Anti mouse lyve 1 rabbit polyclonal antibody

Histological sections were prepared by standard procedures and immunostained for blood vessels or lymphatics as described previously [40 (link)]. CD31 and LYVE-1 were used as markers of blood and lymph vessel endothelial cells, respectively. An anti-mouse CD31 rabbit polyclonal antibody (Abcam, Cambridge, UK) or an anti-mouse LYVE-1 rabbit polyclonal antibody (Abcam) was used as primary antibody. Quantitative studies were carried out on preparations cut through the central regions of tumors, and three sections of each staining were analyzed for each tumor. Microvessels were defined and scored manually as described elsewhere [36 (link), 41 (link)]. Peripheral vessel density was determined by counting vessels located within a 1-mm-thick rim in the tumor periphery [40 (link)]. BVD and LVD were scored as number of vessels per mm² of viable tumor tissue.

Histological sections were prepared by standard procedures and stained with hematoxylin and eosin (HE) or immunostained for hypoxia, blood vessels, or lymphatics. Pimonidazole [1-[(2-hydroxy-3-piperidinyl)-propyl]-2-nitroimidazole], injected as described earlier [78 (link)], was used as a marker of tumor hypoxia, and CD31 and LYVE-1 were used as markers of blood and lymph vessel endothelial cells, respectively. An anti-pimonidazole rabbit polyclonal antibody (Professor James A. Raleigh, University of North Carolina, Chapel Hill, NC, USA), an anti-mouse CD31 rabbit polyclonal antibody (Abcam, Cambridge, UK), or an anti-mouse LYVE-1 rabbit polyclonal antibody (Abcam) was used as primary antibody. Quantitative studies were carried out on preparations cut through the central regions of tumors, and three sections of each staining were analyzed for each tumor. Blood vessel density was scored by counting CD31-positive vessels in whole tumor cross-sections as described elsewhere [54 (link)]. The density of peritumoral lymphatics was assessed by counting LYVE-1-positive vessels in the muscle tissue located within a distance of 0.5 mm from the tumor surface. Fraction of pimonidazole-positive tissue was assessed by image analysis [44 (link)] and was defined as the area fraction of the viable tissue showing positive staining.