Selective macrophage depletion was achieved with a single intraperitoneal injection of clodronate (20 mg/mL) according to the manufacturer’s instructions (FormuMax Scientific, Inc, Sunnyvale, CA). After 24 hours, primary HSCs were isolated using the same protocol used in the isolation of hepatic macrophages, and the cell fraction between the upper layer and the 20% OptiPrep gradient were collected without contamination from the pellets. After centrifugation, the cells were seeded into culture plates in Dulbecco's modified Eagle medium containing fetal serum at 37°C. The culture medium was changed and the RNA was isolated from primary HSCs.59 (link)
Clodronate
Manufactured by Formumax Scientific
Sourced in United States
Clodronate is a synthetic chemical compound commonly used in laboratory settings. It functions as a bisphosphonate, a class of drugs that inhibit bone resorption. Clodronate is utilized in research applications that involve the study of bone metabolism and related physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using clodronate
1
Isolation of Primary Hepatic Stellate Cells
2
Bleomycin-Induced Dermal Fibrosis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We induced dermal fibrosis in mice by bleomycin as previously described [29 (link)]. Bleomycin was dissolved in saline at 1 mg/ml. The saline or bleomycin was administered subcutaneously into the shaved back of the mice (male 8-week-old SCID mice). The administration of saline or bleomycin in the same site was carried out daily for up to 2 weeks. In other experiments, the saline, bleomycin, saline plus clodronate (FormuMax Scientific, CA, USA), or bleomycin plus clodronate were similarly administered to the mice (male 8-week-old C57BL/6 J mice). The administration of saline or bleomycin in the same site was carried out daily for up to 2 weeks, and the administration of clodronate was carried out intraperitoneally every 3 days for up to 2 weeks. In addition, the saline or bleomycin was similarly administered to the mice (male 8-week-old C57BL/6 J mice). The administration of saline or bleomycin in the same site was carried out daily for up to 2 weeks, and the administration of control IgG (2 mg/kg) or anti-IL-4Rα antibodies (2 mg/kg) (BD Biosciences, NJ, USA) was administered intraperitoneally every week for up to 2 weeks.
3
Depletion of Neutrophils and Macrophages in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To deplete neutrophils, mice were injected intraperitoneally (i.p.) with either 200 μg of anti-Gr1 antibody (volume of 100 μl, clone RB6-8C5, BioXCell, USA) or a control injection of the same volume of PBS 24 hours prior to the start of the study. Injections continued every other day throughout the duration of the study. To deplete macrophages, mice were injected intravenously with 100 μl of liposome-encapsulated clodronate (FormuMax Scientific, USA) 24 hours prior to the start of the study, PBS and liposome-encapsulated PBS were used as negative control.
4
Clodronate-mediated Macrophage Depletion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To deplete hepatic macrophages, mice were intraperitoneally administered 140 µg/body clodronate or control liposomes (FormuMax Scientific, Sunnyvale, CA, USA) 24 h prior to surgical interventions.
5
Clodronate Effects on Post-MI Arrhythmia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This study was approved by the medical ethics committees of Sichuan Provincial People’s Hospital (No. 2018–069 and 2019–252) and West China Second University Hospital (No. 2020–009), China. The animal experimental protocols adhered to NIH guidelines. MIF-knockout (KO) mice (Jackson, Bar Harbor, ME, USA) were produced with a background of C57BL/6 mice. We used C57BL/6 wild-type (WT) mice as controls. All mice were raised in an environment with 12/12-h light/dark cycles, and ample food and water were provided. Mice at 8–10 weeks old were used for MI or sham surgery. Mice were anesthetized with 2% isoflurane and then intratracheally intubated. The third intercostal space was incised to expose the heart. Ligation of the left anterior descending coronary artery was performed to induce MI. Sham-operated mice only underwent thoracotomies without coronary arterial ligation.
To further study the effect of infiltrating macrophages on VAs after MI, each mouse received a tail venous injection with clodronate (FormuMax Scientific, USA) or liposome-vehicle at 200 μL on day 1 before surgery, and days 1 and 3 after surgery.
To further study the effect of infiltrating macrophages on VAs after MI, each mouse received a tail venous injection with clodronate (FormuMax Scientific, USA) or liposome-vehicle at 200 μL on day 1 before surgery, and days 1 and 3 after surgery.
6
Clodronate Liposomes in Tenotomy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Clodronate and control liposomes were purchased from FormuMax Scientific (Sunnyvale, CA, #F70101C-AC). One day before the tenotomy, the mice were intraperitoneally injected with 0.15 mL of either Clodronate-or control-liposomes. Subsequently, liposomes (0.1 mL) were administered at specific time points. For the 7-day samples, liposomes were administered at 1, 4, and 6 days posttenotomy. For the 4-day samples, liposomes were administered at 1 and 3 days post-tenotomy.
7
Deciphering Malaria Pathogenesis Through Cell Depletion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were infected with 106Plasmodium chabaudi AS (MRA-429; MR4 Stock Center) parasitized RBCs, and parasitemia was monitored by thin film blood smear as described [86 ]. Where noted, mice were injected i.p. with the following: 300 μg α-CD4 (GK1.5) or isotype control (LTF2); 500 μg α-MCSF (5A1) or isotype control (HRPN) (all BioXCell); or 300 μL liposomes loaded with PBS or clodronate at neutral pH (FormuMax Scientific). To deplete CD169+ cells, heterozygous CD169+/DTR mice were treated 12 d.p.i. with a single i.p. dose (80 ng/g) of DT (Sigma D0564) or a catalytically inactive point mutant (DT*Glu; Sigma D2189). To deplete Lyz2+ cells, including bone marrow macrophages, Lyz2Cre/Cre; Rosa26::STOPfl/fl::DTR mice were treated with 500 ng DT or DT*Glu on d 13 and d 15 post-infection.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!