ihNPCs were seeded at a density of 2.5 × 104 per well in laminin-coated 96-well plates (Corning, NY, USA). After 24 h incubation, the culture medium was changed and MeHg was then added at the concentration ranging from 0 nM, 10 nM and 50 nM, respectively, and cultured for 24 h. The effect of MeHg on cell proliferation was measured using Cell-Light EdU Apollo Kit (RiboBio, Guanzhou, China), as described previously [45 (link)]. The results were expressed as EdU+ cell numbers per 100 4′,6-diamidino-2-phenylindole (DAPI) (RiboBio, Guanzhou, China) stained cells.
Laminin coated 96 well plates
Manufactured by Corning
Sourced in United States
Laminin-coated 96-well plates are a laboratory product designed to provide a specialized surface for cell culture applications. The plates have a uniform coating of the extracellular matrix protein laminin, which is known to support cell attachment and growth. These plates are intended for use in a variety of cell-based assays and experiments where a laminin-rich environment is desired.
Automatically generated - may contain errors
3 protocols using laminin coated 96 well plates
1
MeHg Inhibits Neural Progenitor Proliferation
2
Methylmercury Toxicity in Neural Progenitors
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MeHg (Sigma-Aldrich) was dissolved in dimethyl sulfoxide (DMSO). ihNPCs were seeded at a density of 2.5 × 104 per well in laminin-coated 96-well plates (Corning Inc., New York, NY, USA). After 24 h incubation, the culture medium was changed and MeHg (0 nM, 10 nM and 50 nM) was added and maintained for another 24 h. All experiments were performed in triplicates and repeated at least three times.
3
Neuroprotective Effects of Exendin-4 and DA-CH5 in 6-OHDA-Induced SH-SY5Y Cell Model
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The SH-SY5Y cell line was purchased from the Cell Bank of Chinese Academy of Sciences. Cells were maintained in Dulbecco’s modified Eagle’s medium/F12 (1:1; Boster Biotechnology, Wuhan, China) supplemented with 10% heat-inactivated fetal bovine serum (Cell Max, Beijing, China) and 1% penicillin/streptomycin (Solarbio, Beijing, China) in a 5% CO2humidified atmosphere at 37°C. The 6-OHDA (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 0.02% ascorbic acid and was freshly prepared for each experiment. After 6 hours of serum starvation in serum-free Dulbecco’s modified Eagle’s medium/F12 (1:1), the cells were seeded in laminin-coated 96-well plates (Corning, Shanghai, China) at a density of 5 × 104cells/well for 24 hours. The cells were co-treated with different concentrations of 6-OHDA (0, 150, 250, 350, and 450 µM) for 24 hours in the presence or absence of 100 nM exendin-4 or DA-CH5.
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