A1r a1 confocal laser microscope system

Manufactured by Nikon

Sourced in Japan

The Nikon A1R-A1 confocal laser microscope system is a high-performance imaging solution designed for advanced microscopy applications. It features a fast and sensitive scanning system, enabling rapid image acquisition and high-resolution imaging. The system utilizes a confocal architecture, which allows for optical sectioning and the elimination of out-of-focus information, providing clear and detailed images of samples.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (10 mentions) Cell culture dishes with glass bottoms, by NEST Biotechnology (3 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Live dead kit, by Thermo Fisher Scientific (2 mentions) Goat serum, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg fab2 alexa fluor 488, by Cell Signaling Technology (1 mentions) Cell culture dishes, by NEST Biotechnology (1 mentions) Facscanto 2, by BD (1 mentions) Glass bottoms, by NEST Biotechnology (1 mentions) Nis elements software, by Nikon (1 mentions) A11029, by Thermo Fisher Scientific (1 mentions) A21429, by Thermo Fisher Scientific (1 mentions) Differentiation media, by PromoCell (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Tox7 kit, by Merck Group (1 mentions) Flexstation3 spectrophotometer, by Merck Group (1 mentions) Flexstation3 spectrophotometer, by Molecular Devices (1 mentions) Triton x 100, by Merck Group (1 mentions) Fitc phalloidin, by Merck Group (1 mentions) Paraformaldehyde solution, by Merck Group (1 mentions)

Close spelling variants of this product

A1R confocal microscope (1179 citations) A1 confocal microscope (1115 citations) A1R microscope (119 citations) Laser confocal microscope (111 citations) A1 confocal laser microscope (109 citations) A1 microscope (101 citations) A1R confocal system (69 citations) A1R laser confocal microscope (60 citations) A1R confocal (60 citations) A1 confocal system (58 citations)

24 protocols using a1r a1 confocal laser microscope system

Immunofluorescence Staining and Confocal Microscopy

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After being washed twice with PBS, the cells were fixed in paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100, and blocked with 1% bovine serum albumin (BSA) in PBS for 1 h. The cells were then incubated with the appropriate primary antibodies and secondary antibodies. Cell nuclei were stained with DAPI (Vector Labs). The cells were visualized using an A1R-A1 confocal laser microscope system (Nikon).

Cui H.Y., Wang S.J., Song F., Cheng X., Nan G., Zhao Y., Qian M.R., Chen X., Li J.Y., Liu F.L., Zhu Y.M., Tian R.F., Wang B., Wu B., Zhang Y., Sun X.X., Guo T., Yang X.M., Zhang H., Li L., Xu J., Bian H.J., Jiang J.L, & Chen Z.N. (2021). CD147 receptor is essential for TFF3-mediated signaling regulating colorectal cancer progression. Signal Transduction and Targeted Therapy, 6, 268.

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Immunofluorescence Microscopy of HCC Cells

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Twenty-four hours after siRNA transfection or inhibitor treatment, HCC cells were harvested and allowed to attach for 24 h to Matrigel-pre-coated cell culture dishes with glass bottoms (NEST Biotechnology, Wuxi, China). After washing twice with PBS, the cells were fixed in paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100, and blocked with 1% BSA in PBS for 1 h. The dishes were first incubated with the indicated antibodies for 1 h, washed twice with PBS, and then incubated with Alexa 488-phalloidin solution (1:40) and the corresponding FITC-conjugated secondary antibodies for 30 min in the dark. Cell nuclei were dyed with DAPI (Vector Laboratories, Burlingame, CA). After washing, the dishes were covered with an anti-fade reagent to prevent quenching of the fluorophores, and the cells were visualized using an A1R-A1 confocal laser microscope system (Nikon, Tokyo, Japan).

Wang S.J., Cui H.Y., Liu Y.M., Zhao P., Zhang Y., Fu Z.G., Chen Z.N, & Jiang J.L. (2014). CD147 promotes Src-dependent activation of Rac1 signaling through STAT3/DOCK8 during the motility of hepatocellular carcinoma cells. Oncotarget, 6(1), 243-257.

