Hexokinase method

Manufactured by Beckman Coulter

Sourced in United States

The Hexokinase method is a laboratory procedure used to measure the concentration of glucose in a sample. It relies on the enzymatic conversion of glucose to glucose-6-phosphate by the enzyme hexokinase. The resulting glucose-6-phosphate is then oxidized, and the amount of NADH produced is directly proportional to the glucose concentration in the original sample.

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Lab products found in correlation

Prism software, by GraphPad (1 mentions) Glomax luminometer, by Promega (1 mentions) High sensitivity glp 1 active elisa kit, by Merck Group (1 mentions) Variant 2 turbo system, by Bio-Rad (1 mentions) Chemiluminescence immunoassay, by Beckman Coulter (1 mentions) Eclia, by Roche (1 mentions) Glycerol, by Randox (1 mentions)

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Hexokinase (2 citations)

6 protocols using hexokinase method

Comprehensive Serum and Urinary Biomarker Analysis

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Blood sampling was conducted by vein cannula. Serum insulin was determined by photometry (Beckman Coulter, Brea, CA, USA). Glucose was measured using hexokinase method (Beckman Coulter, Brea, CA, USA). Blood samples to analyze active GLP-1 were withdrawn directly into BD P800 plasma tubes (Becton Dickinson, Heidelberg, Germany) containing an inhibitory mix (i.e., Dipeptidyl peptidase 4 inhibitor), kept in ice and centrifuged in a refrigerated centrifuge within 10 min. Aliquots were stored at −80 °C. Circulating levels of active GLP-1 (7-36) were quantified at the University of Tübingen using the chemiluminescent high sensitivity GLP-1 Active ELISA Kit (#EZGLPHS-35K, Merck Millipore, Darmstadt, Germany). Measurements were performed in duplicate on the Glo-Max Luminometer (Promega GmbH, Mannheim, Germany) applying the five-parameter logistic (GraphPad Prism Software, La Jolla, CA, USA) according to the manufacturer’s instructions. Urinary c-peptide was analyzed with luminescence immunoassay.

Keller J., Kahlhöfer J., Peter A, & Bosy-Westphal A. (2016). Effects of Low versus High Glycemic Index Sugar-Sweetened Beverages on Postprandial Vasodilatation and Inactivity-Induced Impairment of Glucose Metabolism in Healthy Men. Nutrients, 8(12), 802.

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Biochemical Markers of Metabolic Health

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Plasma glucose concentrations (mmol/L) were measured using the hexokinase method (Beckman Coulter). Glycated haemoglobin (%) was measured using high performance liquid chromatography (HPLC) (Variant™ II Turbo System, BioRad, Hercules, CA, USA). Insulin (mIU/L) was measured using a paramagnetic particle-based chemiluminescent system. The lipid profile (mmol/L) was analysed using an enzymatic selective protection-endpoint assay (Beckman Coulter) for LDL-cholesterol, an enzymatic immuno-inhibition-endpoint assay (Beckman Coulter) for HDL-cholesterol, and a glycerol phosphate oxidase in the presence of peroxidase (GPO-POD) endpoint assay (Beckman Coulter) for triglycerides.

Matsha T.E., Raghubeer S., Tshivhase A.M., Davids S.F., Hon G.M., Bjørkhaug L, & Erasmus R.T. (2020). Incidence of HNF1A and GCK MODY Variants in a South African Population. The Application of Clinical Genetics, 13, 209-219.

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Insulin Resistance Evaluation in Fasting Samples

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Fasting blood samples were analyzed by the Colorado Clinical and Translational Sciences Institute core laboratory. Blood glucose was measured via the Hexokinase method (Beckman Coulter) and insulin was measured via chemiluminescence immunoassay (Beckman Coulter).
The Homeostatic Model for Assessment of Insulin Resistance (HOMA-IR) was derived from fasting blood glucose and insulin values [24 (link)] and used in the present analyses as an estimate for insulin resistance. HOMA-IR, glucose and insulin values were centered and scaled using the study sample means and standard deviations for each measure and used in all statistical models.

Shapiro A.L., Wilkening G., Aalborg J., Ringham B.M., Glueck D.H., Tregellas J.R, & Dabelea D. (2019). Childhood Metabolic Biomarkers Are Associated with Performance on Cognitive Tasks in Young Children. The Journal of pediatrics, 211, 92-97.

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Insulin and Glucose Measurement Protocol

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Blood was centrifuged within 5 min after withdrawal and serum as well as plasma supernatants were kept at − 80 °C until assay. Serum insulin concentrations were measured by electrochemical luminescence immunoassay (ECLIA, Roche) with an intra-assay coefficient of variation (CV) < 5.3% and an inter-assay CV < 2.0%. Plasma glucose levels were measured by the hexokinase method (Beckman Coulter) with an intra-assay CV < 0.7% and an inter-assay CV < 1.3%.

Richter J., Thordsen N., Duysen K, & Oltmanns K.M. (2021). Exiguous premeal saccharide intake reduces subsequent food intake in men. European Journal of Nutrition, 60(7), 3887-3895.

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Measurement of Glucose, Insulin, and C-peptide

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Assays for glucose, insulin and C-peptide were performed at the Endocrinology/Lipoprotein Laboratory at the University of Tennessee Health Science Center. Glucose levels were analyzed by the hexokinase method (Beckman-Coulter, Los Angeles, CA). Insulin and C-peptide levels were measured by a two-site sequential chemiluminescent immunometric assays as previously described (20 (link))

Stefanovski D., Vellanki P., Smiley-Byrd D.D., Umpierrez G.E, & Boston R.C. (2020). Population Insulin Sensitivity from Sparsely Sampled Oral Glucose Tolerance Tests. Metabolism: clinical and experimental, 110, 154298.

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Plasma Biochemical Analysis of Metabolites

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Plasma biochemical analysis was carried out on fasting and postprandial samples. Analyses of triglycerides (Randox, Crumlin, Dublin, Ireland), free fatty acid (FFA) (Randox, Crumlin, Dublin, Ireland), glycerol (Randox, Crumlin, Dublin, Ireland), β-hydroxybutyrate (β-HB) (Randox, Crumlin, Dublin, Ireland), cholesterol and HDL cholesterol (Beckman Coulter, Brea, CA, USA) were performed on an Olympus AU400 robot (Centre d’ Explorations Fonctionnelles—Imagerie (CEFI), Bichat, Paris, France). Glucose was analysed by the hexokinase method (Beckman Coulter, Brea, CA, USA) and insulin was measured by enzyme-linked immunosorbent assay (ELISA, Mercodia, Uppsala, Sweden).

Khodorova N.V., Rietman A., Rutledge D.N., Schwarz J., Piedcoq J., Pilard S., Siebelink E., Kok F.J., Tomé D., Mensink M, & Azzout-Marniche D. (2021). Urinary Medium-Chained Acyl-Carnitines Sign High Caloric Intake whereas Short-Chained Acyl-Carnitines Sign High -Protein Diet within a High-Fat, Hypercaloric Diet in a Randomized Crossover Design Dietary Trial. Nutrients, 13(4), 1191.

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