h or without Ara-C for 48 h. Adherent and non-adherent U937 cells were collected separately and stained with FITC-conjugated CD45 (BD Biosciences), PE-conjugated CD105 (BD Biosciences), 7-AAD (Beckman Coulter, Fullerton, CA).
Fitc conjugated cd45
FITC-conjugated CD45 is a laboratory reagent used for the identification and enumeration of hematopoietic cells. CD45 is a pan-leukocyte marker expressed on the surface of all cells of hematopoietic origin. The FITC conjugation allows for the detection of CD45-positive cells using flow cytometry.
Lab products found in correlation
4 protocols using fitc conjugated cd45
Adhesion Molecule Expression in hEC-U937 Co-culture
U937 cells were suspended at a final concentration of 1×106 cells/mL in media and plated on hEC layers pre-treated with 50 μg/mL histone for 1 h. The co-cultured cells were treated with cytosine D–arabinofuranoside (Ara-C; Hospira Pty Ltd., Mulgrave, Australia) for 24 h or without Ara-C for 48 h. Adherent and non-adherent U937 cells were collected separately and stained with FITC-conjugated CD45 (BD Biosciences), PE-conjugated CD105 (BD Biosciences), 7-AAD (Beckman Coulter, Fullerton, CA).
Immunofluorescent Analysis of Tumor Vasculature and Immune Cells
Cell Isolation and Flow Cytometry
Multiparameter Flow Cytometry Profiling
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