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Immunofluorescent Staining of PPARγ

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Cells were incubated with canola extracts (as detailed above) in ADM for seven days, after this period cells were fixed with 3% paraformaldehyde (PFA), rinsed with phosphate buffer saline (PBS), then treated with 0.1% Triton X-100 for 7 min at room temperature (RT), and rinsed again with PBS. The cells were then incubated for 30 min in blocking buffer prepared by mixing 5% goat serum (Gibco^®, Eggenstein, Germany) in PBS. The cells were then incubated with anti-PPARγ (81B8) rabbit monoclonal antibody (1:50) Cell Signalling Technology (Danvers, MA, USA) for one hour at ambient temperature, then washed gently with PBS and incubated in the dark with anti-rabbit IgG (Fab 2)–Alexa Fluor^® 488 (1:100; Cell Signalling Technology) for one hour. Finally, they were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) counterstain. Cells were observed using an A1R⁺/A1⁺confocal laser microscope system (Nikon, NY, USA).

Hussain S., Rehman A.U., Luckett D.J., Blanchard C.L., Obied H.K, & Strappe P. (2019). Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis. International Journal of Molecular Sciences, 21(1), 1.

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Immunofluorescence Staining of Cultured Cells

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Cells were harvested and allowed to attach for 24 h to cell culture dishes with glass bottoms (NEST, Wuxi, China). After being washed twice with PBS, the cells were fixed in paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100, and blocked with 1% bovine serum albumin in PBS for 1 h. The cells were then incubated with the indicated primary antibodies for 1 h, washed twice with PBS, and incubated with the appropriate secondary antibodies according to the manufacturer’s instructions. Cell nuclei were stained with DAPI (Vector Labs, CA, USA). After washing, the cells were visualized using an A1R-A1 confocal laser microscope system (Nikon).

Nan G., Zhao S.H., Wang T., Chao D., Tian R.F., Wang W.J., Fu X., Lin P., Guo T., Wang B., Sun X.X., Chen X., Chen Z.N., Wang S.J, & Cui H.Y. (2022). CD147 supports paclitaxel resistance via interacting with RanBP1. Oncogene, 41(7), 983-996.

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Immunofluorescence Imaging of Cellular Proteins

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Immunofluorescence was performed as described previously [8 (link)]. Briefly, cells were harvested and allowed to attach for 24 h to fibronectin-pre-coated cell culture dishes with glass bottoms (801002, NEST Biotechnology Co., LTD.). After washing twice with PBS, the cells were fixed in paraformaldehyde in PBS, permeabilized with 0.1 % Triton X-100, and blocked with 1 % BSA in PBS for 1 h. The dishes were first incubated with the indicated antibodies for 1 h, washed twice with PBS, and then incubated with Alexa 488-phalloidin solution and the corresponding FITC-conjugated secondary antibodies for 30 min in the dark. Cell nuclei were dyed with DAPI (Vector Labs). After washing, the cells were visualized using an A1R-A1 confocal laser microscope system (Nikon, Japan).

Chen R., Wang S.J., Zhang Y., Hou R., Jiang J.L, & Cui H.Y. (2016). CD147 promotes cell motility via upregulation of p190-B RhoGAP in hepatocellular carcinoma. Cancer Cell International, 16, 69.

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Immunofluorescence Staining of Sk-Hep1 Cells

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Upon sacrifice, Sk-Hep1 tumor cells were harvested for immunofluorescence. After washing twice with PBS, the cells were fixed in paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100, and blocked with 1% BSA in PBS for 1 h. The dishes were first incubated with the indicated antibodies for 1 h, washed twice with PBS, and then incubated with Alexa 488-phalloidin solution (1:40) and the corresponding FITC-conjugated secondary antibodies for 30 min in the dark. Cell nuclei were dyed with DAPI (Vector Laboratories, Burlingame, CA). After washing, the slides were treated with an anti-fade reagent to prevent quenching of the fluorophores, and the cells were visualized using an A1R-A1 confocal laser microscope system (Nikon, Tokyo, Japan).

Su Y.H., Huang W.C., Huang T.H., Huang Y.J., Sue Y.K., Huynh T.T., Hsiao M., Liu T.Z., Wu A.T, & Lin C.M. (2016). Folate deficient tumor microenvironment promotes epithelial-to-mesenchymal transition and cancer stem-like phenotypes. Oncotarget, 7(22), 33246-33256.

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Immunofluorescence Staining of F4/80 in Fixed Cells

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Cells were fixed in 4% PFA, permeabilized with 0.2% Triton X-100, and blocked with 10% BSA. The cells were then incubated with F4/80 antibody overnight at 4°C and with Alexa Fluor 555-conjugated secondary antibody (4417, Cell Signaling Technology) for 1 h at 37°C. Cell neutral lipids were stained with Bodipy, and cell nuclei were dyed with DAPI. Images were captured using an A1R-A1 confocal laser microscope system (Nikon, Tokyo, Japan).

