D6546 500ml

Manufactured by Merck Group

D6546-500ML is a laboratory equipment product. It is a container that holds 500 milliliters of a specific substance or solution. The core function of this product is to provide a standardized and measured container for storage and handling of liquids in a laboratory setting.

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Lab products found in correlation

G7513 100ml, by Merck Group (2 mentions) P0781 100ml, by Merck Group (2 mentions) F7524 500ml, by Merck Group (2 mentions) Mcf 7 cell line, by American Type Culture Collection (2 mentions) Leupeptin, by Merck Group (1 mentions) Fbs s 0615, by Bio&Sell (1 mentions) Hep3b, by Merck Group (1 mentions)

3 protocols using d6546 500ml

Hepatitis E Virus Cell Culture Model

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Cell lines used included Huh-7,³⁸ (link) A549/D3,³⁹ and persistently HEV-infected A549 cells⁴⁰ (link) representing parental A549 cells initially infected with HEV genotype 3c isolate 47832c. These cells were chosen as cell culture model as they maintain viral infection while being passaged or cryopreserved, yielding a stable infection, which avoids establishing an infection at each experiment. This feature is superior to other, hepatocyte-based systems and yields high titers and HEV infection efficiency. Cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) (supplemented with 2 mM L-glutamine, 100 μg/mL streptomycin, 100 U/mL penicillin, and 10% v/v fetal bovine serum; FBS.S 0615, Bio & Sell GmbH, Feucht, Germany) at 37°C with 95% relative humidity and 5% CO₂.
Treatments with LDL (360-10-0.1; Lee Biosolutions, Maryland Heights, MO), 25-HC (11097; Cayman Chemicals, Ann Arbor, MI) and simvastatin (sc-200829B; Santa Cruz Biotechnology, Heidelberg, Germany) were performed in serum-free DMEM (D6546-500ML; Sigma Aldrich, Schnelldorf, Germany). Fully supplemented DMEM was used for treatment with gemfibrozil, fenofibrate, avasimibe, PSC833, FGF19, and alirocumab. A final concentration of 200 μM leupeptin (L2884-1mg; Sigma-Aldrich, St Louis, MO) over 24 hours was used to inhibit lysosomal degradation.

Glitscher M., Martín D.H., Woytinek K., Schmidt B., Tabari D., Scholl C., Stingl J.C., Seelow E., Choi M, & Hildt E. (2021). Targeting Cholesterol Metabolism as Efficient Antiviral Strategy Against the Hepatitis E Virus. Cellular and Molecular Gastroenterology and Hepatology, 12(1), 159-180.

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Culture and Compound Treatment of Human Cell Lines

Cited 1 time

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Human cell lines (RCC4, HeLa, Hep3B, MCF-7 and U2OS) were cultured in DMEM (D6546-500ML; Sigma Aldrich) each supplemented with 10% fetal bovine serum (F7524-500ML; Sigma Aldrich), 2 mM L-glutamine (G7513- 100ML; Sigma Aldrich), 50 units/ml of penicillin, and 50 μg/ml of streptomycin (P0781-100ML; Sigma Aldrich). The RCC4 cell line was a gift from C.H.C.M. Buys (University of Groningen, [2 (link)]), the HeLa and Hep3B cell lines were from the European Collection of Cell Cultures (ECACC) [2 (link)], the MCF-7 cell line was from the American Type Culture Collection (ATCC) and the U2OS cell line was a gift from S.Galey (ICRF Clare Hall Laboratories, United Kingdom). Cells were treated either with DMSO (control) or compounds (dissolved in DMSO) and added directly into the cell culture medium to the desired final concentration for 4–5 h as previously described [9 (link),22 (link)].

Chan M.C., Atasoylu O., Hodson E., Tumber A., Leung I.K., Chowdhury R., Gómez-Pérez V., Demetriades M., Rydzik A.M., Holt-Martyn J., Tian Y.M., Bishop T., Claridge T.D., Kawamura A., Pugh C.W., Ratcliffe P.J, & Schofield C.J. (2015). Potent and Selective Triazole-Based Inhibitors of the Hypoxia-Inducible Factor Prolyl-Hydroxylases with Activity in the Murine Brain. PLoS ONE, 10(7), e0132004.

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Cell Culture Conditions for MCF-7, Hep3B, and U2OS

Cited 2 times

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Human cell lines (MCF-7, Hep3B, and U2OS) were cultured in Dulbecco's modified Eagle's medium (DMEM and D6546–500ML; Sigma) each supplemented with 10% fetal bovine serum (F7524–500ML; Sigma), 2 mml-glutamine (G7513–100ML; Sigma), 50 units/ml penicillin, and 50 μg/ml streptomycin (P0781-100ML; Sigma). The MCF-7 cell line was from the American Type Culture Collection (ATCC); the Hep3B cell line was from the European Collection of Cell Cultures (ECACC) (12 (link)). The U2OS cell line was a gift from S. Galey (ICRF Clare Hall Laboratories, United Kingdom). Cells were treated either with DMSO (control) or compounds (dissolved in DMSO) and added directly into the cell culture medium to the desired final concentration as described previously (27 (link), 35 (link)). For hypoxia (0.5% O₂) treatment, cells were seeded at least 24 h prior to being incubated for 16 h in an InvivO2 400 hypoxic workstation (Ruskin Technologies, Bridgend, UK).

Chan M.C., Ilott N.E., Schödel J., Sims D., Tumber A., Lippl K., Mole D.R., Pugh C.W., Ratcliffe P.J., Ponting C.P, & Schofield C.J. (2016). Tuning the Transcriptional Response to Hypoxia by Inhibiting Hypoxia-inducible Factor (HIF) Prolyl and Asparaginyl Hydroxylases. The Journal of Biological Chemistry, 291(39), 20661-20673.

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