Lv J.J., Wang H., Cui H.Y., Liu Z.K., Zhang R.Y., Lu M., Li C., Yong Y.L., Liu M., Zhang H., Zhang T.J., Zhang K., Li G., Nan G., Zhang C., Guo S.P., Wang L., Chen Z.N, & Bian H. (2021). Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis. Frontiers in Cell and Developmental Biology, 8, 609090.

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Immunofluorescence Staining Protocol for Cell Analysis

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Immunofluorescence staining was performed as previously described (20 (link)). Briefly, cells were inoculated into cell culture dishes (NEST, Wuxi, China). After being washed with PBS, the cells were fixed with 2% paraformaldehyde at 25 ℃ for 10 min, permeabilized with 0.1% Triton X-100 at 25 ℃ for 2 min, and blocked with 1% bovine serum albumin at 25 ℃ for 30 min. The cells were then incubated with the indicated primary antibodies at 25 ℃ for 90 min, washed three times with PBS, and incubated with the appropriate secondary antibodies at 25 ℃ for 30 min. Cell nuclei were stained with DAPI (Vector Labs, California, USA). Cells were visualized using an A1R-A1 confocal laser microscope system (Nikon, Tokyo, Japan).

Yang T., Fu X., Tian R.F., Cui H.Y., Li L., Han J.M, & Wang S.J. (2023). TFF3 promotes clonogenic survival of colorectal cancer cells through upregulation of EP4 via activation of STAT3. Translational Cancer Research, 12(6), 1503-1515.

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Characterization of Fluorescent Nanosystems

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Example 2

The fluorescence and hyaluronidase activity of the nanosystem of Example 2 were evaluated (FIG. 6A).

1 mL of nanosystem suspension was centrifuged and resuspended in 100 μL of water. 5 μL of the latter solution was then diluted in 100 μL for the flow cytometry studies. The flow cytometry was conducted with a FACSCanto II (Becton Dickinson & Co., N.J., USA) flow cytometer, using an excitation laser at 633 nm. Unconjugated nanoparticles were used as negative control. A Nikon A1R+/A1+ confocal laser microscope system was used for the confocal microscopy analysis. http://www.nikoninstruments.com/Information-Center/Confocal Unconjugated nanoparticles were used as negative control.

The microscopy and cytofluorometry data demonstrate its high level of fluorescence (FIG. 6B, C). Moreover, the turbidimetric data indicate a high level of hyaluronidase activity of the labelled nanosystems (FIG. 7). It can be concluded from both types of data that the two ingredients covalently coupled to the same nanoparticle retain their respective fluorescence and enzyme activities intact. Similar findings are obtained with the nanosystem carrying rHyal_Sk and fluorophore Cy7.

3 different models were used to simulate subcutaneous absorption of the nanosystems.

US11510995B2. Nanosystems for controlled transport of active molecules for diagnostic, prognostic and therapeutic purposes (2022-11-29). FIDIA FARMACEUTICI S.P.A. [IT], NANOGETIC S.L. [ES]. Inventors: Rosario Maria Sanchez Martin [ES], Salvatore Pernagallo [ES], Juan Diego Unciti Broceta [ES], Luciano Messina [IT], Susanna Vaccaro [IT], Laura Pilotto [IT].

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Immunofluorescence Microscopy Analysis Protocol

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Immunofluorescence was performed as described previously [26 (link)]. Briefly, cells were harvested and allowed to attach for 24 h to cell culture dishes with glass bottoms (NEST Biotechnology Co., LTD.). After washing twice with PBS, the cells were fixed in paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100, and blocked with 1% BSA in PBS for 1 h. The cells were first incubated with the indicated antibodies at 4 °C overnight, washed twice with PBS, and then incubated with the corresponding fluorescein-conjugated secondary antibodies for 1 h in the dark. Cell nuclei were stained with DAPI (Vector Labs). After washing, the cells were visualized using an A1R-A1 confocal laser microscope system (Nikon, Japan).

Wang S.J., Chao D., Wei W., Nan G., Li J.Y., Liu F.L., Li L., Jiang J.L., Cui H.Y, & Chen Z.N. (2020). CD147 promotes collective invasion through cathepsin B in hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 39, 145.

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